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A V Provotorov

Publications and source records attributed to A V Provotorov.

9 recordsLinked to original sources
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[The formation and repair of DNA breaks in subpopulations of irradiated rat bone marrow cells].

Using the method of viscosimetry of alkaline lysates it was shown that the saturable DNA repair system was present in rat bone marrow cells which was described by a complex kinetics. Isokinetic fractionation in a sucrose and dextran gradient permitted to isolate three fractions differing in the ratio between differentiated cells and cells capable of division. The estimation of the postirradiation DNA repair kinetics in these cells revealed a comparatively high level of repair in normocytes which confirmed the presence of the relationship between the level of DNA repair in cells and their proliferative potency.

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[The rate factor in DNA sedimentation studies].

Zimm theory was used to study the effect of the rate of centrifugation on the coefficient and the profile of sedimentation of a polydispersed single-stranded DNA. There was a good agreement between the calculated and experimental DNA sedimentograms in alkaline lysates of irradiated rat bone marrow cells. This approach permited to take the rate factor into consideration on the basis of the parameters necessary for the work of the rotor and the gradient. Simple formulae were obtained connecting the molecular mass of DNA with the rate and the time necessary for its centrifugation. Possible consequences of not taking the rate factor into account in centrifuging the polymer DNA molecules are discussed.

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[Sedimentation of alkaline lysates of irradiated rat bone marrow cells].

A method has been developed for registration of sedimentation diagrams of alkaline lysates of mammalian cells based on measuring UV-absorption of fractions of linear sucrose gradient during its passage down the flow of UV-cord. DNA sedimentation in alkaline lysates of irradiated bone marrow cells of rats was analyzed. The ability of these cells to repair single-strand breaks during the postirradiation incubation in buffer at 37 degrees C was demonstrated. The proposed method could be applied for screening the compounds affecting the damage and repair of DNA in a cell.

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[Induction of double-strand DNA breaks in rat bone marrow cells by X-radiation].

The method of sedimentation in a neutral sucrose gradient was used to study double-stranded DNA in a total population of rat bone marrow cells. As a result of cell lysis in neutral conditions the fragments of double--stranded DNA were formed having the molecular mass of (3 +/- 0.3) X 10(9)D. A study was made of the dynamics of accumulation of DNA double-strand breaks after irradiation of a cell suspension. It was shown that the yield of double-strand breaks and the ratio between single- and double--strand breaks in bone marrow cells were similar to those of cultured L5178Y cells.

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[Formation and repair of DNA breaks in bone marrow cells of the irradiated rat].

The method of sedimentation was used to study the induction and repair of DNA breaks in irradiated rat bone marrow cells. It was shown that a total population of bone marrow cells did not differ from that of cultured L5178Y cells with regard to the yield of single-strand breaks and the ratio between double- and single-strand breaks. The absence of double-strand breaks and incomplete repair of single-strand breaks (70%) were noted with doses ranging from 20 to 100 Gy. The quantity of unrepaired single-strand breaks did not correlate with that of double-strand breaks, induced by the same radiation doses. This is indicative of the capacity of bone marrow cells for repair in a short-term culture.

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