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A V Bolobova

Publications and source records attributed to A V Bolobova.

3 recordsLinked to original sources

The activation of cellulases from different sources by actin.

Actin has been shown to be a potent activator of the enzymatic hydrolysis of cellulose, its addition to the reaction mixture leading to an increase in the hydrolysis rate of up to 6-7 fold. The action of actin is directed primarily towards endoglucanases of cellulase complexes. The degree of activation varies in relation to the cellulases from different microbial sources. The activation of the enzymatic hydrolysis of an insoluble cellulose by actin does not affect the Michaelis constant but increases the maximum velocity of the reaction. Increasing the actin concentration leads to a linear increase in the activation effect which indicates a rather poor binding of actin with the cellulases under study.

Actins

[Rapid method for determining cellobiase activity].

A simple and rapid method for determining the cellobiase activity in purified enzyme preparations was developed. It is based on a series of consecutive enzymatic reactions, i. e. hydrolysis of cellobiose by cellobiase, oxidation of the forming glucose by glucose oxidase, and formation of a dyed product under peroxidase action in the same reaction system. The dyed product is recorded spectrophotometrically at 460 nm. One measurement takes from 2-3 to 7-10 min depending on a particular method of the activity determining. The reagent which is used for the activity determining can be obtained in the yophylized form and used repeatedly. The relative deviation of the method is 5-7%.

Aspergillus