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Biomedical subjects

A W Wayne

Publications and source records attributed to A W Wayne.

8 recordsLinked to original sources
PubMed1985

Olfactory, gas chromatographic and mass-spectral analyses of fecal volatiles traced to ingested licorice and apple.

Volatile components of two foodstuffs with characteristic aromas, apple and licorice, and fecal samples obtained from subjects on high-apple and licorice diets, were analyzed by head-space gas chromatography and gas chromatography-mass spectrometer. The volatile compounds characteristic of the odors of apple and licorice were detected by "sniffing" the GC effluent. The aromatic component of licorice was identified by GC-MS as anethole [1-methoxy-4-(2-propenyl) benzene]. The aromatic component of apple could not be chemically characterized by our GC-MS system even though readily detected by the nose. Head-space, GC-MS analysis of fecal volatiles offers a means of tracing odorous and non-odorous components to ingested foodstuffs with potential applications to clinical and forensic medicine and anthropology.

Allylbenzene Derivatives↗
PubMed1984

Candidate products of avian myeloblastosis virus oncogene region produced by in vitro translation of genomic RNA.

Avian myeloblastosis virus (AMV) genomic RNA was translated in vitro to identify the AMV transforming gene (myb) product(s). Full-length RNA yielded, in addition to the expected Pr76gag, a gag-related product of 92,000 daltons which did not appear to be a gag-oncogene fusion protein. Subgenomic AMV RNA in vitro translation, nonstructural products (specific for AMV) were of 54,000, 49,000 and 34,000 daltons. Based on their sizes and similar peptide maps for the smaller two proteins, it is proposed that they derive from the myb region of AMV.

Avian Leukosis Virus↗
PubMed1982

A photometric study of the standard Philadelphia (Ph1) translocation of chronic myeloid leukemia (CML).

A newly developed form of high resolution microscope photometry has been employed in a study of the relationships between the chromosomes involved in the standard Philadelphia (Ph1) translocation of chronic myeloid leukemia (CML). The intention of the investigation was to provide quantitative data on the relative amount of material exchanged between the No. 9 and No. 22 chromosomes. A marked consistency in the amount of material lost from the Ph1 was observed, and the balanced nature of the standard Ph1 translocation was confirmed. The data are also in accordance with the concept of a reciprocal exchange between the No. 9 and No. 22 chromosomes.

Bone Marrow Cells↗
PubMed1981

The use of high resolution microscope photometry in the discrimination of chromosome bands.

High resolution microscope photometry was used to discriminate between regions of differing density along the length of a banded metaphase chromosome. It has been demonstrated that photometry can be performed on such material to give a higher level of effective resolution than has hitherto been obtained. Using conventional visible light microscope photometry it is possible to obtain useful comparative densitometric information from measurements performed at a point separation equivalent to 0.05 micrometer in the object plane. The practical details of the equipment employed and the theoretical basis of the study are discussed.

Chromosome Banding↗
PubMed1979

The significance of Ph1 mosaicism: a report of six cases of chronic granulocytic leukaemia and two cases of acute myeloid leukaemia.

Six cases of chronic granulocytic leukaemia (CGL) and two cases of acute myeloid leukaemia (AML) with dual populations of karyotypically normal and Philadelphia (Ph1) chromosome-positive cells are described. GTG and QF-banding characterized the Ph1 as resulting from a 9/22 translocation in all eight cases. Four of the patients suffering from CGL presented with 100% Ph1-positive bone marrows, and after receiving intensive chemotherapy, karyotypically normal cells were demonstrated. The other two patients with CGL showed Ph1 mosaicism at presentation. The two patients with AML exhibited Ph1 mosaicism at presentation and throughout the course of the disease. In both of these patients, a marker No. 10 chromosome was found in some of the Ph1-positive cells and in one hyperdiploidy was observed to have developed only in the clone with the additional chromosome anomaly.

Adult↗
PubMed1977

Sudden toxicity of methadone in monkeys: behavioral and electrophysiological evidence.

A sudden and potentially lethal toxic reaction to a previously well-tolerated maintenance dose of methadone occurred in 4 of 6 monkeys. The reaction was characterized by gross behavioral and respiratory depression and a marked attenuation of both early and late components of the visual evoked response with an increase in most latencies. The nature of the evoked response alteration suggests a widespread central nervous system depressant effect of the drug during toxicity. Concomitant with the toxic reactions were dramatic increases in plasma methadone concentrations. Therefore the observed changes in sensitivity to methadone would appear to be the consequence of a sudden shift in pharmacokinetics resulting in toxic plasma concentrations.

Animals↗