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Biomedical subjects

A Toshkov

Publications and source records attributed to A Toshkov.

At least 55 records · Page 3Linked to original sources
PubMed1978

Effect of experimentally induced trichinellosis on the infection with Erysipelothrix rhusiopathiae in rats.

Infection with Erysipelothrix rhusiopathiae in rats infested 20 days earlier with Trichinella spiralis developed more slowly, the clinical and pathoanatomic changes in the joints were expressed to a less extend, and the mortality rate was lower. The erythrocyte sedimentation rate, the precipitin formation and the phagocytic activity of the macrophages did not considerably change. Experiments carried out to elucidate this fact did not reveal any antigenic or antagonistic relationships between parasite and bacterium nor any protective effect of the host's serum. The inhibitory influence of corticosteroids on the defence forces was not completely manifested in the rats infested. This fact might be explained by interrelations depending both on the cycle of helminth development and on the non-specific immunological reactivity of the organism, the latter being stimulated by the helminth invasion.

Adrenocorticotropic Hormone↗
PubMed1978

Experimental pyelonephritis induced by L-forms of Proteus mirabilis in rats.

Pyelonephritis was produced in 75 white rats by applying a 24-hour ligature on the ureter and by intravenous injection of a suspension of 5X10(9) cells of a stable Proteus mirabilis L-form. Pyelonephritis was proved microscopically in 44% of the animals. The revertants of the introduced L-form play an etiological role in the development of the infection. The stability of the pathohistological findings makes this model suitable for the purpose of experimental chemotherapy.

Animals↗
PubMed1978

Ultrastructural characterization of stable L-form cells from Erysipelothrix rhusiopathiae and of accompanying artifacts.

The stable L-form of Erysipelothrix rhusiopathiae is a typical protoplast type L-form. Cells are surrounded by a trilamellar cytoplasmic membrane only. They grow in form of aggregations in liquid media and their diameters vary between 0.1 and 2 micrometer. Always a large portion of cells undergoes lysis. It seems to be characteristic for L-form cultures of E. rhusiopathiae that always many artifact structures are formed. The artifacts are spherical particles with diameters of 0.1 micrometer to more than 3 micrometer. They can be differentiated from L-form cells only by electron microscopy. The artifacts consist of electron dense amorphous material and their surface is irregular without a clear boundary line. Obviously, these artifacts are produced from protein components of the medium and from cytoplasmatic components of the lysing L-form cells.

Animals↗
PubMed1978

Histoautoradiographic studies on the fate of 3H-thymidine labelled L-forms of E. rhusiopathiae in albino mice.

L-forms of Erysipelotrix rhusiopathiae labelled with 3H-thymidine were intravenously administered to albino mice. Autoradiographic studies of the liver, kidney and spleen were undertaken at periods ranging from 2 minutes to 15 days. On the second minute following the administration of the radioactive material whole labelled microorganisms and chains of silver grains were recovered in the examined organs. Up to the 15th minute labels were observed also in the cells of the RES. Following the 30th minute the silver grains were positioned at a characteristic site in the Golgi region of the hepatocytes. At the same time in the kidney they were localized in the glomerular space and in the lumen of the renal tubules, whereas in the spleen - mainly around the megakaryocytes. By the 15th day labelling gradually diminished, single silver grains being found over some nuclei of megakaryocytes, liver and kidney parenchymal cells. The present study throws light over some aspects of the interrelationship between the micro- and macroorganism concerning the mechanisms of desintegration, elimination and the uptaking of labelled microbial DNA.

Animals↗