Biomedical subjects

A Uchiumi

Publications and source records attributed to A Uchiumi.

5 recordsLinked to original sources

PubMed1999

Arsenic accumulation in organs of the fresh water fish Tribolodon hakonensis.

The fish Tribolodon hakonensis lives in good health in Lake Usoriko, which has been acidified and naturally contaminated with arsenic by volcanic activities. We have determined the contents of various metals in various fish organs of T. hakonensis, collected at Lake Usoriko. We found that a large amount of arsenic was accumulated in the eye, especially in the choroid-retina part. The arsenic concentrations in the liver and kidneys were about 0.4 microgram/g (wet), whereas the muscle contained less arsenic. The arsenic accumulation in the skin was also observed, especially in the epaxial part. Zinc and arsenic contents in various fish organs correlated well.

Animals↗

PubMed1998

Sensitive detection of trace aluminium in biological tissues by confocal laser scanning microscopy after staining with lumogallion.

This paper describes a method for the sensitive detection of aluminium in biological tissues by real time confocal laser scanning microscopy after staining with lumogallion. The method enabled detection of aluminium > or = 9 micrograms g-1 in bone and is more sensitive than the conventional histochemical methods with aluminium and solochrome azurine, etc. Lumogallion reacts specifically with aluminium to form a fluorescent complex so that the proposed method is useful for detection and identification of aluminium in tissues.

Aluminum↗

PubMed1998

Staining for histologic diagnosis of aluminum osteopathy--application of confocal laser scanning microscopy to observation for offminum in bones from aluminum-treated, subtotally nephrectomized rats.

To histologically detect excess aluminum (Al) taken up by bones in a rat model of renal failure prepared by 5/6-nephrectomy, non-decalcified bone tissue sections from specimens of the tibia, femur, rib and ilium were stained with a newly developed lumogallion reagent and examined for aluminum by confocal laser scanning microscopy. The assay revealed that Al contents in the tibia and femur of Al-treated rats tended to be a few-fold higher, compared to controls. With the lumogallion stain technique, Al in bones could be detected and identified at Al concentrations of > or = 9.0 micrograms/g in bone tissues. The reaction of the metal with lumogallion is specific and proved to be positive even at concentrations of excess Al so low as to be undetectable by conventional methods. The present observation disclosed that, unlike the generally accepted view, the Al was accumulated not in the calcification front but was distributed diffusely or as aggregates at high concentration in osteoid tissues. Z mode scans of the slides permitted steric delineation of the state of Al accumulation in bone tissues. The method described herein is considered a useful means for clinical diagnosis of aluminum osteopathy.

Aluminum↗

PubMed1990

Tooth and bone tissue analysis by element mapping spectrometry.

Elemental analyses of the tooth and bone tissues were carried out by X-ray fluorescence element mapping spectrometry, a technique we have recently developed. This analytical system has the advantage of providing data on distribution of element concentration in a given specimen in overhead view without involving destruction of the tissue architecture. This paper briefly introduces the apparatus of this system and presents analytical data obtained for several elements (calcium, iron, arsenic, zinc and strontium) in the tooth and bone tissues of rats bearing tumor induced with lead acetate. Lead distributed in the maxillary incisive bone and femur was demonstrated in rats with lead acetate-induced tumor sacrificed at 42 weeks after injections. Arsenic observed in the femur of control rats was found replaced by lead in the tumor bearing rats. The findings are of interest as viewed with reference to chelating activity of lead.

Animals↗

PubMed1990

Inorganic elements in the tooth and bone tissues of rats bearing nickel acetate- and lead acetate-induced tumors.

Biological effects of such metals as nickel and chromium alloys used as dental materials, particularly their metabolism and potential toxicity have not been well elucidated. Certain metallic materials are known to be toxic and arsenic, nickel, chromium and lead have been shown to have carcinogenic potential in laboratory animals. This study was performed to assess carcinogenicity and accumulation in tissues of metals in rats. Three-week old male F344/NSle rats were injected s. c. with 30 mg/kg of nickel acetate or 60 mg/kg of lead acetate weekly for 5 weeks after 2 weeks of acclimatization, and were followed by observation for 80 weeks. The group treated with lead acetate showed a greater incidence of tumor, compared with the nickel acetate-treated group. Teeth, femora, other bones and neoplastic tissue from tumor-bearing animals were subjected to elemental analysis by spectrophotometry and ICP. High concentrations of Pb were detected in the mandibula, femur and tumor tissue of rats with Pb-induced tumor. In rats with Ni-induced tumor, Ni was not demonstrable in the mandibula, maxilla or tumor tissue and detected in small quantities in the nasal bone and femur. Thus, Pb proved to be retained in tissue at the site of injection and also markedly accumulated in the tooth and femur as assessed 46 weeks after injections; whereas Ni was no longer detectable in tissue at the injection site, nor in tumor tissue but only small quantities in the said bones.

Acetates↗