Biomedical subjects

A Vanheusden

Publications and source records attributed to A Vanheusden.

3 recordsLinked to original sources

PubMed2001

[Impression technics in implantology].

The implant-borne prostheses have become an integral part of the oral rehabilitation procedures. The aim of this article is to describe the most current impression techniques for oral implant prosthetics used at the University of Liège, Belgium. Impression protocols adapted to various prosthetic procedures are described step-by-step through several clinical cases. Emphasis is put on the means necessary for achieving a precise adaptation and a passive fit of the final prosthetic suprastructure.

Cementation↗

PubMed1999

The influence of cortical perforations and of space filling with peripheral blood on the kinetics of guided bone generation. A comparative histometric study in the rat.

The aim of the present study was to evaluate the influence of cortical perforations and of peripheral blood addition in guided bone generation beyond the skeletal envelope in rats. A total of 30 isogenic adult rats were divided into 3 equal groups. In each rat, two hollow parallelipipedic titanium chambers were placed bilaterally on the calvaria after a periosteal skin flap was raised. While on the right sides (controls) the osseous surface was left intact and the chambers were empty, the cortical bone under the left-side chambers (test sites) was perforated with nine 0.8 mm-diameter holes (group I), or left intact but with the chambers filled with a clot of peripheral blood (group II). In group III, both procedures were combined in the test sites. The healing was assessed at 4, 8 and 16 weeks after surgery by histologic and computer-assisted histometric analysis. The results demonstrated a substantial augmentation of on average 141% (SD 18) of the skull's thickness after 16 weeks in the controls, indicating that a predictable bone formation can be achieved beneath completely occlusive barriers over a non-injured cortical layer. In all test groups, a significantly larger bone augmentation was observed after 16 weeks compared to the control sites 172.8% (SD 41.7) in group I (P < 0.05), 172.0% (SD 18.4) in group II (P < 0.05) and 221.5% (SD 42.3) in group III (P < 0.001), demonstrating that stimulating blood supply and bone forming cells access by cortical perforations and/or blood clot addition enhances de novo bone formation in this experimental model.

Animals↗

PubMed1998

In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCL.

Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium.

Adolescent↗