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Biomedical subjects

A Ville

Publications and source records attributed to A Ville.

10 recordsLinked to original sources
PubMed1999

Heart block following propofol in a child.

We present the case of a nine-year-old boy afflicted with Ondine's curse, who developed complete atrioventricular heart block after a single bolus of propofol for induction of anaesthesia for strabismus surgery. Ondine's curse, the other name for congenital central hypoventilation syndrome, is characterized by a generalized disorder of autonomic function. Propofol has no effect on the normal atrioventricular conduction system in humans but it reduces sympathetic activity and can highly potentiate other vagal stimulation factors. Heart block has been documented after propofol bolus use in adults but, to our knowledge, not in children. It would appear that propofol is not a good choice for anaesthesia in congenital central hypoventilation syndrome.

Anesthetics, Inhalation↗
PubMed1990

Enzyme coupling method on calibrated nylon spheres: application to the selective trypsinization of histones in chromatin.

A new method consisting of a two-step activation was developed in order to covalently immobilize enzymes on calibrate nylon 66 spheres. This efficient method associates for the first time peptide bond cleavage and O-alkylation of the support. Optimal conditions for activation and protein coupling were defined, and immobilized trypsin was used to investigate the histone accessibility on chromatin. This approach, which allows us to degrade first progressively H1, indicates that H4 seems inaccessible both in relaxed and condensed chromatin.

Chemical Phenomena↗
PubMed1984

[Fixation of antibodies by covalent bonds to polystyrene: use of the immunoenzyme technic for the detection of Escherichia coli K99 in calf feces].

A solid phase enzyme immunoassay using antibodies covalently bound to polystyrene balls is developed. Results show the same sensitivity as the method with antibodies adsorbed to solid support. By means of K99+ E. coli detection on diarrhoeic calves, results are compared with bacteriological findings. The interest of the method is discussed.

Animals↗