Biomedical subjects

A Tessitore

Publications and source records attributed to A Tessitore.

At least 37 records · Page 2Linked to original sources

PubMed1996

Involvement of an arachidonic-acid-dependent pathway in the interferon-beta-mediated expression of C202 gene in Ehrlich-ascites-tumor cells.

We have investigated the signal transduction mechanism of the expression of the C202 gene mediated by interferon beta (IFN-beta) in the murine Ehrlich's ascites tumor cell line. We have shown that treatment of cells with IFN-beta transiently enhances within minutes the release of free arachidonic acid through membrane phospholipase activity. Furthermore, prior treatment with either p-bromophenacyl bromide, an antagonist of both cytosolic and secretory phospholipase A2, or neomycin, which blocks phospholipase C activity, significantly decreased the activation of the murine IFN-beta-inducible gene, C202. Moreover, an increase of the expression of the C202 gene was observed after blocking of both the cyclooxygenase and lipoxygenase pathways. This suggests that further metabolism of arachidonic acid to epoxides via epoxygenase-catalysed pathways may be a mechanism by which second messengers for IFN-beta-mediated effects on C202 gene expression are generated. Taken together, these results indicate that lipids as second messengers may be important mediators in the IFN-beta-based activation of C202 gene expression.

Animals↗

PubMed1996

Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation.

Thirty-one tumor samples from selected cases of gastric carcinoma were analyzed for mutations of the p53 tumor suppressor gene. Template DNA was prepared according to the touch preparation procedure, which allowed us to isolate clusters of neoplastic cells out of a stromal cellular background to be used as a template in the amplification of target exons of the p53 locus. In our present study, by polymerase chain reaction/single strand conformation polymorphism analysis we give evidence of p53 mutations occurring in the DNA-binding core domain of the protein (exons 5 through 9), which are clustered in stages III and IV of the disease (six mutations out of seventeen samples; 35%). No p53 mutations were detected in fourteen gastric cancer samples at I and II stages. Beside the use of conventional molecular scanning procedures, our study proposes the application of the touch preparation method to increase the detection of genetic alterations in human solid tumors.

Base Sequence↗

PubMed1993

[Heparin and ischemic stroke].

In absence of careful controlled trials, heparin therapy of ischemic stroke is based today on the clinical experience and personal belief of the physicians. Moreover, the incomplete knowledge of physiopathogenesis of ischemic stroke adds further confusion on those nosographic conditions treatable with heparin. This work aims to report the opinions prevalent in the literature, together with personal convictions, so to supply the reader with a complete view of the scientific discussion on this argument.

Acute Disease↗

PubMed1992

Force and power of preferred and non-preferred leg in young soccer players.

The purpose of this study was to assess the effects of soccer training on strength and power of leg extensor muscles in preadolescent boys and their possible influence in developing a muscular lateral dominance. Twenty male children (mean age 9.6 yr), ten soccer players and ten untrained subjects, participated in this study. Force and power were measured at five constant cranking velocities on an isokinetic bicycle dinamometer. Each isokinetic load was given after each trial. Force and power outputs were calculated for each limb. Soccer players always showed higher and statistically significant differences (p < 0.05) for force and power values for all considered pedal frequencies. No significant differences were observed for force and power values between preferred and non preferred limb. The results demonstrate the effect of soccer training on the increase of force and power of both legs regardless of lateral dominance.

Biomechanical Phenomena↗

PubMed1986