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Biomedical subjects

A V Romaniuk

Publications and source records attributed to A V Romaniuk.

6 recordsLinked to original sources
PubMed1995

[The protective effect of the nerve growth factor on sensory neurons in organotypic culture under the action of diphtheria toxin].

Diphtheria toxin (1.10(-1) - 1.10(-6) Lf/ml) was found to inhibit neurite extension in chick embryo dorsal root ganglia in vitro. If the nerve growth factor (60 ng/ml) was added with the toxin to culture media, the effect of diphtheria toxin was seen to decrease and the neurite outgrowth was compared with control. Protective effect of nerve growth factor estimated by the influence of diphtheria toxin may be used for new principles of diphtheria treatment.

Animals↗
PubMed1995

[The effect of veratridine on the release of neurotrophic factors in a nerve tissue culture].

A depolarising agent veratridine was shown to affect the level of neurotrophic substances in a combined culture of the newborn rats hippocampus and chick embryo spinal ganglia: the growth of sprouts of the ganglia's sensitive neurons was enhanced. The effect seems to be mediated by the veratridine effect on the hippocampal neurons. The role of an increased level of neurotrophic factors in an increase of neuronal activity, is discussed.

Animals↗
PubMed1988

[Comparative analysis of brain neurite-stimulating protein and nerve growth factor by using a culture of chick embryo sensory neurons].

The effect of brain neurite-stimulating protein (BNSP) on growth and differentiation of nerve cells was studied in cultured chick embryo neurons BNSP stimulated axon growth. Its chemical characteristics were different from those of neurotrophic factor (NGF). The effect of NGF antiserum was not blocked by the BNSP action. The neurite-stimulating effect of BNSP can be used for stimulation of regenerating processes in the nervous tissue.

Animals↗
PubMed1987

[Brain neurite-stimulating protein].

A protein possessing the neurite-stimulating activity in organotypic cultures of chicken embryo spinal ganglia was isolated and purified from bovine brain tissue. The isolation and purification procedures included acid extraction, ultrafiltration, preparative polyacrylamide gel electrophoresis and chromatography on heparin-Sepharose. The molecular weight of the protein is about 15000 Da. The neurite-stimulating activity of the purified protein manifests itself at a protein concentration of about 10(-9) M.

Animals↗
PubMed1986

[Effect of a cerebral neurite-stimulating protein on the morphogenesis of an organotypical culture of spinal ganglia].

Cationic protein with molecular mass of 15,000 kDa has been extracted from the hemispheres of the rat and bovine brain. Addition of the protein into nutritive medium of the organo-typical culture of the chick embryo spinal ganglia results in a considerable (by 2-2.5 times) increase of the growth zone in the explants. Neurite-stimulating effect of the protein is demonstrate dat concentration of 10 ng/ml. An intensive longitudinal growth of neurites is observed, increase of their amount per area unit, an essential intensification of ramification, formation of powerful anastomoses, numerous dense plexuses and arcades. Fasciculi of neurites covered with glia are formed. The cerebral neurite-stimulating protein is, evidently, one of neuron-growing factors, regulating development of the nervous system in the organism.

Animals↗