The consumption of the antihypertensive means in Russia and abroad is analyzed, the efficiency and the cost of the recommended circuits of treatment of hypertonic heart disease are investigated and appreciated, taking into account the international researches. The list of medicines, offered as the standard of pharmacotherapy and for supply of military-medical establishments in peace time is made. The offered spectrum of preparations made by a domestic industry permits to ensure effective and inexpensive treatment of blood hypertension according to circuits existing for a today's day.

Drug Therapy↗

PubMed1990

[Effect of the producer type on the nature of antibiotic fermentation].

Fermentation processes in production of bacitracin, a polypeptide antibiotic by Bacillus licheniformis, and oleandomycin, a macrolide antibiotic by Streptomyces antibioticus, were studied comparatively. It was shown that the antibiotic-producing actinomycete was characterized by a prolonged phase of growth retardation. The highest efficiency of the control actions was observed at the beginning of the fermentation. They were aimed at intensifying the substrate usage during the growth phase and activation of cell metabolism. Controlled cultivation of the Bacillus representative was based on its capacity of achieving the maximum growth rate possible under the certain conditions. Therefore, an increase in the quantity of the synthesized antibiotic was due, under such conditions, to inhibition of the culture growth by various means including lower mass exchange intensity.

Bacillus↗

PubMed1987

[Characteristics of the oleandomycin producer inoculum at various levels of antibiotic biosynthesis].

Parameters of the oleandomycin-producing organism metabolism were studied at the stage of inoculum when intensity of the antibiotic biosynthesis was increased by treating the spores with a surface active substance (twin-21). It was shown that the inoculum producing later at the stage of fermentation higher quantities of the antibiotic was characterized by certain peculiarities. In particular, there were observed a shorter lag phase, a higher specific growth rate and a higher rate of accumulating the medium components at lower pyruvate levels in the exponential phase, higher activity of succinate dehydrogenase and higher levels of ATP in the mycelium during this period.

Fermentation↗

PubMed1987

[Effect of surface-active substances on carbohydrate metabolism in the producer of oleandomycin].

Activity of glucose-6-phosphate dehydrogenase (I), fructose diphosphate aldolase (II) and succinate dehydrogenase (III) and content of pyruvate (IV) in the mycelium of the oleandomycin-producing organism were studied during increased intensity of the antibiotic synthesis. The increase in the intensity of the antibiotic synthesis was induced by exposure of the spores to a surface active substance, twin-21. After the exposure a rise in the activity of all the three enzymes in the phase of the culture intensive growth was observed. During the antibiotic intensive production the cultures with increased antibiotic production levels were characterized by significantly higher activity of I as compared to the control while the activity levels of II and III were approximately the same. It was shown that concentration of IV in the mycelium during the antibiotic intensive biosynthesis decreased and the decrease was more pronounced after exposure to twin-21.

Carbohydrate Metabolism↗

PubMedDate not supplied

[Effect of surface-active agents (tween-21) on indices of energy metabolism in oleandomycin producers].

The specific growth rate of Streptomyces antibioticus, a producer of oleandomycin, and the specific rate of the antibiotic accumulation in the culture medium during fermentation were investigated. On the basis of the results obtained the fermentation period was divided into 7 phases of development. The culture treated with the surfactant (Tween-21) is characterized by a higher specific growth rate during the whole fermentation and a higher specific rate of the antibiotic accumulation at the stage of the highest production as compared to the control. The ATP content, the value of the adenylate energy charge and the contents of high-molecular weight polyphosphates in the mycelium were examined. In the phase of the intensive growth St. antibioticus was characterized by a higher ATP level and a higher energy charge. More active accumulation of polyphosphates was observed in the late intensive growth phase. It was also found that after the treatment of the culture with Tween-21 it utilized polyphosphates more actively during the antibiotic biosynthesis.

Culture Media↗

PubMedDate not supplied

[Analysis of glucocorticoids in biological objects using spectrofluorometry].

A method for analysis of corticosterone and hydrocortisone in one and the same sample is proposed. It is based on derivative spectrofluorometry. The sensitivity of the method is 0.04 mg/l for hydrocortisone and 0.01 mg/l for corticosterone.

Adrenal Glands↗