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A V Kher

Publications and source records attributed to A V Kher.

At least 19 recordsLinked to original sources
PubMed2000

Efficacy of a modified Ultra Fast Papanicolaou (UFP) stain for breast aspirates.

The chief limitation of Ultra Fast Papanicolaou (UFP) stain, suggested by Yang and Alvarez (1995), is that, Richard Allan Haematoxylin (RA-H) and Richard Allan Cytostain (RA-C), used in the staining procedure are not universally available. It has not been established so far, whether, changes in the reagents and their proportions depending on the local availability, influence the performance of the stain. The objective of this study was to assess the feasibility and applicability of a modified UFP stain to suit our local laboratory supplies of chemical dyes, as applied to the permanent smears prepared from Fine Needle Aspiration (FNA) of breast lumps. In the present study, smears from FNA from 100 breast lumps were stained by the modified UFP stain. The modification consisted of following two changes: use of Gill's Haematoxylin instead of RAH and omission of Orange G from cytostain. Eighty Six breast aspirates were adequate for interpretation. Smears showed transparent cells with crisp nuclear features, equal to and even better than the conventional Papanicolaou stain, in a blood free background. There was an increase in total staining time by 40 seconds. We recommend the use of this modified UFP stain, only if similar reagents are being used in other laboratories. Otherwise situation specific modifications may be needed. If the UFP stain is to be used for tissues where the chances of cytoplasmic keratinization are negligible, then the use of Orange G component of the stain may become redundant.

Biopsy, Needle↗
PubMedDate not supplied

Evaluation of fine needle capillary sampling in superficial and deep-seated lesions. An analysis of 670 cases.

OBJECTIVE: To study the efficacy and merits of a simplified cytologic technique of fine needle capillary (FNC) sampling in 670 cases. STUDY DESIGN: FNC sampling was tested on a consecutive series of 600 superficial palpable masses and 70 deep-seated lesions during the period January 1993-September 1993. Among superficial masses, sampling was done on 380 lymph nodes, 88 breast lesions, 56 thyroids, 12 salivary glands and 64 miscellaneous sites. Fifty-five lung lesions were sampled using 8 computed tomography, while 15 abdominal masses were sampled using ultrasonography. Cell samples were assessed as diagnostically adequate or inadequate. RESULTS: The diagnostic adequacy in lymph node, breast and thyroid lesions was 95%, 91.07% and 90.09%, respectively. The diagnostic adequacy for deep-seated lesions was 91.42%. CONCLUSION: The procedure gives a better perception of tumor consistency and control of the hand. FNC resulted in a high yield of good-quality material with retention of cellular architecture. It is recommended that the operator become familiar with the macroscopic slide appearance of a cellular smear. Doubts persist with respect to fibrotic and cystic lesions.

Adult↗
PubMedDate not supplied

Florid melanocytic colonization in a metastasis of breast carcinoma. A case report.

BACKGROUND: Melanocytic colonization in breast carcinoma with skin invasion is an unusual entity. Very rarely does florid colonization by melanocytes simulate melanoma. This phenomenon requires recognition. The cytomorphology of this lesion is reported below for the first time. CASE: A 45-year-female operated on for breast carcinoma developed a recurrent nodule on the mastectomy scar and another circumscribed, depigmented nodule on the back. Fine needle aspiration cytology smears from the recurrent nodule showed features of lobular carcinoma of the breast, whereas the nodule on the back showed cytomorphologic features simulating melanoma along with cells simulating lobular carcinoma of the breast. CONCLUSION: We report for the first time the cytologic finding of melanocytic colonization in subcutaneous secondary breast carcinoma.

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