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Biomedical subjects

A T Black

Publications and source records attributed to A T Black.

5 recordsLinked to original sources
PubMed1986

ODDC and MULTIGRAPH: programs for spectrophotometer data collection and manipulation using a microcomputer.

A software package is described that collects, graphs, performs peak sensing, and prints data from a spectrophotometer interfaced to an Apple II microcomputer with an ADALAB analog-to-digital converter card. Data collection and storage is performed by an interpreted BASIC program with machine language subroutines. Recalling, graphing, and printout of the data is accomplished by a separate compiled BASIC program. Both of these programs, as well as assisting utility programs, are under control of a menu program. The programs take advantage of all available memory in the 64-K Apple II computer to allow for storage of up to 12 600 data points (7 hours of data collection at a sampling rate of 1 sample every 2 s). As the data collection program provides peak sensing, and the capability of annotating various time points, the programs are ideally suited for use in column chromatography.

Chromatography↗
PubMed1986

Sensitive method for detecting viable cells seeded into bone marrow.

A method for marking viable cells which allows highly sensitive detection of the cells in mixed populations is described. Viable tumor cell lines or normal lymphocytes were stained with the supravital DNA stain Hoechst 33342 (H342) and seeded into human bone marrow. Examination of the marrow mixtures with an inverted fluorescence microscope allows detection of a single H342-stained cell in 1 million marrow cells. H342 staining does not cause significant changes in cell viability or in the sensitivity of the cells to complement mediated lysis. Counterstaining the mixture with trypan blue quenches H342 fluorescence in nonviable cells, limiting detection to only viable cells.

Benzimidazoles↗
PubMed1986

Model system for removing neuroblastoma cells from bone marrow using monoclonal antibodies and magnetic immunobeads.

Variables effecting removal of neuroblastoma cells from bone marrow using monoclonal antibodies and magnetic immunobeads were studied. Human neuroblastoma cell lines were labeled with the supravital DNA stain Hoechst 33342, seeded into normal bone marrow, incubated with monoclonal antibodies recognizing neuroblastoma cell surface antigens (HSAN 1.2, antibody 459, antibody 390, BA-1, and Leu-7), and then mixed with magnetic microspheres coated with goat anti-mouse immunoglobulin. Tumor cells that attached to the magnetic immunobeads were then removed from the marrow with magnets. The efficacy of tumor cell removal depended on the amount of monoclonal antibody bound to tumor cells and the immunobead/tumor cell ratio. In addition, two cycles of purging with both monoclonal antibodies and immunobeads was superior to one cycle. Using a cocktail of the five antibodies, 3 to 4 logs of tumor cells could be depleted from marrow with good recovery of viable hematopoietic cells.

Antibodies, Monoclonal↗