Biomedical subjects

A V Pavlov

Publications and source records attributed to A V Pavlov.

75 records · Page 5Linked to original sources

PubMedDate not supplied

[The mitotic origin of binucleated cells in regenerating endothelium].

Regeneration of endothelium after the local cryodestruction of the aortic wall in rats was studied using quantitative methods (determination of the pool of proliferating cells after multiple injections of 3H-thymidine, of the mitotic index and proportion of binucleated cells, and cytophotometry of DNA content in cell nuclei). It is demonstrated that binucleate endotheliocytes are formed as the result of acytokinetic mitosis. According to our calculations, this mechanism completely provides for the increase in the proportion of binucleated cells observed in the regenerating endothelium on days from 2 to 6 after the operation.

Animals↗

PubMedDate not supplied

[The use of synthetic peptides for detection of antibodies against foot-and-mouth disease virus in the blood from convalescent animals].

Peptides were synthesized, which, according to theoretical analysis of the antigenic structure of protein VP1 of foot-and-mouth disease (FMD) virus types A, 0, and Asia 1, corresponded to potential immunodominant protein sites. Activities of the peptides were studied by solid-phase indirect radioimmunoassay on polyethylene film with purified immunoglobulins against intact FMD virus. Virtually no cross reactions were observed. Blood sera of cattle convalescent after FMD were tested with the FMD virus and peptides containing VP1 fragments 141-160 (A22 No. 550), 140-160 (O1 No. 194), and 140-153 (Asia 1 No. 48). The specificity of interactions between the sera and peptides and the virus was uniform, this permitting the identification of the virus type which caused the disease.

Amino Acid Sequence↗

PubMedDate not supplied

[Cytologic mechanisms of postnatal growth of adrenal chromaffin tissue].

We have studied the contribution of proliferation and hypertrophy of glandular cells to the ontogenetic growth of adrenal chromaffin tissue using several methods (organometry, cytometry, cytophotometric quantitation of DNA in the nuclei, radioautographic analysis of 3H-thymidine incorporation, calculation of the mitotic index and proportion of binuclear cells, as well as stereological analysis). Mitotic division of diploid glandular cells is the main cellular mechanism of postnatal growth of chromaffinocytes. It is most prominent during the first 2 weeks of life and is maintained at a rather high level throughout the life of animals (the daily proliferative pool in 6-month-old and 30-month-old rats equals 0.3%). Development of cellular hypertrophy has been noted during the first 6 months after birth. The population of chromaffinocytes throughout life is practically diploid; the proportion of tetraploid (binuclear) cells does not exceed 1-1.5%. The growth of adrenal chromaffin tissue during the first month of life is generally supported by hyperplasia and hypertrophy of norepinephrocytes and later of epinephrocytes. The contribution of cell proliferation and hypertrophy to postnatal growth of each subpopulation appears to be equal.

Animals↗