[Characterization using phage typing of methicillin-resistant Staphylococcus aureus strains causing outbreaks].
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Bacteriophage Typing↗
Biomedical subjects
A Vindel
Publications and source records attributed to A Vindel.
Staphylococcus aureus resistant to methicillin.
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Humans↗
Development of a new set of phages as an epidemiological marker in Staphylococcus epidermidis causing nosocomial infections.
We describe the isolation of a new set of phages for typing Staphylococcus epidermidis. One hundred and eighty-two phages were obtained from S. epidermidis strains of human origin. Twelve phages were selected according to their potency and their lytic activity studied. Twenty phages of the Dean and Williams' set were also studied. Phage-typing was undertaken at 100 x RTD, 1000 x RTD and after heat treatment at 48 degrees C. When the two sets of phages were compared separately similar figures were obtained. When the two typing sets were combined, the percentage of typability for the 182 bacterial strains increased to 29.1% using 1000 x RTD and to 75.3% after heat treatment.
Bacteriophage Typing↗
Extracellular enzymes of fecal strains of Pseudomonas aeruginosa.
During a three year study 67 strains of Pseudomonas aeruginosa were isolated from stools of 67 patients with gastroenteritis. Serotypes 3 and 6 were the most frequent. The three strains that were serotype 11 were also the only beta-galactosidase producers. All strains were strongly pigmented. Over 90% of the isolates produced hemolysin, gelatinase, protease (caseinase), fibrinolysin, lecithinase and elastase. Fecal carriers of Pseudomonas aeruginosa can be considered a source of dissemination of potentially virulent and invasive strains.
Adolescent↗
Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection.
A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 X RTD, phage-typing after heat-treatment (48 degrees C), thermal shock (56 degrees C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reduced by 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful. A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.
Bacteriophage Typing↗
Anterior pituitary of the macaca mulatta monkey: an electron microscopic view.
An electron microscopic study was performed of the cells which constitute thar anterior pituitary of the Macaca Mulatta female monkey. The characteristics of each cell are described, and the findings are illustrated schematically. Different forms of hormonal secretion are observed and described.
Animals↗
Persistence and variability of Stenotrophomonas maltophilia in cystic fibrosis patients, Madrid, 1991-1998.
During 1991 to 1998 at least one Stenotrophomonas maltophilia pulmonary infection was observed in 25 (24%) of 104 cystic fibrosis patients at the same unit of our hospital in Spain. Ribotyping and pulse-field gel electrophoresis (PFGE) characterization of 76 S. maltophilia isolates from these patients indicated an overall clonal incidence of 47.1%, reflecting new strains in 44% of patients with repeated positive cultures for S. maltophilia. Six patients with repeated episodes were persistently colonized (> or = 6 months) with the same strain. S. maltophilia bacterial counts were higher (geometric mean, 2.9 x 10(8) cfu/mL) in patients with repeated episodes than in those with a single episode (8.4 x 10(4) cfu/mL, p < 0.01). Single episodes of S. maltophilia occurred in patients < 10 years of age (43% [6/14]), whereas chronic colonization occurred more frequently in older patients (> 16 years of age).
Adolescent↗