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Biomedical subjects

A T Wood

Publications and source records attributed to A T Wood.

4 recordsLinked to original sources
PubMed2000

Reversed-phase high-performance liquid chromatography of catecholamines and indoleamines using a simple gradient solvent system and native fluorescence detection.

A reversed-phase HPLC method using a C18 column and a two-mobile-phase gradient elution system containing only volatile components has been developed for separation of norepinephrine, octopamine, epinephrine, dopamine, dihydroxyphenylalanine, tyramine, tyrosine, serotonin, 5-hydroxytryptophan, N-acetyl-serotonin and tryptophan. Mobile phase A contains 0.05% aqueous trifluoroacetic acid and methanol (97.5:2.5, v/v) and mobile phase B contains 0.05% aqueous trifluoracetic acid and methanol (40:60, v/v). This method has the advantage that the mobile phase can be removed completely, without salt residues, from the eluted fractions thus simplifying further analytical procedures on isolated fractions. The elution profile of standards is related to structural characteristics allowing prediction of retention times of known compounds and insight into possible structural characteristics of unknown components in a mixture. Detection is via native fluorescence using excitation at 220 nm and emission at 320 nm and under the conditions described has a sensitivity range from 2.5 to 25 pmol, although the sensitivity range can be extended depending on the emission wavelength used.

Amines↗
PubMed1991

Acupuncture for bronchial asthma? A double-blind crossover study.

The therapeutic effectiveness of classic Chinese acupuncture was compared with "placebo" acupuncture in 15 patients with stable bronchial asthma. The patients received treatments with real and placebo acupuncture in a randomly ordered, subject and evaluator-blind crossover fashion twice weekly for five weeks. Both real and placebo treatment periods were preceded by three week periods when no acupuncture was administered. Five patients felt better on real treatment, five patients preferred placebo and five did not feel any improvement on either of the two treatments. Treatment with real acupuncture when compared with no treatment and placebo treatment failed to provide any improvement in daily peak flow rates, asthma symptom scores, number of puffs of beta 2-agonist aerosol use, and pulmonary function results.

Acupuncture Therapy↗
PubMed1991

A comparison of rectal indomethacin with placebo for pain relief following spinal surgery.

Rectal indomethacin was compared with placebo in a randomised, double-blind study of 100 patients undergoing spinal surgery, in which postoperative pain scores, pethidine, diazepam and metoclopramide consumption, bleeding time, blood loss and oral fluid and food tolerance were measured. Side-effects of indomethacin and pethidine were compared in the two groups. In the indomethacin group, pain scores were significantly less for all measurements made during the first three postoperative days, pethidine and diazepam consumption were significantly less on all three days, bleeding time was significantly increased, although still within the clinically normal range, intraoperative and postoperative blood losses were not significantly affected, coagulation was not significantly impaired as assessed clinically, patients tolerated oral feeding significantly earlier, there was no significant increase in the incidence of gastro-intestinal side-effects except for diarrhoea, and there was no significant reduction in the incidence of side-effects associated with the use of pethidine.

Administration, Rectal↗