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Biomedical subjects

A V Shcherbakov

Publications and source records attributed to A V Shcherbakov.

10 recordsLinked to original sources
PubMed1998

[Primary structure of gene H of cattle plague virus strain K].

Synthesis, cDNA cloning, and identification of H gene nucleotide sequence of rinderpest virus (RPV) K strain are carried out. Analysis of the identified nucleotide sequence has revealed the single open reading frame encoding a protein consisting of 609 amino acids with molecular weight of 68 kDa. The mean nucleotide homology between H genes of K, Kabete O and L strains in 88.0%, the mean amino acid homology of the corresponding proteins is 88.2%. RPV K strain hemagglutinin contains 5 potential glycosylation sites. The position of all 13 cystein bases is identical to positions in H proteins of RPV Kabete O and L strains. Studies of the hydrophobic profile of the compared proteins have shown 2 potential transmembrane fragments.

Animals↗
PubMed1998

[Molecular basis of changes in biological properties of foot and mouth disease virus of subtype A22].

Primary structure of capsid proteins and RNA polymerase of three closely related strains of foot and mouth disease virus (FMDV), subtype A22, differing by biological properties (the initial epitheliotropic strain A22 550 and its derivatives: thermoresistant myotropic A22 550/4 and thermosensitive attenuated A22 645) are compared by nucleic acid sequencing and analysis of the amino acid sequencing. The study revealed 1 substitute in VPI and 8 in RNA polymerase in the myotropic variant and 1 substitute in VP2, 2 in VP3, 13 in VP1, and 3 in RNA polymerase. Alteration of A22 550/4 tropism is probably due to a single substitution Gly 145-->Thr in the RGD site of capsid protein VP1. Analysis of the origin and biological properties of the attenuated strain A22 645 and the results of studies of the primary structure of proteins permit us to hypothesize that attenuation is polygenic, caused by adaptation to a heterologous host (continuous porcine cell culture), and can be expressed by changes in the structure of virus antireceptor providing its binding to cell receptors. Sites responsible for the reproduction of A22 FMDV at certain temperatures are presumably located in RNA polymerase.

Amino Acid Sequence↗
PubMed1997

[A simple method for RNA isolation and purification].

RNAs from Escherichia coli cells, Syrian hamster kidney cells, foot-and-mouth disease virus, and Newcastle disease virus were isolated using glass fiber filters GF/F or GF/C. The RNA was reversibly adsorbed on the filters in the presence of 2 M guanidine thiocyanate and 50% ethanol (or isopropanol) and eluted with water. The fraction composition of the isolated RNA depended on the guanidine thiocyanate and alcohol concentrations in the adsorption and washing procedures. The RNA preparations obtained by this method can be used in reverse transcription and reverse transcription-polymerase chain reaction without additional purification.

Animals↗
PubMed1996

[Use of aerosol A-300 amd GF/F (GF/C) filters for purifying fragments of DNA, plasmid DNA, and RNA].

Aerosil A-300 and GF/F (GF/C) filters were used for purifying plasmid DNA and intact RNA as well as for the recovery of DNA fractionated on agarose gels. In the presence of guanidinium thiocyanate Aerosil A-300 and GF/F (GF/C) filters selectively bind nucleic acids, while proteins, agarose polysaccharides and salts are washed off. Nucleic acids desorbed from the Aerosil and filters are available immediately as substrates: DNA for restriction analysis, ligation and sequencing; RNA--for RT-PCR.

DNA↗
PubMed1995

[The structural reorganization of the wall of the inferior mesenteric vein and of its vascular-neural components in fetuses and children].

Structural changes of the inferior mesenteric vein wall and it vascular-nervous apparatus were studied in 93 inferior mesenteric veins of human foetuses and children, aged to 3 years using the complex of histological and neuromorphological methods. While changing the structure of the wall became more complicated in myocyte and fibres number.

Child, Preschool↗
PubMed1988

[The human umbilical cord vein in the surgical treatment of acute arterial thrombosis].

The authors' experience with the application of bioprostheses of the human umbilical cord vein allows them to speak of its effectiveness and positive properties such as: 1) thromboresistance, 2) areactivity, 3) immunological compatibility, 4) firmness, 5) possibility of prolonged storage, which make it possible to be used in urgent surgery of the vessels. Positive results were obtained in 89.47% of the cases and the authors think that using bioprostheses of the human umbilical cord vein is expedient in the surgery of acute arterial thromboses.

Acute Disease↗
PubMedDate not supplied

[Recombinant non-structural 3A, 3B and 3AB proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle].

Recombinant proteins 3A, 3B and 3AB were obtained by expression in Escherichia coli and purified by metal-chelate chromatography. The proteins were used as antigens in indirect ELISA to differentiate vaccinated and infected cattle. While testing 200 sera from cattle 3A-ELISA was more sensitive and specific than 3B- and 3AB-ELISA. Compared with "Chekit FMD-3ABC", 3A-ELISA showed the same level of specificity and higher level of sensitivity.

Animals↗
PubMedDate not supplied

[Cloning fragments of the RNA polymerase gene of an attenuated variant of the foot-and-mouth disease virus A22].

The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.

Aphthovirus↗