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Biomedical subjects

A V Datsenko

Publications and source records attributed to A V Datsenko.

11 recordsLinked to original sources
PubMed1989

[Effect of lowered relative humidity of the air on the accumulation of plutonium-239 in the body and its biological effectiveness].

The experiments on rats show that the 4-month elevated air dryness has no impact on body accumulation of 239Pu but causes more distinct forms of bronchitis and interstitial pneumonia combined with the strain in medullary hemopoiesis and reactivity intensification of pulmonary and B-system immunity. Under prolonged effect of both factors a breakdown in fully adequate immune response can occur.

Air Pollutants↗
PubMed1986

[Automatic image analysis in the study of the microcirculatory bed].

The authors discuss the possibility of efficient morphometric analysis of capillary architectonics and pathological structural changes in microcirculatory bed with computer systems of image analysis using selective histological staining of microvessels. It is suggested that a histochemical method of alkaline phosphatase identification is used for cerebral, cardiac, adrenal, hepatic, splenal, gastrointestinal, sceletal muscle, testes, urine bladder, serosae and other rat organ capillaries computerized analysis. It is recommended to impregnate the microcirculatory bed of bigger omentum of large laboratory animals according to V. Kupriyanov's method, and to stain the labrocytes of the perivascular loose connective tissue of serious membranes in large laboratory animals with alcohol solution of toluidine blue.

Animals↗
PubMed1986

[Tissue basophils in thermal damage to the skin in an experiment (morphometric analysis and statistical modelling)].

By means of quantitative morphological analysis tissue basophils (TB) (mast cells) in the derm and in the subcutaneous loose connective tissue have been studied at various degree of thermal lesion of the skin. Average diameter, amount of the cells per 0.1 mm2 of the slice area and distance between them serve as criteria for estimation of the TB functional activity. Certain differences have been revealed in the TB state at a weak and severe thermal lesion of the skin. At a threshold thermal action, metabolic processes in the TB become active. Burns of the skin of the II and IIIa degree result in an increased discharge of the granular component from cytoplasm and often in death of the cells. By means of multiple linear regression equations certain dependence of the degree of the thermal skin lesion on the state of the TB population in various dermal layers and in the subcutaneous loose connective tissue is stated.

Animals↗
PubMed1986

[Multivariate statistical analysis of tissue basophils in acute radiation sickness].

By means of mark scale estimation method, discriminant and factor analyses, changes in integral indices on the state of mast cells in the rat mesentery have been investigated in dynamics of medullary, intestinal and cerebral forms of an acute radiation sickness. The integral indices are calculated basing on the morphometric parameters of the cells, that are obtained after the histological preparations are treated in a special automatic system for analysing images. At the medullary form of the acute radiation sickness, the greatest structural rearrangements of the mast cells take place during the first hours after the radiation, as well as during the climax of the disease, at the intestinal form, the analogous changes are revealed 1-3 days after the effect, and at the cerebral form--3 h after the radiation. The integral indices, calculated by means of the three methods, are well correlated with each other.

Acute Disease↗
PubMed1985

[Pathology of the rat mesenteric lymphatic bed in acute radiation sickness].

The authors have shown that in the medullary, intestinal and cerebral forms of acute radiation disease pathological changes of the lymphatic microvessels manifested themselves in the form of lymphostasis at the climax of disease in the medullary form of acute radiation disease and at the climax and in the terminal period in the intestinal form. A characteristic feature of the lymphatic channel involvement in the medullary form of acute radiation disease was a large number of erythrocytes in the lumen of the lymphatic vessels at the climax of disease. No changes of the mesenteric lymphatic channel of the rats were noted in the cerebral form. On the other hand, the presence of a large number of newly formed capillaries in the young rats at the climax of disease indicate the persistence of the proliferative ability of the lymphatic microvessel endothelium and its significant radioresistance.

Acute Disease↗
PubMed1984

[Changes in the microcirculatory bed of the mesentery in the rat in the intestinal form of acute radiation disease (experimental morphologic study)].

Total mesentery preparations of the rat after single total irradiation at the dose levels of 15 and 30 Gy were examined by V. V. Kupriyanov 's method and staining by N.E. Yarigin and S.V. Panchenko . Monotonous pathomorphological changes characteristic of microcirculation disorders were revealed such as blood overdistribution into the venous part of microvasculature prestasis , stasis and lymphostasis. Apart from these qualitative alterations, morphometry of certain parts of microvasculature was performed and some indexes and ratios were calculated. Changes of quantitative parameters were as follows: increase of the capillaries, postcapillaries and venules diameter, increase of their surface and volume, increase of diameter of pericapillary diffusion zone; decrease of the correlation ratio between total arteriolar influx and total venular blood passage within a mesentery segment; decrease of the calculated linear speed of blood in capillaries, postcapillaries and venulas .

Animals↗
PubMedDate not supplied

[Morphologic study of tissue basophils of the skin in rats after acute local irradiation].

A comparative morphological study was made of tissue basophils (TB) of rat skin at remote times (40, 70, 140, and 360 days) following single local exposure to soft and hard X-radiation with the doses at the skin surface of 15, 20, and 30 Gy. The TB response was maximum in the subepidermal and supramuscular layers of friable connective tissue. Changes in a median TB diameter were of a phase nature and a function of radiation dose absorbed in the skin layers under study.

Acute Disease↗
PubMedDate not supplied

[Changes in labrocytes during acute radiation sickness (morphometric study)].

Changes in the morphometric parameters of rat mast cells during acute radiation sickness have been studied. The most significant deviation of the quantitative indices of mast cells from the control values were noted at the height of the bone-marrow, at the terminal stage of the intestinal, and during the first few hours of the cerebral forms of acute radiation sickness.

Acute Disease↗