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Biomedical subjects

A V Ritter

Publications and source records attributed to A V Ritter.

At least 19 recordsLinked to original sources
PubMed2001

Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen.

The purpose of this study was to evaluate the effects of phosphoric acid (PA) and a proprietary glutaraldehyde-HEMA aqueous solution (Gluma Desensitizer; GD) on dentin collagen. Specimens of demineralized bovine dentin collagen were treated with either 37% or 50% PA for 1 or 5 min. An additional set of specimens was treated with 37% PA for 1 min followed by GD for 1 min. All specimens were washed with distilled water, lyophilized. reduced with standardized NaB3H4, hydrolyzed with 6 M HCl and subjected to amino acid and cross-link analyses. The results demonstrated that the treatment of demineralized dentin with PA under the conditions tested did not significantly alter the collagen cross-links. The GD-treated samples showed reduction of free lysine (Lys) and hydroxylysine (Hyl) residues, as well as a decrease in the levels of collagen reducible cross-links. In addition, unidentified reducible compounds were detected by high-performance liquid chromatography (HPLC) analysis. These compounds may be derived from cross-links formed between GD-derived aldehyde and Lys/Hyl of collagen. The findings indicate that PA treatment does not significantly affect dentin collagen amino acid and cross-link composition, and that GD treatment affects dentin collagen amino acid and cross-link composition.

Amino Acids↗
PubMed2001

Four-year clinical evaluation of posterior resin-based composite restorations placed using the total-etch technique.

PURPOSE: To report the 4-year clinical performance of posterior resin-based composite restorations placed using the total-etch technique. MATERIALS AND METHODS: Over a period of 1 year, 726 restorations (248 molars, 478 premolars; 260 Class I, 466 Class II; 540 replacements, 186 primary decay) were placed on conservative preparations, using the incremental placement technique in a clinical environment. Baseline data were collected, and the restorations were evaluated after 4 years. Z100 and Scotchbond Multi-Purpose (SBMP) (3M Dental Products, St. Paul, Minnesota) were used as the restorative system. The criteria evaluated were color match, marginal adaptation, anatomic form, cavosurface marginal discoloration, axial contour, interproximal contact, secondary caries, postoperative sensitivity, and tooth vitality. RESULTS: At baseline, 24% of the teeth restored presented postoperative sensitivity; 86% of the sensitive teeth were from the replacement group. At 4 years, all teeth were vital to cold test. Eighteen restorations (2.5%) presented clinically detectable marginal fracture. The shade was acceptable in all restorations. Cavosurface marginal discoloration was observed in 47 restorations (6.5% bravo scores). Axial contour, interproximal contact, and marginal adaptation received 100% alfa scores. No secondary caries was diagnosed in any of the teeth examined. None of the examined restorations required replacement. CLINICAL SIGNIFICANCE: Under controlled clinical conditions, posterior resin-based composite restorations placed with the total-etch technique and restorative Z100/SBMP have the potential to present a high success rate at 4 years. None of the examined restorations required replacement, and there was no clinically detectable wear in any of the restorations. Simultaneous etching of enamel and dentin followed by the application of a resin adhesive can be considered an adequate modality of pulp protection in nonexposed tooth preparations.

Acid Etching, Dental↗
PubMed2001

Dental implants.

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Adult↗
PubMed2001

Composite bonding.

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Composite Resins↗
PubMed2001

Dentin bond strengths as a function of solvent and glutaraldehyde content.

PURPOSE: To evaluate the effect of the inclusion of glutaraldehyde on the shear bond strength of new ethanol-based adhesives (Gluma Comfort Bond & Desensitizer, GCBD and Gluma Comfort Bond, GCB) versus acetone-based Gluma One Bond (GOB) and a conventional three-step adhesive as a control. MATERIALS AND METHODS: Forty bovine incisors were mounted in acrylic, polished to 600-grit, and randomly assigned to four groups (n=10). Dentin was etched for 15 s using 35% phosphoric acid, rinsed, and lightly dried. After adhesive application and curing, Charisma composite was applied in a #5 gelatin capsule and light-cured. Specimens were loaded in shear using an Instron at 5 mm/min. Shear bond strengths were calculated by dividing failure load by bonded surface area. ANOVA showed a significant difference in means at P< 0.01, so Tukey's test was used for pairwise comparisons. RESULTS: Mean SBS (+/- SD, MPa) were: GOB: 8 (4.4)ab; GCB: 10 (4.4)ab; GCBD: 14 (6)bc; SBMP: 17.7 (6.4)c. Superscript letters indicate Tukey's homogeneous subsets (P< 0.05).

Acetone↗
PubMed2000

Influence of post placement in the fracture resistance of endodontically treated incisors veneered with direct composite.

STATEMENT OF PROBLEM: Veneer preparations are considered to weaken endodontically treated maxillary incisors. Prefabricated posts have been controversially indicated to reinforce endodontically treated teeth before final restoration. PURPOSE: This in vitro study evaluated whether (1) veneer preparation in enamel or in enamel/dentin weakens endodontically treated maxillary incisors, (2) bonding of direct composite veneer restores the original strength of the unprepared teeth, and (3) use of prefabricated metal posts increases fracture resistance of prepared and restored teeth. MATERIAL AND METHODS: Ninety extracted human maxillary central incisors were submitted to conventional root canal treatment. Specimens were randomly divided into 8 experimental groups (veneer preparation in enamel or dentin with/without post and with/without direct composite veneer restoration) and a control group (n = 10). Specimens were loaded to fracture, and the data were analyzed statistically. RESULTS: Statistical analysis revealed that a conservative veneer preparation does not significantly reduce maxillary incisors' fracture resistance. For prepared incisors, bonding of direct composite veneer restored their original strength, and the use of posts did not increase their fracture resistance. CONCLUSION: Conservative veneer preparations involving enamel and enamel/dentin did not significantly reduce the fracture resistance of endodontically treated maxillary incisors. In addition, restoration of the intraenamel preparations with direct composite resulted in teeth more resistant to fracture than teeth having restorations in dentin. The use of posts did not improve fracture resistance of endodontically treated maxillary incisors reduced and veneered with direct composite.

Analysis of Variance↗
PubMed2000

Effects of different re-wetting techniques on dentin shear bond strengths.

PURPOSE: For contemporary hydrophilic resin adhesive systems, bonding to dentin is improved if the substrate is maintained in a hydrated state following acid-etching. The purpose of this study was to compare the dentin shear bond strengths of two single-bottle adhesives (one acetone-based and one ethanol-based) applied under different etched-dentin conditions: dry, wet, or dry and re-wetted with different solutions. MATERIALS AND METHODS: Bovine incisors (N = 120) were mounted in acrylic, polished to 600-grit, and randomly assigned to 12 groups (n = 10). Dentin was etched for 15 seconds using 35% phosphoric acid, rinsed, and either blot-dried, air-dried, or air-dried and re-wetted with different solutions (distilled water, Gluma Desensitizer, Aqua-Prep, and 5% glutaraldehyde in water). Two adhesives (Single Bond and Prime & Bond NT) were applied to each of the surface conditions following manufacturers' instructions. After adhesive application and curing, composite was applied in a No. 5 gelatin capsule and light-cured. Specimens were loaded in shear, using an Instron at 5 mm per minute. Shear bond strengths were calculated by dividing the failure load by the bonded surface area. Data were subjected to analysis of variance (ANOVA) and a post hoc Tukey test. RESULTS: Mean shear bond strengths ranged from 12.5 to 26.6 MPa for Single Bond and from 5.6 to 14.7 MPa for Prime & Bond NT. Significant differences were found in both groups of materials (p < .001). The three highest mean bond strengths were obtained (in order) on dentin that was re-wetted with Gluma Desensitizer, re-wetted with Aqua-Prep, or never dried. Differences between these surface conditions were not statistically significant for either material.

Acetone↗
PubMed2000

Shear bond strengths of one-bottle adhesives to oil-contaminated enamel.

PURPOSE: To evaluate resin bond strength to enamel contaminated with handpiece oil. MATERIALS AND METHODS: Bovine teeth were randomly assigned to six groups of 20 teeth each for treatment with one of six different bonding systems (five one-bottle and one multibottle). For each system, 10 enamel specimens were contaminated with handpiece oil before acid-etching and 10 were contaminated after acid-etching. The enamel was etched for 15 seconds using 35% phosphoric acid. Following adhesive application, composite resin was bonded using a gelatin capsule technique. Shear bond strengths from the two contaminated groups were compared to bond strengths to uncontaminated enamel obtained from a previous study that was performed by the same group of investigators, using the same facility, materials, and methods. RESULTS: Two-way analysis of variance (ANOVA) showed that the factor "surface contamination" did not have a significant effect on bond strength (p > .542). The type of adhesive and the interaction of adhesive and surface contamination were significant (p < .0001 and p < .003, respectively). When oil was applied before etching, mean bond strengths ranged from 18.0 +/- 4.8 MPa for OptiBond SOLO (Kerr Corp., Orange, California) to 25.3 +/- 5.6 MPa for Tenure Quik with Fluoride (Den-Mat Corp., Santa Maria, California). With oil applied after etching, bond strengths ranged from 18.4 +/- 8.0 MPa for Tenure Quik with Fluoride to 27.4 +/- 5.4 MPa for Single Bond (3M Dental Products, St. Paul, Minnesota). For the same adhesive, comparing uncontaminated and "oil-before-etch" contaminated groups, the only statistically significant difference in bond strengths was for OptiBond SOLO: 21.8 +/- 4.0 MPa (uncontaminated) versus 18.0 +/- 4.8 MPa (oil before etch). Comparing uncontaminated and "oil-after-etch" groups, the only statistically significant difference was for Tenure: 24.5 +/- 5.7 MPa (uncontaminated) and 18.4 +/- 8.0 MPa (oil after etch).

Analysis of Variance↗
PubMed2000

Unique enamel phenotype associated with amelogenin gene (AMELX) codon 41 point mutation.

Different mutations in the amelogenin gene (AMELX) result in the markedly different enamel phenotypes that are collectively known as amelogenesis imperfecta (AI). We hypothesize that unique phenotypes result from specific genetic mutations. The purpose of this study was to characterize the enamel compositional and structural features associated with a specific AMELX mutation in three families with X-linked AI. We performed mutational analysis by amplifying AMELX exons and sequencing the products. Permanent and primary affected (N = 6) and normal (N = 3) teeth were collected and examined by light, scanning, and transmission electron microscopy. Enamel proteins were evaluated by immunolocalization of amelogenin and amino acid analysis. AI-affected individuals all shared a common AMELX point mutation (C to A change at codon 41). The dental phenotypic findings were remarkably consistent in all affected individuals. The AI enamel was opaque, with numerous prism defects or holes encompassing the entire prism width. Affected crystallites appeared more radiolucent and morphologically less uniform, compared with that of normal enamel. Immunogold labeling with anti-amelogenin antibodies localized amelogenin to the crystallites but not to the inter-crystalline spaces. No immunogold labeling was seen in normal enamel. There was an increased and amelogenin-like protein content in AI enamel (0.95%) compared with normal enamel (0.13%). We conclude that this codon 41 C to A missense point mutation, in a highly conserved region of the AMELX gene, results in a remarkably consistent phenotype.

Amelogenesis Imperfecta↗
PubMed2000

Medium-viscosity polyether impression material: a case report.

Polyethers are elastomeric materials commonly used for making precision impressions. They are hydrophilic, which facilitates the contact of the unset material with the intraoral tissue and the wetting of the impression by cast materials. The final hardness of this new impression material is reduced in an attempt to overcome rigidity of the set impression. A case is described involving the restoration of a fractured maxillary premolar and a single-phase impression technique using the new polyether impression material.

Bicuspid↗
PubMed1999

Ceramic restorations for posterior teeth: guidelines for the clinician.

UNLABELLED: Metal-free ceramic restorations are increasingly popular for restoring posterior teeth. These restorations are generated through a variety of techniques (e.g., CAD-CAM, copy-milling, heat-pressing, and firing). When appropriately indicated and made, ceramic inlays or onlays can be reliable and provide a highly serviceable restoration. The aim of this article is to review and present updated information regarding indications, restorative technique, and maintenance for this class of restorations, with emphasis to fired ceramic inlays or onlays. The information presented is based on 15 years of controlled clinical experience with this category of restorations. Clinical cases that represent some applications also are presented. CLINICAL SIGNIFICANCE: The fired ceramic inlay/onlay technique is presented as a viable option for the esthetic and adhesive restoration of posterior teeth.

Bicuspid↗
PubMed1999

Shear bond strengths of one-bottle adhesives to moist enamel.

PURPOSE: This study evaluated bond strengths of six one-bottle bonding agents and a control (primer plus unfilled resin) to moist enamel. MATERIALS AND METHODS: One-hundred and five bovine teeth were randomly assigned to seven groups of 15. Enamel was etched for 15 seconds with 35% phosphoric acid. Etched enamel was rinsed, and excess water was blotted with tissue paper. Following application of the adhesive, composite resin was bonded using a gelatin capsule technique. Shear bond strengths to enamel were determined using a universal testing machine (Instron Corp., Canton, Massachusetts). RESULTS: Mean bond strengths ranged from 21.9 MPa for OptiBond Solo (Kerr Corp., Orange, California) to 29.6 MPa for Prime & Bond 2.1 (Dentsply/Caulk, Milford, Delaware). Prime & Bond 2.1 had a significantly higher mean bond strength than the other adhesives. CLINICAL SIGNIFICANCE: The results of this study suggest that all of the one-bottle systems tested should provide clinically acceptable bonding to moist enamel.

Animals↗
PubMed1999

The effect of finishing and polishing on the decision to replace existing amalgam restorations.

OBJECTIVE: The purpose of this investigation was to evaluate the influence of finishing and polishing procedures on the decision to replace existing amalgam restorations. METHOD AND MATERIALS: Twenty Class I and Class II amalgam restorations, free from obvious defects, were selected in 6 patients. The restorations were photographed before and after being submitted to a standard finishing and polishing procedure. In the first phase, the preoperative slides were examined by 27 clinicians and senior dental students, who were instructed to inspect each restoration and answer a questionnaire indicating if and why the restoration needed to be replaced. Two weeks later, the postoperative slides were presented to the same examiners, who were asked to answer the same questionnaire as before. RESULTS: At the first phase, there were 236 decisions (44%) to replace existing amalgam restorations. Following the finishing and polishing procedures, 114 decisions (21%) were made to replace existing amalgam restorations. This difference was statistically significant. Secondary caries was the most common reason for replacement. CONCLUSION: The finishing and polishing procedure reversed the decision to replace old amalgam restorations.

Chi-Square Distribution↗