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A W Janes

Publications and source records attributed to A W Janes.

4 recordsLinked to original sources
PubMed1977

Serial infusion effects of hydroxyethyl starch on ESR, blood typing and crossmatching and serum amylase levels.

Eight normal healthy volunteers underwent a series of three plasmaphereses, prior to the infusion of 250, 500, and 750 ml hydroxyethyl starch (HES), respectively, in order to ascertain the effect of this agent on erythrocyte sedimentation rate (ESR), blood typing and crossmatching, and serum amylase levels. The bolus injection of either 500 or 750 ml HES produced a significant increase in the ESR, which was sustained over a 5-hour period. Rouleaux formation was observed to be dose related and only observed following administration of greater than 500 ml (575 mg/dl whole blood concentration). The rouleaux formation was, however, easily dispersed by the addition of saline. Blood typing and crossmatching studies were normal, but caution must be taken in regard to false positives when the estimated blood concentration of HES exceeds 575 mg/dl. a-Amylase activity corrected for hemodilution was not significantly altered immediately following infusion of HES in these reported amounts. Recommendation of a new method of HES administration during centrifugal leucapheresis is discussed.

Amylases↗
PubMed1976

Utilization of a new strength citrate anticoagulant during centrifugal plateletpheresis. I. Assessment of donor effects.

A reduction of donor effects during centrifugal plateletpheresis with the Haemonetics Blood Processor was achieved by reducing the concentration of the citrate anticoagulant. Serum citrate and ionized calcium levels, immediately and 1 h post-pheresis, were affected to a lesser extent by using 5.0 g total ionized citrate (TIC) THAN WITH EITHER 8.0 G OR 11.0 G. Total calcium, bicarbonate, prothrombin time, partial thromboplastin time, ECG, and platelet counts were affected to a similar degree by all three TIC formulations. The total number of platelets collected per litre of blood processed was not significantly different among the three TIC formulations. In vitro studies employing the screen filtration pressure (SFP) technique showed no evidence of platelet aggregates in whole blood collected into either 0.01 M or 0.005 M citrate and agitated or left stationary at room temperature for 5 h. The use of different citrate concentrations in plateletpheresis is discussed.

Anticoagulants↗