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Biomedical subjects

A V Kargapolov

Publications and source records attributed to A V Kargapolov.

At least 19 recordsLinked to original sources
PubMed1994

[Change in phospholipid content in platelets, immunocompetent cells and myometrial tissue in patients with internal endometriosis].

A method of flow horizontal chromatography has been developed permitting investigation of blood and tissue cellular phospholipids ruling out lipid peroxidation effects on cellular membranes. Phospholipid levels of blood and myometrial tissue cells were measured by this method in 67 patients with histologically verified internal endometriosis. Phospholipid and phosphatidyl inosite levels in platelets and immunocompetent cells of these patients reliably differed from those in healthy women. Phosphatidyl inosite levels of heterotopic endometrial tissue was increased by 1.4 times vs. the norm on an average. In parallel with this, a reliable change of phosphatidyl cholines and inosites levels in endometriosis foci were detected as against their levels in intact tissue. The authors suggest a method for the diagnosis of internal endometriosis by phosphatidyl inosite levels in blood lymphocytes.

Adult↗
PubMed1993

[Changes in the content of phospholipids and their metabolic products in the blood and tissues of patients with endometriosis interna and cancer of the corpus uteri].

Levels of phospholipids, phosphoinosites, phosphatidylinosite-3-phosphates, diacylglycerols, arachidonic acid, prostaglandins E, F, J, and thromboxane A2 in patients with endometriosis and cancer of the corpus uteri were found to differ from those in healthy women. Biochemical parameters of whole blood and myometrial tissue of endometriosis patients differ significantly from those of patients with corpus uteri cancer. The authors suggest that reduction of phosphatidylinosite and phosphatidulinosite-3-phosphate levels in whole blood and their parallel increase in myometrial tissue are conducive to cellular proliferation and malignant degeneration.

Adult↗
PubMed1993

[The dynamics of the rapid changes in the phospholipids of the whole blood and blood cells in patients with endometriosis interna].

Time course of rapid changes in blood phospholipids and blood cells was studied in 44 patients with endometriosis. Rapid reversible blood phospholipid changes were observed when these patients' red cells and lymphocytes were incubated with calcium chloride. Time course of rapid changes in phosphoinosites, sphyngomyelines, phosphatidylserines, phosphlatidylethanolamines of patients' red and white blood cells, whole blood, and myometrial tissue differed significantly from that in healthy women. The authors suppose that cellular proliferation processes in endometriosis are connected with phospholipid homeostasis disorders in blood cells and tissue.

Adult↗
PubMed1992

[Characteristics of the dynamics of rapid changes in blood phosphoinositides in patients with alcoholic heart diseases].

A total of 42 patients who had been abusing alcoholic beverages during different periods and 12 healthy individuals were examined. Their intracardiac hemodynamic parameters were evaluated by echocardiography and rapid changes in inositol-containing blood lipids were determined by thin-layer flow chromatography. The patients showed significantly reduced contractility of the left ventricle, its wall hypertrophy and greater cavity dimensions. These abnormalities were more pronounced in patients with an over 10-year history. Biochemical studies revealed a time course of rapid changes in inositol-containing lipids in patients, which showed more fluctuations in the content of phosphoinositides and their lower baseline and final levels and differed from that in healthy persons. It was stressed that the changes in inositol-containing lipids were also related to the duration of alcohol abuse and they reflected the mechanisms of the toxic action of ethanol on the myocardium, which might be useful in the assessment of the severity of cardiovascular events in alcoholism.

Adult↗
PubMed1991

[The dynamics of the blood level of phosphatidylinositol in patients with myocardial infarction].

Phospholipids, particularly inositol-containing ones, that serve as secondary messengers and bioregulators of fundamental biochemical processes, essentially contribute to the origination of cardiovascular diseases. These compounds are highly labile and the data on their levels in the blood and its components in various cardiovascular conditions are contradictory. This may be explained by the fact that phosphatidylinositol levels change immediately after blood collection. Therefore the authors found it interesting to investigate the time course of this phospholipid levels, that may to a certain measure reflect the pattern of the metabolic disorders present in this case. They suggest a method of multiple measurements of phosphatidylinositol levels, carried out in short intervals, this yielding additional data on the function of biologic membranes, that may be useful in the diagnosis of coronary disease and assessment of the efficacy of treatment of this condition.

Adult↗
PubMed1991

[The dynamic change in the content of total lipids in myocardial infarct patients].

The dynamics of the content of total lipids (cholesterol and cholesterol esters) was studied in patients with acute large-focal uncomplicated myocardial infarction in different disease periods. Rapid quantitative changes that occur in the content of cholesterol and its esters in the test groups were established to be characterized by marked specificity and by the form of its own in different disease periods (in the acute period--days 2-5 and on day 15--the health status stabilization). It is advisable that the characteristics of the dynamics of rapid reversible changes in the content of total lipids may be used for estimating the status gravity of patients with uncomplicated large-focal myocardial infarction.

Adult↗
PubMed1990

[Changes in phosphatidylinositol levels in the blood and its components in patients with leukemia].

Continuous thin-layer chromatography was used in the assay of phosphatidylinositol (PI) content in the blood and its fragments. A significant reduction in the level of these compounds was recorded in leukemia patients as compared to that in normal donors and anemia patients. The reduction of PI level in the blood may evidence that the enzymatic systems of the blood cells have partially lost their capacity for maintaining the homeostasis of these compounds. The treatment of leukemia patients with lanvis, myleran and chlorambucil improved the general condition of the patients and normalized their clinico-laboratory parameters. A significant rise of PI level in the blood and its fragments as compared to the levels before the treatment was observed in these patients. The data obtained could be used in clinical practice for complex diagnosis of leukemia as well as for the patient follow-up during the treatment with varying drugs.

Adult↗
PubMed1990

[Changes in the level of tightly-bound mitochondrial phospholipids induced by exogenous factors].

The effects of some parameters of the incubation medium (tonicity, H+ and Pi concentration) on the phospholipid content in rat mitochondrial extracts prepared by normal extraction and by extraction with strongly acidified solvent mixtures, were studied. It was found that after normal extraction with a chloroform-methanol mixture, 8% to 25% of mitochondrial phospholipids can be additionally extracted by acidified solvent mixtures. The concentration of weakly extractable lipids depends on the tonicity and ionic composition of the incubation medium. It was suggested that there exist in mitochondria two lipid pools, a loosely and a tightly-bound ones; the equilibrium between these pools is effectively regulated by medium tonicity and other external factors.

Animals↗
PubMed1990

[Blood level of phosphatidylinositols as a criterion of diagnosis and control of the effectiveness of surgical treatment in gastric cancer].

In patients with gastric cancer, the content of phosphatidylinositols in the blood was significantly decreased, in average 2-fold, as compared with their level in donors. After surgical treatment in 36 patients, these indices increased and achieved a maximum value in a year. In these patients, the general state improved, there were no disease recurrences. In 44 patients after the operation, the content of phosphatidylinositols remained low, or increased insignificantly as compared with their level before the operation. In this group, 16 patients died, 10 developed a disease recurrency.

Humans↗
PubMed1990

[The phosphatidylinositol content of the blood in cancer patients].

Phosphatidyl inosite level was measured in the whole blood, plasma, erythrocytes and platelets of patients with cancer of various sites (270), benign tumors (50), and hemoblastoses and anemia (60 cases). Phosphatidyl inosite levels in all the substrates in cancer patients, on the average, proved half the normal one while, in the benign tumor group, they only tended to decrease. The levels did not depend upon blood count.

Blood Platelets↗
PubMed1981

[Study of lipid composition of mitochondrial and endoplasmic membranes by horizontal flow chromatography].

A new modification of a highly sensitive horizontal flow chromatography procedure for the study of lipid composition of intracellular membranes is proposed. This modification employs application of thin layers of homogeneous silicagel particles by precipitation from diluted water suspensions in a cell of original construction, which allows to obtain by a single analysis distinct lipid fractions having similar chromatographic mobility. Using this modification, the major forms of lysophosphatides, i. e. lysophosphatidylcholines, lysophosphatidylethanolamines, lysocardiolipids, lysophosphatidic acids, lysophosphatidylserines, etc., and their diacyl analogs were isolated from mitochondrial and reticulum membranes and identified. The contents of mono-, di- and triglycerides, free fatty acids, cholesterol and its esters were determined.

Animals↗
PubMed1979

[Changes in the phospholipid content of intact mitochondria under mitochondrial swelling in hypotonic sucrose solutions].

Mitochondrial swelling in hypotonic sucrose solutions is accompanied by a decrease of the amount of phosphatidyl ethanolamines, cardiolipids and their lyso-derivatives. An addition of dicaine (inhibitor of phospholipase) to the incubation medium results in a considerable decrease of mitochondrial swelling and prevents the decay of phosphatidyl ethanolamines and cardiolipids. This effect is due to the inhibition of corresponding phospholipases. The data obtained suggest that the passive mitochondrial swelling is accompanied by activation of endogenous phospholipases and by a hydrolysis of cardiolipids, phosphatidyl ethanolamines and their lyso-derivatives.

Animals↗
PubMed1978

[Effects of uncouplers on the phospholipid composition of mitochondria].

A decrease in the level of lysocardiolipin and lysophosphatidylethanolamine in mitochondria induced by chlorophenacyl and 2,4-dinitrophenol was demonstrated. The effects of the uncouplers were especially well-pronounced under conditions of mitochondrial swelling. It was found that the phospholipase inhibitor nupercain completely reverses the effects. The data obtained suggest that 2,4-dinitrophenol and alkylating compounds increase the phospholipase activity of mitochondria under given experimental conditions.

Animals↗