PubMed Health⌕ Search

Biomedical subjects

A V Vlasov

Publications and source records attributed to A V Vlasov.

At least 19 recordsLinked to original sources
PubMed2000

Spectroscopic study of low temperature interactions in Sm-mesogenic cyanophenyl co-condensates.

The IR- and UV-Vis spectroscopic study of samarium-mesogenic 4-pentyl-4'-cyanobiphenyl low temperatures co-condensates was made in temperature range 90-300 K. Two labile complexes with different metal/ligand ratio of 1:2 and 1:1 were found. They were characterized by methods of IR- and UV-spectroscopy. The solid state transformation of complex Sm(5CB)2 to Sm(5CB) was shown to take place at 170-210 K. The kinetics of the process revealed a wide distribution of complex reactivity and of reaction activation energy.

Biphenyl Compounds↗
PubMed2000

[Chemical ribonucleases. 2. Design and hydrolytic properties RNase mimetics based on diazabicyclo[2.2.2]octane with various positive charges].

A procedure was proposed allowing one to synthesize RNA mimics on the basis of conjugates of diazabicyclo[2.2.2]octane with imidazole bearing a varying number of positive charges (nDm series, where n is the number of positive charges at neutral pH, m is the code of an imidazole-containing fragment of the catalytic domain: 1, histamine; 2, histidine methyl ester). The hydrolytic activity of six compounds of this series was studied under physiological conditions using in vitro transcript of human mitochondrial tRNA(Lys) as a substrate. It was shown that the rate of RNA hydrolysis with nDm conjugates rises with an increase in the number of positive charges: an approximately 30-fold acceleration of hydrolysis was observed with an increase in the total charge of the construct from +2 to +4.

Bridged Bicyclo Compounds, Heterocyclic↗
PubMed1999

[Synthetic ribonucleases. 1. Synthesis and properties of conjugates, containing an RNA-binding fragment based on lysine residues and an RNA hydrolyzing fragment, bearing an imidazole residue].

A series of RNA-hydrolyzing constructions was synthesized on the basis of peptide-like molecules containing residues of L-lysine, histamine and histidine methyl ester. These were shown to hydrolyze RNA effectively at neutral pH values. The in vitro transcript of tRNA(Lys) from human mitochondria and a tRNA-like fragment of RNA of Turnip Yellow Mosaic Virus were used in the experiments. Our chemical RNases quantitatively depolymerize some definite sequences (CA > or = UA > CG >> UC, CC, or CU) in both RNA molecules under optimum conditions. Moreover, no other sites were affected and no statistical hydrolysis was observed even after prolonged RNA incubation with the compounds of this series. The depolymerization rate of the RNA substrates exhibits a complex dependence on the concentration of ions of monovalent metals and on the concentration of the artificial ribonucleases.

Humans↗
PubMed1996

[Immediate and long-term results of the treatment in cancer of the large intestine].

Operative treatment of 200 patients with carcinoma of the rectum is analysed. Planned operations were conducted on 101 patients, postoperative mortality was 7.8%. Surgery was undertaken in 93 patients with acute intestinal obstruction, postoperative mortality was 15.05%. Changes of the policy of treatment of patients with acute intestinal obstruction reduced postoperative mortality. Five-year survival was 67.1%.

Colectomy↗
PubMed1995

Development information systems for clinical laboratories and their integration into HIS.

The basic laboratory program module was developed using a summarized information model of a clinical laboratory's functionality. Then, information systems for laboratories of different specialties were quickly implemented by adaptation, addition, and tuning of this basic module. Integrating this system into an HIS considerably improved the interaction between clinical departments and laboratories.

Clinical Laboratory Information Systems↗
PubMed1991

[Automation of hematologic studies in a clinical-diagnostic laboratory in a multi-profile hospital].

The authors share their experience gained with automation of hematologic investigations at a clinical diagnosis laboratory. A CDL information system, basing on personal computers, has been created, functioning in complex with hematologic autoanalyzers. The reports on the laboratory's activity are made automatically, as is the laboratory register and processing of applications for tests.

Clinical Laboratory Information Systems↗
PubMed1990

[A comparative study of immediate and long-term results of the treatment in cancer of the distal part of the stomach].

Treatment results were studied in 117 cases of cancer of the distal part of the stomach; the patients had undergone subtotal resection. Postoperative lethality after Hofmeister-Finsterer's resection was 15.1%, after valve anastomosis-5.9% (overall lethality-8.3%). Postoperatively, patients with valve anastomosis did better and suffered surgery-related functional disorders less frequently than those treated after Hofmeister-Finsterer. 104 (97.2%) patients were followed up. Five-year survival was 45.2%; ten-year-32.2%.

Aged↗
PubMed1990

[The effect of the peptide fractions of neutrophilokines on the immune response of mice infected with Staphylococcus aureus].

The influence of immunostimulating and immunosuppressing neutrophilokine fractions on the immune response of mice with staphylococcal infection has been studied. When injected into mice with staphylococcal infection, the immunostimulating fraction of the supernatants of activated neutrophils has been found to restore the activity of the immune system of the infected animals. The secretory products of intact neutrophils do not change or decrease the capacity of the animals for immune response under the conditions of the infectious process.

Animals↗
PubMed1989

A direct selection procedure for isolating yeast mutants with an impaired segregation of artificial minichromosomes.

The nondisjunction of artificial yeast minichromosomes (2:0 segregation events) during mitosis is accompanied by the appearance of cells containing more than one copy of the minichromosome. A mathematical simulation of this process has demonstrated that under certain conditions, a nondisjunction of the minichromosomes may result in their accumulation in a considerable portion of the cell population. An increase in the copy number of artificial minichromosomes as a result of impaired segregation has been used to develop a new experimental procedure for directly selecting yeast mutants showing an impaired segregation of artificial minichromosomes during mitosis. Four new genes, AMC1, AMC2, AMC3, and AMC4, which control the segregation of artificial minichromosomes in mitosis, have been identified (AMC3 and AMC4 are mapped to chromosome IV and VII, respectively). Mutations in the genes AMC1-AMC4 also affect the mitotic transmission of natural chromosomes. We suggest that the genes AMC1, AMC2, AMC3, and AMC4 control the segregation of natural chromosomes in yeast.

Chromosomes↗
PubMed1989

[Study of latex-induced luminol-dependent chemiluminescence of neutrophils].

The effects of various polystyrene monodispersed latexes on neutrophil chemiluminescence (CL) induction have been studied. The studies have revealed that latexes may be employed as neutrophil CL activators. Latexes with a diameter of 1.7 micron could induce CL of the highest intensity. An increase or decrease in the diameter of latex particles reduced the intensity of neutrophil CL induction. Gamma-globulin or immunoglobulin coating of latex lowered, whereas zinc methacrylate coating enhanced the intensity of the reaction. Since latexes induce a maximum respiratory outburst in the first two minutes after their introduction in cellular suspension, they may be referred to as rapid inductors of neutrophil CL.

Humans↗
PubMed1989

[Changes in the immunotropic activity of neutrophilokins in the course of experimental staphylococcal infection].

The influence of the products secreted by activated neutrophils (neutrophilokins) of mice, both intact and infected with staphylococci, on the activity of mouse spleen cells in the graft-versus-host reaction, immune response to sheep red blood cells and the antigen-presenting function of peritoneal macrophages was studied. Neutrophilokins of intact mice stimulated the activity of immunocompetent cells. Neutrophilokins obtained from infected mice on day 3 after infection produced an immunosuppressing effect. On day 7 after infection the immunostimulating activity of neutrophils was restored and showed practically no difference from the normal level.

Animals↗
PubMed1989

[Comparative evaluation of late results of the Hoffmeister-Finsterer gastrectomy and the method of valve anastomoses in peptic ulcer].

Comparative assessment of the remote results of Hoffmeister-Finsterer gastrectomy with creation of valve anastomoses has demonstrated rare occurrence of postoperative complications owing to anastomoses. The operation is simple as concerns operative techniques and can be recommended as operation of choice in the treatment of peptic ulcer.

Anastomosis, Surgical↗