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Biomedical subjects

A V Fokin

Publications and source records attributed to A V Fokin.

12 recordsLinked to original sources
PubMed2002

Temperature evolution of sodium nitrite structure in a restricted geometry.

The NaNO2 nanocomposite ferroelectric material in porous glass was studied by neutron diffraction. For the first time, the details of the crystal structure including positions and anisotropic thermal parameters were determined for the solid material, embedded in a porous matrix, in ferro- and paraelectric phases. It is demonstrated that in the ferroelectric phase the structure is consistent with bulk data, but above transition temperature the giant growth of amplitudes of thermal vibrations is observed, resulting in the formation of a "premelted state." Such a conclusion is in good agreement with the results of dielectric measurements published earlier.

Journal Article↗
PubMed2001

Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide.

The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment.

Antibodies, Monoclonal↗
PubMed2000

[Spatial structure of a Fab-fragment of a monoclonal antibody to human interleukin-2 in two crystalline forms at a resolution of 2.2 and 2.9 angstroms].

The three-dimensional structure of the antigen-binding fragment of a monoclonal antibody to human interleukin-2 was determined in two crystal forms by the X-ray method of molecular replacement at 2.2 and 2.9 A resolutions. The spatial structure of the protein and the stereochemistry of its antigen-binding site were analyzed.

Amino Acid Sequence↗
PubMed1986

[Hemosorption detoxication in combined therapy of postoperative diffuse peritonitis in cancer patients].

Hemosorption was used in complex treatment of postoperative diffuse peritonitis in 69 cancer patients. A pronounced detoxicating effect manifested itself in removal of toxic metabolites from blood matched by an improvement in liver, kidney and cardiovascular function and blood rheologic properties. As a result, lethality rate fell to as low as 69.6% versus 88% in a group of 73 patients in whom the procedure was not used.

Abdominal Neoplasms↗
PubMed1986

[Preoperative hemosorption in cancer patients with mechanical jaundice].

The study was concerned with postoperative course and dynamics of biochemical indexes for liver function and degree of intoxication in 47 cancer patients suffering mechanical jaundice. The patients received hemosorption preoperatively. Hemosorption was found to significantly increase the efficacy of detoxifying therapy and to contribute to prevention of postoperative complications and lethality.

Cholestasis↗
PubMedDate not supplied

[The effect of hemosorption on the intensity of spontaneous chemiluminescence of the blood].

1 day after intensive irradiation of cancer patients chemiluminescent (CL) signal of blood sera is considerably enhanced. Hemoperfusion (HP) decreases it by 62%, with the following slight increase in CL intensity. 42-47% increase in electrokinetic erythrocyte potential registered in patients after HP, as well as a certain decrease in blood viscosity may be considered as an indirect explanation of the mechanism of triboluminescence of human blood during HP.

Adult↗