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A Tsuboi

Publications and source records attributed to A Tsuboi.

95 records · Page 6Linked to original sources
PubMedDate not supplied

Effects of hyperthermia on mouse L cells irradiated with fractionated X-rays.

The responses to X-rays and radiosensitizing effects of post-treatments with heat were examined in the cells exposed to different doses of multifractionations. When cells were irradiated with daily doses of 2 Gy the radiation responses changed during multifractionated treatments, giving rise to two components in survival curves. The changes may be due to selective killing of radiosensitive cells or a cell progression through radioresistant phases during multifractionation irradiation as shown in analysis of the cell cycle. However, the survival curves became almost exponential when fractionation was performed with daily doses of 3-4 Gy. Radiosensitization by heat at 43 degrees C for 15 min was detectable in the cells after exposures to fractionated doses of more than 7 Gy with daily doses of 2 Gy, but not after exposures to daily doses of 3-4 Gy. Radiosensitization by heat at 43 degrees C for 30 min was manifest as disappearance of shoulder and increase of the slope in survival curves after irradiation with daily doses of 2 Gy. It was observed as an increase of slope in survival curves when cells were irradiated with daily doses of 3 or 4 Gy. Such differences in the radiosensitization may be due to a difference in the susceptibility to heat among the cells which had been exposed to the different doses and regimens of fractionated irradiation.

Animals↗
PubMedDate not supplied

Flow cytometric and Ki-67 immunohistochemical analysis of cell cycle distribution of cervical cancer during radiation therapy.

BACKGROUND: Little is known about the cell kinetics of human solid tumors during radiation therapy. MATERIALS & METHODS: Thirty one patients with cervical squamous cell carcinoma were analyzed by flow cytometry and immunohistochemical staining with Ki-67 antibody for tumor cell kinetics in the early period of radiation therapy. RESULTS: The G0/G1 fraction was significantly larger in stage 3 disease than in stage 2 disease. During radiation therapy, a decrease in the G0/G1 fraction and an increase in the S fraction were observed in stage 3 but not in stage 2 disease. Two reversal patterns of change in the cell cycle appeared in the early period of radiation therapy. The S phase fraction decreased significantly as the G0/G1 fraction of pre-treatment tumors became smaller than 70%, whereas it increased as the G0/G1 fraction became larger than 70%. The S fraction decreased significantly as Ki-67 growth fractions of pre-treatment tumors became greater than 30%, but on the contrary, it increased as the Ki-67 growth fraction became smaller than 30%. Only 2 of 23 patients showed a significant increase of the G2M fraction, reflecting the presence of the G2 block. The Ki-67 growth fraction increased about 26 % at 9 Gy compared with the growth fraction before treatment. Tumors which responded well showed higher G0/G1 fractions and lower S fractions than those which responded badly. CONCLUSION: The change of cell cycle parameters of the tumors during radiation therapy suggests that two reversal patterns of changes in cell kinetics take place in the early period of radiation therapy, which may have a potential impact in the modification of radiation therapy.

Adult↗