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A Wijsman-Grootendorst

Publications and source records attributed to A Wijsman-Grootendorst.

7 recordsLinked to original sources

[The attitude of Dutch women of Turkish ancestry towards measures for the prevention and treatment of vitamin D deficiency; the results of focus group interviews].

OBJECTIVE: To obtain insight into the attitude of Dutch women of Turkish ancestry towards various possible measures for the prevention and treatment of vitamin D deficiency. DESIGN: Qualitative, focus group interviews. METHOD: Four focus group meetings of 4-13 women of Turkish ancestry (mean age: 31.8-47.1 years) were held in community centres in Amsterdam. Discussions were held with the aid of an interpreter in response to questions about exposure to sunlight, vitamin D supplements, fortification of foods with vitamin D, and the consumption of vitamin D rich foods. The discussions were recorded, translated, and transcribed, and the recurring themes were analysed. RESULTS: Participants had a strong preference for vitamins from 'natural sources' as compared with vitamin supplements. Concerns about weight gain and overdose as a result of vitamin supplement use were expressed frequently. Most women only found the use of vitamin D supplements acceptable if prescribed by a physician. For religious/cultural reasons, many women were not willing to alter their dress to expose more skin to sunlight, or were only willing to do so in the strict absence of men that are not members of the family. Participants were reluctant to consume vitamin D-fortified margarine products because of concern about the possible presence of pork fat and the fear that consumption of these products would induce weight gain. CONCLUSION: Among Dutch women of Turkish ancestry, cultural and religious beliefs and concern about the possible side effects can impede the acceptance of advice on how to combat vitamin D deficiency. This should be taken into consideration when giving information on the prevention and treatment of vitamin D deficiency.

Adult↗

Upgrading unsatisfactory cervical smears with the MiB-1 method.

When an unsatisfactory smear (thick inflammatory infiltrate or blood influencing the staining characteristics of the epithelial cells) is restained for MiB-1, the diagnostic proliferating cells are visualized, and the MiB-1-positive smears can be thus upgraded as borderline, grade I, II, and III, corresponding with the cytologic diagnoses of, respectively, ASCUS, CIN I, II, and > or = III. In a period of 18 months, 2,068 unsatisfactory smears out of a material of 84,817 smears were restained for MiB-1. In the unsatisfactory group, significantly more abnormal smears were detected than in the satisfactory group. Seventy-five of the unsatisfactory group were biopsied because of the MiB-1 findings: Three women proved to have severe dysplasia, four had carcinoma in situ, and three had invasive carcinoma. The per mileage for invasive cervical carcinoma was ten times larger in the unsatisfactory group than in the satisfactory group; thus the MiB-1 method has further enhanced our diagnostic acumen in this difficult type of smears.

Antigens, Nuclear↗

Involving the general practitioner in screening for cervical cancer.

In the Leiden region, the cervical cancer screening programme for the age group 35-54 years shifted in 1989 from one in which the smears were taken by specially trained paramedical personnel in health centres to a programme where the general practitioner is the smear taker. Hence, it is possible to compare the results of the two types of screening to evaluate whether involvement of general practitioners leads to better uptake of screening. In the 6 year period evaluated in this paper, the 3-yearly cytological rate per thousand for severe dysplasia or worse increased from 1.00 to 3.40; when stratified by age the positive rates were significantly higher. The histological rate per thousand for severe dysplasia increased from 0.60 to 2.09, and for carcinoma in situ from 0.35 to 1.36. All six invasive carcinomata were detected in the GP programme (0.31%). The general practitioner is clearly more able to attract the 'high-risk' groups.

Adult↗

The reproducibility of cytomorphometrical grading of bladder tumours.

The reproducibility of cytomorphometrical nuclear area measurements on transitional cell carcinoma is studied. The inter-individual consistency is low (r = 0.55, P = 0.0005) when nuclei for measurement are chosen at random. When we use a systematic analysis based on cell situation and cytological features of malignancy to select the 50 nuclei for measurement per slide, the consistency between two technicians appears to be significantly higher (r = 0.90, P = 0.015). Not only interindividual consistency increases using the selection, but the correlation with histological tumour grade also improves significantly for both technicians. The results show that cytomorphometry is a method of grading bladder carcinoma when an accurate selection of nuclei to measure is used. Cytomorphometrical studies should contain a clear description of the way the nuclei for measurement are chosen.

Humans↗

Effect of regular 3-yearly screening on the incidence of cervical smears: the Leiden experience.

The effect of regular 3-yearly screening over a period of 12 years, on the incidence of cervical cancer in 25,000 women aged 35-54 years, is examined. The rate of squamous cell carcinoma decreased from 0.38 per 1000 women in the first round to zero in the fourth round. Similarly, the rate of carcinoma in situ declined from 1.69 per 1000 women in the first round to 0.35 per 1000 in the fourth round. The rates of severe dysplasia showed no decline. Cytologic under diagnosis of carcinoma-in-situ and cervical carcinoma increased with each round. Widowed and divorced women and those living in urban areas were identified as high risk groups.

Adenocarcinoma↗

Staining of the nucleolus with protein and RNA stains for automatic measurement of nucleolar size in paraffin sections.

In this paper two staining methods for automatic assessment of nucleolar profile area in paraffin sections of breast carcinomas using the IBAS 2000 image analyzer are reported. In the first method, the nucleolar proteins are stained with phloxine B, in the second the nucleolar RNA is stained with methyl green-pyronin. Both methods are fully reproducible. Statistically, the profile areas observed for the same patient by the two methods were found not to differ significantly. Because of their rigidity and their size in relation to section thickness, nucleoli are particularly attractive cell structures for quantitation, for instance in studies of variables that might be useful in establishing the prognosis for carcinoma patients.

Breast Neoplasms↗