Biomedical subjects
Publications and source records attributed to A Tsukada.
After a head-on automobile collision, a 21-year-old man was admitted with respiratory distress and subcutaneous emphysema. Bronchoscopy revealed a disruption of the trachea and a complete transection of the intermediate bronchus. A right posterolateral thoracotomy was performed, and both lesions were reconstructed primarily using absorbable materials.
A rare case of a radiolucent osteoma of the skull is reported. The radiological and pathological findings of this lesion, including magnetic resonance imaging findings, are presented, and the efficacy of magnetic resonance imaging for the diagnosis of this skull tumor is evaluated. The differential diagnosis of radiolucent bone tumors is discussed.
A simple, inexpensive method of portable digital subtraction angiography (DSA) using an image processor (Sigma X), still-videorecorder and control panel combined with a surgical x-ray television unit can provide real time subtraction images on the monitor. This portable DSA unit was used in 161 cases (130 in the operating room and 31 in the intensive care unit). In the operating room it is useful: 1) to confirm patency of the parent artery and its branches after aneurysm clipping, 2) to identify feeding arteries of arteriovenous malformation and to confirm total extirpation, 3) to confirm the patency of extracranial-intracranial bypass, 4) to confirm patency of the internal carotid artery and absence of flap formation after carotid endarterectomy. In the intensive care unit, it is particularly useful for visualizing cerebral vasospasm after subarachnoid hemorrhage and recanalization of an occluded major intracranial artery. Absence of intracranial circulation can be demonstrated in patients with suspected brain death.
In order to perform carotid endarterectomy more safely, we have evaluated, intraoperatively, the result of an operation with electromagnetic flowmeter and portable digital subtraction angiography (DSA). During surgery, flow of the exposed internal and common carotid artery was measured with an electromagnetic flowmeter, before and after endarterectomy. After the closure of the arteriotomy, portable DSA was carried out by injecting contrast medium from the common carotid artery. Our simple portable DSA system consists of a surgical X-ray television set, image processor (Sigma X) and still video-recorder. This system almost gives us a real-time presentation of a subtracted view of angiography. There were twenty patients including one patient with major stroke, 14 with minor stroke, three with transient ischemic attack, and two asymptomatic patients. Increase of the flow through the internal carotid artery was documented with an electromagnetic flow-meter in 13 of 20 patients following endarterectomy. Flow reduction was seen in two patients, one of whom was found to have an intimal flap from residual plaque visualized in the portable DSA. The plaque was removed by reopening the artery. Among twenty patients, intraoperative portable DSA showed an intimal flap in three patients and a step in four patients. Although the incidence of abnormal findings was high, degree of abnormality was not severe enough to reopen the artery except in one case. The purposes of carotid endarterectomy is two-fold. It is used for the removal of the plaques which can be a source of emboli, and also for the augmentation of flow in the narrowed segment of the artery.(ABSTRACT TRUNCATED AT 250 WORDS)
Histological examination was performed on the oral mucosa of 25 patients with PSS which consisted of 16 cases of Acrosclerotic type (A type) and 9 cases of Diffuse type (D type). Results obtained were as follows: (A) Sclerotic change of connective tissue were found in the submucosal area (a type) and in the salivary peri-glandular++ area (b type) and also in the both areas (c type). (1) In A type of PSS, sclerotic changes was found in 14 out of 16 cases (87.5%) and (2) In D type, 9 out of 9 cases (100%). (3) In D type, sclerotic changes were marked and, moreover, found in the earlier stage of disease more than in A type. (B) Inflammatory change of salivary gland based on the criteria of Chisholm and Mason. (1) Moderate and marked salivary gland adenitis were found in 6 out of 16 cases of A type (37.5%) and 2 out of 9 cases of D type (22%). (2) Adenitis were found more frequently in the early stage of disease in both types. From above described findings, it is concluded that 1) as to sclerotic change, the early and marked changes were found in D type and 2) as to inflammation of salivary gland, no significant difference was found between both types.
A rapid electrophoretic method detecting serum lipoprotein(a) (Lp(a)) was described. When serum lipoproteins were electrophoresed using an agarose gel film containing 0.6% agarose and 3% sucrose, a distinct extra-band was frequently detected between beta- and prebeta-bands. This lipoprotein band was identified as Lp(a) by immunoblotting technique using anti-Lp(a) serum. Using this electrophoretic method, we studied 1,353 subjects with a variety of diseases. The Lp(a)-positive subjects accounted for 8.4% of all. Neither sex nor age influenced the frequency of Lp(a) positivity. Frequencies of definite myocardial infarction in Lp(a)-positive subjects did not differ significantly from those in Lp(a)-negative ones. Those of cerebral infarction in Lp(a)-positive subjects were significantly higher than those in Lp(a)-negative ones. An electrophoretic method described herein is very useful for the rapid detection and screening of Lp(a), and hence for large-scale clinical studies of atherosclerotic risk assessment.
We report two cases of traumatic cerebral vascular disease which were treated successfully with barbiturate. The first case sustained blunt trauma to the bilateral vertebral arteries, resulting in complete occlusion of both arteries. After ligation of the injured vertebral arteries, multiple cerebral infarction appeared. Cerebral angiography revealed dissection and stenosis of the bilateral internal carotid arteries. We treated this case with barbiturate (Thiamylal) in combination with administration of heparin. The second case sustained cerebral contusion and traumatic subarachnoidal hemorrhage as a result of a motor cycle accident. This patient deteriorated and cerebral angiography showed diffuse cerebral arterial vasospasms. When this was treated with induced hypertension, he developed recurrent subarachnoid hemorrhage. In order to protect the brain from ischemia without elevating blood pressure, we employed barbiturate therapy and the patient recovered without major neurological deficit. The condition of severe head injury with cerebral ischemia is complicated. Therefore it has been hard for neurosurgeons to cure the patient with this condition. But we treated it with barbiturate successfully. Barbiturate therapy in severe head injury with cerebral ischemia may decrease the mortality in that group of patients considered difficult to treat with the usual therapeutic modalities.
We studied functioning cells of GH-producing pituitary adenoma quantitatively by the immunohistochemical method using anti GH antibody and compared the result with clinical hormonal data by discussing a clinical significance of the immunohistochemical study of pituitary adenoma. Fifteen acromegalics with pituitary adenoma, who underwent surgical operation followed by immunohistochemical study, were subjected. Volume of the adenoma was calculated on the basis of thin coronal cut CT scanning. The specimen was stained after PAP method. The quantitative analysis was done by investigating the ratio of anti GH antibody positive area in 30 fields (SAR). Using the value obtained, stained volume (SV) was calculated by multiplying total volume by SAR. The SV was regarded as total volume of GH positive cells. These values were compared to the preoperative serum GH levels. The results were as follows: (1) Immunohistochemically, the adenomas were classified into two types, the sporadic type and the diffuse type, by incidence of GH positive cells. SAR ranged from 0.2% to 52.4%, and means of the types were 3.0% and 24.8%, respectively. (2) All the eosinophilic adenomas demonstrated to be the diffuse type and the mean SAR was 23.7%. On the contrary, 75% of chromophobe adenomas were the sporadic type and the mean SAR was 4.9%. (3) There was no significant correlation between the serum GH level and the volume of the adenoma. There was an especially high correlation between the serum GH level and SV with a correlation constant of 0.971. (4) In fibrous adenomas the serum GH level was extremely low for their total volume.
The gene for cellulase from Ruminococcus albus F-40 was cloned in Escherichia coli HB101 with pBR322. A 3.4-kilobase-pair HindIII fragment encoding cellulase hybridized with the chromosomal DNA of R. albus. The Ouchterlony double-fusion test gave a single precipitation line between the cloned enzyme and the cellulase from R. albus. The size of the cloned fragment was reduced by using HindIII and EcoRI. The resulting active fragment had a size of 1.9 kilobase pairs; and the restriction sites EcoRI, BamHI, PvuII, EcoRI, PvuII, and HindIII, in that order, were ligated into pUC19 at the EcoRI and HindIII sites (pURA1). Cellulase production by E. coli JM103(pURA1) in Luria-Bertani broth was remarkably enhanced, up to approximately 80 times, by controlling the pH at 6.5 and by reducing the concentration of NaCl in the broth to 80 mM.
Explore the source record for details and available documents.
The BSD-1000 Hyperthermia System delivers therapeutic heat (hyperthermia) to surface or subsurface and deep seated malignant tumors by the external or interstitial application of electromagnetic energy, and monitors the temperature of target and surrounding tissues by means of invasive temperature probes. In response to an operator-designated control probe, the BSD-1000 Hyperthermia System automatically adjusts power to maintain the operator-set therapeutic temperature. Characterization was described according to the following items mainly based on home data, heating ability temperature measurement environmental effects by the electromagnetic field 4) practical problems. It is important to submit materials so that users could judge which equipments would suit their own purposes. In order to standardize how to express the efficiency in each equipment, collaboration is stressed in every concerned field.
Explore the source record for details and available documents.
We report case of malignant meningioma involving the lumbar vertebra (3rd and 4th). A 53-year-old man who developed occipitalgia in April 1977 was operated under the diagnosis of posterior fossa tumor in August 1977. The tumor was totally removed macroscopically Simpson grade 2. The histological diagnosis was malignant meningioma, He complained of lumbago and right leg pain since June 1981. Destruction and tumor stain of the 3rd and 4th lumbar vertebrae were recognized on CT scans. In May 1982, surgical decompression and spinal fixation were attempted. The surgical specimen was verified as a malignant meningioma. Therefore, he underwent irradiation therapy to the lumbar spine (total dose 50 Gray). He presented with occipitalgia in March 1983. CT scan detected tumor recurrence in the suboccipital region. The recurrent tumor was totally removed immediately. The histological feature was malignant meningioma. The mode and site of metastasizing meningioma are discussed with a review of the literature.
We reported four cases with well demarkated low density area in the middle cranial fossa, which was not enhanced with contrast medium and had the same absorption coefficient as the CSF. The operations and histological examinations revealed that two cases were arachnoid cysts and the others were porencephalic cysts. The clinicoradiological differential clues are listed below. 1) The porencephaly has intimate relation with focal neurological signs. 2) The thinning and bulging of the temporal bone are not a specific finding of an arachnoid cyst. Hemicranial atrophy, elevation of minor sphenoid wing and dilatation of paranasal sinuses in addition to focal bulging and thinning are indicative of porencephalic cyst. 3) Hemiatrophy of the brain and dilatation of the lateral ventricle on CT as well as the absorption coefficient of the brain surface facing the cyst are important finding of the porencephaly in differentiation. 4) The investigation of CSF dynamic is by no means helpful in differentiation between arachnoid cyst and porencephaly except intraarachnoid cyst. 5) The horizontal portion of the middle cerebral artery is not elevated in both two porencephalic cases. One of them showed a slight shift of anterior cerebral artery toward the affected side. An obstruction of the vessel is an important finding in porencephaly. 6) The electroencephalography can clearly demonstrate a porencephalic cyst as a localized slow focus, whereas it is not so remarkable in an arachnoid cyst.
Explore the source record for details and available documents.
43-year-old man developed transverse myelitis following an infection with Mycoplasma pneumoniae. Among the uncommon neurological complications following infection with this organism, transverse myelitis has been reported rarely but may be more frequent than previously thought. Posterior spinal column involvement might result in a permanent neurological deficit despite the usually good prognosis.