Thyroid-stimulating antibody (TSab) detected in sera of Graves' patients using human thyroid cell cultures.
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Adult↗
Biomedical subjects
A Tanini
Publications and source records attributed to A Tanini.
Thyrotrophin-responsive adenylate cyclase activity in thyroid toxic adenoma.
The adenylate cyclase system was studied in hyperfunctioning autonomous nodules in comparison with normal thyroid tissue. The basal, TSH- and NaF-stimulated adenylate cyclase activities were tested in purified plasma membrane preparations. Basal enzyme activity in membranes from hyperfunctioning nodules was variable and the response to TSH was either normal, low or absent. The present study demonstrates that an intact adenylate cyclase activity, hyporesponsive to TSH, may exist in the cell membrane of the adenoma.
Adenoma↗
Plasma proinsulin-like components and insulin in chronic liver disease.
In order to explain the increase of total IRI frequently observed at basal status, and after glucose administration, in patients with chronic liver disease, plasma proinsulin-like component and insulin levels have been studied in fourteen patients with liver cirrhosis associated or not with clinical or subclinical diabetes mellitus. A significative increase of plasma insulin was observed at basal status and after a glucose load not only in subjects with clinical or subclinical diabetes but also in those patients without carbohydrate abnormalities. This increase is apparently not correlated to any clinical characteristic and is associated in fasting and after glucose load with increased proinsulin-like component levels especially in patients with clinical or subclinical diabetes.
Aged↗
Interaction of VIPergic and cholinergic receptors in human thyroid cell.
The thyroid tissue is innervated by cholinergic and VIPergic nerves. The present study investigated the possible interactions of cholinergic agents with VIP-induced cAMP accumulation and thyroid hormone release in vitro. Carbamylcholine (Cch), acting through the muscarinic receptor increases cellular cGMP content in cultured human thyroid cells incubated with a phosphodiesterase inhibitor. Cch (10 microM) inhibits cellular cAMP accumulation and thyroxine (T4) release induced by vasoactive intestinal peptide (VIP), with or without a phosphodiesterase inhibitor. Cch (10 microM) inhibits 8-bromo-cAMP-induced T4 release from human thyroid slices. 8-Bromo-cGMP inhibits VIP-induced T4 release from human thyroid slices, only in cells incubated without the phosphodiesterase inhibitor. The results indicate that interactions between VIPergic and cholinergic receptors may be of importance in human thyroid cell.
8-Bromo Cyclic Adenosine Monophosphate↗
Endothelin-1 increases intracellular calcium content in bovine parathyroid endothelial cells.
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Animals↗
Interaction between iodothyronines and thyrotropin receptor in human cultured thyroid cells.
In order to verify the existence of a "short-loop" negative feedback between iodothyronines and adenylate cyclase system of human thyroid, we have studied the effect of preincubation with iodothyronines, iodotyrosines, iodothyronine analogues and iodide on TSH-induced cAMP cellular accumulation in normal human thyroid cells in primary culture. Iodide did not produce an inhibitory effect on TSH-dependent adenylate cyclase system both in normal human thyroid plasma membranes and cultured cells. Iodothyronines at a 30-40 microM concentration did not inhibit the TSH-dependent adenylate cyclase activity of human thyroid plasma membranes; however at a 1 microM concentration they were able to inhibit the TSH-dependent cAMP accumulation by cultured cells. Preincubation with iodotyrosines and iodothyronine analogues failed to inhibit the TSH-responsive cAMP accumulation in human thyroid cultured cells.
Adenylyl Cyclases↗