[A new method for the early diagnosis of lactation mastitis].
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Electrophoresis, Polyacrylamide Gel↗
Biomedical subjects
A V Andreev
Publications and source records attributed to A V Andreev.
[Ways of reducing mortality in perforated ulcer of the stomach and duodenum].
Based on an analysis of lethal outcomes in 20 of 326 patients with perforated ulcers of the stomach and duodenum the authors recommend to perform planned operations more often in elderly and senile patients, to combine suturing the ulcer with truncal vagotomy, intensive therapy, administration of drugs, catheterization of the superior mesenterial artery.
Adult↗
[Hematologic indicators in patients with morbid obesity after surgical creation of the miniature stomach].
Surgical establishment of miniature stomach was conducted in 28 patients with severe alimentary-constitutional obesity (mean body weight 155.7 kg). After a 18-36-month follow-up the patients underwent hematological examination. It was found that a 134% excess of body weight registered preoperatively dropped to 54%. Reduced levels of blood red cells, hemoglobin, serum iron were not registered. Neither was anemia. This shows that physiological function of the whole gastrointestinal tract was not damaged, the blood loss appeared insignificant. Substitution therapy with hemostimulants during the following two years may be avoided.
Adult↗
[Use of the leukocyte mass in treatment of patients with suppurative lactation mastitis].
The application of leukocyte mass against the background of altered reactivity of the organism was followed by positive changes of clinical and immunological data by as early as the 2-3d days of the postoperative period. The level of antistaphylococcal immunity was preserved after completion of the course of leukotransfusions. It results in complete arrest of the inflammation in the mammary gland, excludes the development of recurrences and various pyo-septic complications. Leukotransfusion is not only a method of substitution therapy but also an effective immune-correcting factor.
Blood Transfusion↗
[Mixed gel precipitation in the analysis of monoclonal antibodies against determinants of the carcinoembryonic antigen].
Mixed gel precipitation technique was used in the study of 5 monoclonal antibodies (MA) to carcinoembryonic antigen (CEA). The method is based on specific inclusion of MA into the precipitate formed by the antigen and polyclonal antibodies to it. The test-system for the determination of nonspecific cross-reacting antigen (NCA) has demonstrated that MA 2D10 are directed to the determinant, common for CEA and NCA, whereas MA 3C12 and 2G10 give no reaction with NCA. The specificity of MA 192 and 35 correlated with the previously established one. Mixed gel precipitation technique is recommended for primary screening and study of MA specificity to any precipitating antigen.
Animals↗
[Purification of monoclonal antibodies using a staphylococcal reagent containing protein A].
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Animals↗
[Express evaluation of the severity of the course of lactation mastitis].
The authors consider that quantitative and qualitative characteristics of phagocytosis can be given with the help of lysosomal-cationic test (LCT) at different periods of acute purulent lactation mastitis. LCT gives prognosis of the course of the disease and a reliable estimation of the treatment efficiency.
Blood Bactericidal Activity↗
[Immunologic status and leukocyte function of patients with suppurative lactational mastitis].
In patients with a severe course of suppurative lactation mastitis there develops a relative deficiency in the cellular and humoral links of immunity. The compensatory activation of factors of nonspecific antiinflammatory resistance of the organism fails to exert a substantial positive effect on the development of the destructive process in these patients. On the basis of the data obtained, in order to improve results of the surgical treatment of patients with suppurative lactation mastitis means of the cellular anti-infection therapy, transfusions of donor leukocyte masses in particular, must be included in the complex of curative measures in the postoperative period.
Antibody Formation↗
Cross-reactivity of cytotoxic T-lymphocyte antigen-binding receptors immune to antigens of the H-2 system.
Anti-B10 D2 (anti-KbDb) cytotoxic T lymphocytes (CTL) and monospecific anti-Kk and anti-Dd CTL, separated from their mixture in anti-A B10 lymphocytes and concentrated with the absorption-elution technique on the macrophage monolayers of various genotypes, gave rise to cross-lysis of third-party target cells. Anti-B6 R101 (anti-Kb) CTL, which display a cross-lysis of mutant Kbd (M505) and Kba (Hzl) target cells, were separated into two fractions, one of them being able and the other unable to adhere to the cell monolayer of the particular mutant. The fraction of anti-Kb CTL adherent to Kba antigen was isolated by elution from the Hzl cell monolayer. These anti-Kba CTL and CTL eluted from the B6 cell monolayer displayed a considerably higher cytotoxic effect on B6 than on Hzl target cells. This difference was not due to lower sensitivity of Hzl targets to lysis. The findings indicate that anti-Kb CTL are homogeneous in the specificity of their receptors. The H-2 molecule is presumed to bear a single CTL determinant only, which induces a CTL clone or a group of clones homogeneous in specificity but heterogeneous in the affinity of its receptors.
Animals↗
[Cross reactions in the H-2 system of cytotoxic T-lymphocytes concentrated by the adsorption-elution method on target cell monolayers].
Cytotoxic T-lymphocytes (CTL) obtained by in vivo immunization were enriched by the absorption-elution technique, using the relevant allogeneic target cell (TC) monolayers. After the separation of C57BL anti-A (anti-KkDd) lymphocytes into anti-Kk and anti-Dd subpopulations they displayed cross killing cytotoxic effect on H-2d and H-2kTC, respectively. B10D2 anti-B10 (anti-KbDd) lymphocytes cross reacted to H-2a and H-2q TC. The results are discussed in the light of heterogeneity of CTL clones or their receptors.
Alleles↗
[Importance of immunotherapy in the management of patients with acute staphylococcal lactation mastitis].
Failure of antibiotic therapy against the background of sharply decreased defensive forces of the organism in the postpartum period made it necessary to perform a comparative clinical evaluation of the effect of homologous and heterologous gamma globulins. It has been established that treatment with these drugs results in normalization of clinical and immunological signs which are retained till the complete recovery of patients.
Acute Disease↗
[Use of heterologous antistaphylococcal gamma-globulin in treating suppurative mastitis].
The effectiveness of heterologic antistaphylococcal gammaglobulin was studied in the clinic in 67 cases of acute lactogenous mastitis. The activity of the preparation was 200 A. E/ml. The Bezredko method of injection was used--l ml once a day during 5 days. The treatment resulted in the decrease of intoxication, purification of the surgical wound, normalization on non-specific protection factors, rise of antistaphylococcal antitoxin titers and decrease of autosensitization phenomena.
Animals↗
[Immune lymphocyte inhibition of activation of DNA synthesis in mixed cultures of normal lymphocytes in vitro].
Substitution of normal lymphocytes by immune ones as responding cells in the "one-way" mixed lymphocyte culture (MLC) results in inhibition of the DNA synthesis. This phenomenon is reproduced irrespective of the mouse strains used, immunization method (in vivo or in vitro) and various MLC techniques (macro- or microcultures, the presence of mercaptoethanol in the medium, control variants). Unlike the cytotoxic effect of immune lymphocytes showing a sharp peak on the 8th day after in vivo immunization, inhibition of the DNA synthesis activation in MLC occurred as the second day, and persisted for several weeks after immunization; this inhibition was reproduced on addition of a small fraction (5 to 10%) of immune lymphocytes to normal ones.
Animals↗
[Use of peritoneal macrophages labeled with Cr51 and cultivated in vitro as target cells for quantitative assessment of the cytotoxic activity of immune T-lymphocytes].
Peritoneal macrophages previously labeled in a suspension with 51Cr and grown in the form of a discontinuous monolayer on glass, pretreated with poly-l-lysin were used as target cells. This permitted to estimate the cytotoxic activity of immune lymphocytes by 51Cr release for the period of 4 and 20 hours of incubation with the target. 51Cr release from such a target incubated with normal lymphocytes for 20 hours did not exceed 10-20% of the maximum release. Application of a 2% solution of sodium dodecylsulfate ensured a 100 per cent solubilization of labeled macrophages growing on the glass surface, and permitted the cytotoxic effect to be determined by measuring the label remaining in the intact cells.
Animals↗
[Interaction of anti-Kb and anti-Dd effector lymphocytes with target cells of mouse mutant haplotypes].
The magnitude of the cytotoxic effect (CE) of immune lymphocytes R101 anti-Kb (C57BL/6) and R101 anti-Dd (B10.D2) upon target cells (TC) of mutants Kba (Hzl) and Kbd (M505), as well as Dda (M504) was considerably less than that on the TC of the original C57BL/6 and B10.D2 strains, respectively. A decrease of the CE on the H (zl) and M504 TC was more prominent than that on the M505 TC. "Monospecific" lymphocytes anti-Kb and anti-Dd were separated into two distinct subpopulations by absorption on the monolayers of macrophages from the mutant strains of mice. One of the subpopulations reacted with the TC of the original strain only, whereas the other one - with the TC of both the original and mutant strains.
Alleles↗
[Abdomino-mediastinal lipomas].
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Abdominal Neoplasms↗
[Cyclic nucleotides in the ontogeny of the chick thymus].
The dynamics of cAMP and cGMP content of the thymus homogenate from developing chick embryos and chickens was studied during ante- and postnatal development. Changes in the content of cyclic nucleotides bear an oscillatory pattern. At the early stages of embryogenesis (9 to 11 days of incubation) the content of cyclic nucleotides was low and gradually increased by 13 days of incubation. As the development proceeded, the quantitative and qualitative rearrangement of the thymus cellular composition reflected in changes in the content of cyclic nucleotides. At the same time the curves of cyclic nucleotide content became antiphasic. These reciprocal cAMP to cGMP ratios might reflect the cyclic and synchronous reproduction and functional development of the main bulk of the thymus cellular elements. The maximum content of cAMP and the minimum content of cGMP were recorded on the 17th day of embryogenesis.
Animals↗