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Biomedical subjects

A Valentino

Publications and source records attributed to A Valentino.

At least 19 recordsLinked to original sources
PubMed1992

Determination of organophosphorus pesticides in fruits by on-line size-exclusion chromatography-liquid chromatography-gas chromatography-flame photometric detection.

The determination of organophosphorus pesticides in fruits by size-exclusion chromatography (SEC) on a polystyrene column coupled on-line to a gas chromatography (GC) system was unsatisfactory as a result of interfering peaks in GC. A liquid chromatography step on silica gel was therefore inserted between the SEC and GC steps to filter out polar by-products. Samples of fruit (apples, grapes and kiwi fruits) were extracted, then the extract filtered or centrifuged and injected into an automated on-line SEC-liquid chromatography-GC-flame photometric detection. Recoveries were about 95% and the detection limits about 1 ng/g.

Chromatography, Gas↗
PubMed1984

[Therapy with bromocriptine and behavior of various hormones in psoriasis patients].

The activity of bromocriptin has been clinically tested on 18 patients suffering from psoriasis. The plasma levels of Human Growth Hormone (HGH), Human Adrenocorticotropic Hormone (ACTH), Thyroid Stimulating Hormone (TSH), Prolactin (PL), Aldosterone and Cortisol were investigated in these 18 patients along with 35 untreated psoriatic patients and 19 normal subjects. Bromocriptin was shown to be effective in 13 of our 18 psoriatic patients. Plasma levels of HGH, ACTH, and Aldosterone, measured in all 53 psoriatic patients, were found to be higher than normal in 11, 26 and 40 patients respectively (HGH and Aldosterone: p less than 0,005; ACTH: p less than 0,001).

Adrenocorticotropic Hormone↗
PubMed1984

[Cosmetic acne and a test of comedogenicity].

A comedogenic test was carried out on the internal ear canal of four adult, masculine, albino rabbits, using butter of cacao and linseed oil, both known to be present in various cosmetic products. Histologic observation after 14 days showed follicular hyperkeratosis conferming the validity of this test.

Acne Vulgaris↗
PubMed1984

[Differences in the culture of pigment cells from various sources].

In order to look for a new culture system suitable for investigating on melanocyte biology, in-vitro experiments have been carried out on pigmented basal cell carcinoma, pigmented seborrhoeic keratosis and melanocytic nevus. Melanocytes cultured from pigmented basal cell carcinoma and pigmented seborrheic keratosis are dendritic and DOPA positive. These cells can be considered similar to those of normal skin. Pigmented cells cultured from nevi are quite different. They are spindle-shaped and only to a little extent are DOPA positive. These findings suggest that nevus cells could be non mature melanocytes stopped at an intermediate stage of differentiation.

Basal Cell Carcinoma↗
PubMed1984

[Use of monoclonal antibody 225.28S in the study of nevus cells in culture].

The aim of this work is to identify and purify cultured nevus cells. We used for our study monoclonal antibody 225.28S. This antibody reacts with a surface antigen which is expressed by nevus cells and melanoma cells, but it does not react with normal melanocytes. We studied 10 dermic nevi and we observed that antigenic determinant survives in cultured nevus cells. These results allowed us to employ the method of panning for purify cultured nevus cells.

Antibodies, Monoclonal↗
PubMed1984

[Endothelial cell culture as a model for the study of wound healing].

Endothelial cells culture can be considered a reliable method for investigating about granulation tissue production in wound healing and for evaluating the pharmacological action of some chemicals on granulation tissue development. Endothelial cells have been obtained from human umbilical cords after trypsin treatment and their endothelial origin has been demonstrated by light microscopy, by immunofluorescence against factor VIII associated protein and by the platelet adhesion assay. The influence of fibronectin as substratum and of hyaluronic acid as soluble factor on adhesion and growth of endothelial cells has been investigated. Both these substances, but especially hyaluronate, determine a better attachment and an increase in the growth rate when compared with control cultures plated on plastic substratum and without hyaluronic acid in the culture medium.

Cell Adhesion↗
PubMed1978

DNA-repair after UV-irradiation in skin fibroblasts from patients with actinic keratosis.

Autoradiographic counting technique was utilized to measure the ultraviolet-induced unscheduled DNA synthesis of skin fibroblasts from 12 patients with chronic actinic keratosis and from 12 healthy donors of about the same age. In order to reveal a possible regional difference of DNA repair between the parts of the body ordinarily exposed and those parts unexposed to sunlight, two cell strains were used for each examined subject; one developed from the forehead skin and the other from the abdominal or axillary skin. Unscheduled DNA synthesis appeared depressed in actinic keratosis patients, as compared with controls. In all examined subjects however cell strains from exposed skin showed a DNA repair more active than cell strains from unexposed skin. These findings show that skin cancer may be promoted in actinic keratosis patients by a defect of DNA repair. The exalted DNA repair of chronically sun exposed skin is probably the consequence of a defensive process caused by enzymatic induction.

Aged↗
PubMed1977

[Reconstructive surgery of the biliary tract].

Reconstructive surgery of the bile ways involves a great commitment owing to the technical difficulties, the diversity of the nature, site and extent of the alteration to be treated, and because of the particular anatomical structure of the formations which have to be operated. The conditions that can benefit most from this type of surgery are represented by preoperative lesions of the bile ways and particularly stenoses which but not only follow such lesions if they are not treated in time, but may also follow other processes of a benign nature such as calculosis, cholangitis, anomalies of the bile ways treated wrongly during previous cholecystectomy, and at times malignant processes. Reconstructive surgery in bile way surgery means direct re-establishment of the duct to enable bile to flow from the liver to the duodenum. It presupposes a number of local conditions, such as the circumscription of the condition so that the stenotic segment can be removed and long-lasting end-to-end anastomosis effected. When it is possible, this must be considered the ideal procedure since it offers anatomophysiological recovery of bile way function. Operations have also been performed using various vein, artery, appendix, fascia lata, etc. grafts and prostheses using foreign matter such as rubber, vitallium,dacron, etc. tubes but results have always been hazardous and debatable. Personal cases and techniques are reported.

Adult↗