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A Uchida

Publications and source records attributed to A Uchida.

12 recordsLinked to original sources

Distribution of mitochondrially inherited drug-resistance genes to tetrads from young zygotes in yeast.

Pairs of strains of opposite mating type were isolated from a strain of Saccharomyces cerevisiae. From these isogenic strains, mitochondrially inherited resistant mutants to antimycin A and erythromycin were isolated. By using the two resistance genes as mitochondrial markers, it was proposed that the distribution of the mitochondrial genomes from zygotes to tetrads seemed not to be random but the genomes from either a or alpha parent would be selected with approximately equal frequencies after zygote formation and subsequently distributed uniparentally to meiotic products.

Antimycin A

Pattern of somatic segregation of the cytoplasmic drug-resistance factors in yeast.

The pattern of somatic segregation of the cytoplasmic factors confering resistances to chloramphenicol, erythromycin and oligomyin in S. cerevisiae was studied. The fractions of the zygotes heterozygous for the chloramphenicol-resistance factor and for the erythromycin-resistance factor decreased exponentially with generation number of zygotes. The rate of the segregation was highest for the chloramphenicol-resistance factor and lowest for the oligomycin-resistance factor. The segregation rate as well as the transmission polarity of the chloramphenicol-resistance factor varied with different carbon sources with which the parental haploids were grown prior to mating.

Chloramphenicol

Appearance of spore coat protein in the cell extracts of Bacillus subtilis asporogenic mutants.

By use of the antigen-antibody techniques we have studied whether asporogenic mutants of Bacillus subtilis can synthesize the spore coat protein. Antibody specific to spore coat protein was prepared and used to demonstrate that the spore coat protein was synthesized at the early stage of sporulation. We report here that asporogenic mutants synthesize the spore coat protein.

Bacillus subtilis