PubMed Health⌕ Search

Biomedical subjects

A Tchernitchin

Publications and source records attributed to A Tchernitchin.

At least 19 recordsLinked to original sources
PubMed1988

Intraarticular progesterone: effects of a local treatment for rheumatoid arthritis.

The antiinflammatory effect of a single intraarticular injection of progesterone was investigated in 12 patients with rheumatoid arthritis. A local, but no systemic decline of inflammation was observed in 10 of them for at least one month. The average local scores of inflammation at Days 7, 14, 21 and 30 after injection were significantly lower than pretreatment scores (p less than 0.000003 for each comparison). In some patients the effect continued for up to 2 months. No important side effects were observed during the following 2 months. However, a more prolonged followup of treated patients is necessary to rule out the late onset of side effects.

Arthritis, Rheumatoid↗
PubMed1982

Theophylline-estrogen interaction in the rat uterus: role of the ovary.

Uterine edema induced by 0.1 microgram/100 g body wt of estradiol further increases in the presence of theophylline. Theophylline alone or in the presence of 0.001, 0.01, 0.1, and 1 microgram/100 g body wt of estradiol increases uterine RNA and protein content 6 h after its administration, as compared with the same doses of estradiol alone. Both phenomena disappear in the ovariectomized immature rat, suggesting that theophylline potentiates the action of gonadotropins, increasing the synthesis of endogenous ovarian estrogens by the immature ovary. Theophylline decreases the number of eosinophils in the blood and concurrently decreases estrogen-induced uterine eosinophilia at doses of 0.01, 0.1, 1, 10, and 30 micrograms/100 g body wt of estradiol. Estrogen binding by uterine eosinophils in vitro increases in the presence of theophylline. This effect of theophylline could also explain the increase in vivo in estrogen-induced uterine edema.

Animals↗
PubMed1980

Radioautography of dexamethasone in rabbit gingiva and buccal mucosa.

The localization of 3H-dexamethasone in gingiva and in buccal mucosa was investigated radioautographically after systemic administration of the labeled glucocorticoid to rabbits. Tissue samples were processed for dry radioautography of diffusible substances. This study demonstrates localization of 3H-dexamethasone in the nuclei of target cells in the gingival epithelium, stromata, and the walls of blood vessels, and to a lesser degree in the buccal mucosa cells. The findings support the possibility of a direct effect of glucocorticoids via specific hormone cytosol-nuclear receptors in oral tissues.

Animals↗
PubMed1979

Colchicine-estrogen interactions.

Colchicine does not block estrogen-induced recognition of uterine blood vessel surface by eosinophils, but interfers with their migration through endothelial lining and therefore blocks estrogen-induced uterine eosinophilia and edema.

Animals↗
PubMed1979

Interaction between theophylline and oestrogen in the rat uterus.

Theophylline alone or in the presence of 0.001, 0.01, 0.1 or 1 microgram oestradiol-17 beta/100 g body wt increase uterine RNA and protein content 6 h after administration. Uterine oedema induced by physiological doses of oestradiol-17 beta was increased further in the presence of theophylline. Theophylline decreased the number of eosinophils in the blood and concurrently decreased oestrogen-induced uterine oesinophilia at doses of 0.01, 0.1, 1, 10 or 30 micrograms oestradiol-17 beta/100 g body wt. Oestrogen binding by uterine eosinophils in vitro increased in the presence of theophylline. This effect of theophylline could explain the increase of oestrogen-induced uterine oedema in vivo.

Animals↗
PubMed1978

Cortisol-induced migration of eosinophil leukocytes to lymphoid organs.

Cortisol induces a migration of eosinophil leukocytes from the blood to several lymphoid organs, such as the spleen, lymph nodes and thymus, but not to other tissues. This migration explains the cortisol-induced blood eosinopenia, which is much more pronounced in intact than in splenectomized animals.

Adrenalectomy↗
PubMed1978

Dynamics of estrogen binding by uterine cells in vivo.

The dynamics of the in vivo binding and release of tritiated estradiol in different uterine cell types are described. The very early binding of estrogens by the cytosol-nuclear and the eosinophil receptor systems is in accordance with the hypothesis that some estrogenic effects are mediated by these receptor systems.

Animals↗
PubMed1977

Effect of propranolol on various parameters of estrogen stimulation in the rat uterus.

Pretreatment with propranolol does not modify the estrogen-induced uterine eosinophilia, the water imbibition effect, nor the increase in uterine RNA and protein content. This confirms the independence of these parameters from the estrogen-induced early increase in uterine cAMP, since, when observed, the latter is suppressed by propranolol pretreatment.

Animals↗
PubMed1976

New concepts on the action of oestrogens in the uterus and the role of the eosinophil receptor system.

Two receptor systems for oestrogens have been demonstrated in the uterus: the cytosol-nuclear receptor system and the eosinophil receptor system. It has been proposed that the cytosol-nuclear receptor system mediates the genomic response to oestrogens in the uterus, while the eosinophil receptor system is thought to mediate the uterine edema and other early oestrogenic responses in the uterus. Cortisol is known to decrease drastically the number of eosinophils in the blood and therefore to limit their availability for migration to the uterus. The present results show that cortisol also drastically reduces both the oestrogen-induced uterine eosinophilia and the uterine wet weight responses, but does not interfere with the oestrogen-induced uterine RNA and protein increases. Oestradiol-17 beta has a higher affinity than oestriol for the cytosol-nuclear receptors and is now found to be the more potent oestrogen in inducing the genomic activation in the uterus. Estriol has a higher affinity than oestradiol-17 beta for the eosinophil receptors, and therefore, oestriol is the stronger oestrogen in inducing those oestrogenic effects which are mediated by the eosinophil receptor system. We conclude that the eosinophil receptor system for oestrogens is a new system, independent of Jensen's cytosol-nuclear receptor system, and this eosinophil receptor system is involved in the mechanism of oestrogen action in the uterus.

Animals↗
PubMed1976

Dynamics of eosinophils in the uterus after oestrogen administration.

To investigase the role of the eosinophil leukocytes in the early oestrogenic responses in the uterus, the kinetics of oestrogen-induced uterine eosinophilia and other parameters of oestrogen stimulation were studied at very early times. Uterine eosinophils increase as early as 5 min after an intravenous injection of oestradiol to immature rats, much earlier than several other changes in the early parameters of oestrogen stimulation. Large number of uterine eosinophils are found attached to the wall of small uterine blood vessels at early times. To elucidate the mechanisms involved in the specific attraction of eosinophils to the uterus in the presence of oestrogens, the in vivo localisation of oestrogens in the rat uterus at early times was studied using a radioautographic technique. Oestrogen receptors were found in the surface of eosinophils and in the wall of small uterine blood vessels. This simultaneous presence of both oestrogen receptors is proposed to explain the specific attachment of eosinophils to uterine blood vessels in the presence of oestrogens, which is the initial step toward eosinophil penetration into the uterus.

Animals↗
PubMed1975

[The binding of estradiol to human polynuclear eosinophilic leukocytes].

High affinity estradiol binding is found in the fraction of human eosinophil rich, polynuclear leukocytes sedimenting between 800 times 10 g times mn and 24,000 times 20 g times mn. The apparent dissociation constant at equilibrium is approximately 0.6 nM, and the total number of binding sites per blood eosinophil leukocyte is 7,400 sites per cell. Hydrogen peroxyde (1 muM) enhances estradiol binding, a finding similar to that previously observed autoradiographically for the binding of estradiol to the eosinophils of rat uterus.

Binding Sites↗