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Biomedical subjects

A Wikby

Publications and source records attributed to A Wikby.

23 records · Page 2Linked to original sources
PubMed1980

High-performance liquid chromatographic determination of epimers, impurities, and content of the glucocorticoid budesonide and preparation of primary standard.

The new glucocorticoid budesonide, a 1:1 mixture of two epimers, was evaluated analytically with respect to drug content, impurities, and epimer distribution in the drug substance. Reversed-phase high-performance liquid chromatographic (HPLC) systems using octadecylsilane columns with different ethanol-water mixtures as eluents were used. Budesonide and its epimers were determined with recoveries of 100.3% (+/- 0.16 SD) and 99.7% (+/- 0.27 SD), respectively. Individual foreign steroids in the ranges of 0.05--0.2 and 0.2--2% were determined with mean recoveries of 93% (+/- 7.6 SD) and 97% (+/- 2.5 SD), respectively. Analysis of eight small-scale production batches of budesonide gave a recovery of 99.9% (+/- 0.39 SD) for the sum of budesonide and impurities. Analytical data and the procedure for preparation of a budesonide primary standard are given. The proposed HPLC procedure is faster, more accurate, more precise, and more selective than the usual pharmacopeial methods for corticosteroids.

Budesonide↗
PubMedDate not supplied

Insulin-pen treatment, quality of life and metabolic control: retrospective intra-group evaluations.

The significance of the insulin pen for the quality of life of patients with insulin-dependent diabetes mellitus (IDDM) has been debated. The aim of this study was to empirically investigate whether quality of life and metabolic control improve and whether insulin requirements change subsequent to multiple injection-pen treatment. The study group comprised 72 consecutive outpatients with IDDM. Thirty-eight subjects had an initial daily regimen of one or two injections, and the remaining 34 subjects had three or more injections. All patients had four or five injections per day during pen treatment. Perceived changes in quality of life attributed to pen treatment were assessed retrospectively at follow-up 9-13 months after the changeover. Data on metabolic control (HbA1c) and insulin dose were collected at base-line and follow-up. The life quality of the IDDM-patients improved consistently, a finding corroborated by recent studies with other designs and methods. Metabolic control improved only for those patients who previously had one or two injections. The insulin requirements did not change. In conclusion, the pen contributes to a better life for the IDDM patient. The quality of life changes due to treatment intervention appear to be assessable.

Adult↗
PubMedDate not supplied

Age-related changes in immune parameters in a very old population of Swedish people: a longitudinal study.

This study used a longitudinal design to examine age-related changes in a well-defined sample of Swedish people ranging from 86 to 92 years of age at baseline. The longitudinal design encompassed three measurement occasions with 1 year intermeasurement intervals. The results were analyzed by multivariate analyses of variance (MANOVA), which is useful for comparing individuals over time. Healthy middle-aged subjects (39 years SD +/- 5.8) served as controls. The proliferative responses to Concanavalin A (Con A), a T-cell mitogen, indicated significant lower levels in responses of the old when the two groups were compared. The MANOVA revealed no significant change in mitogen responses over measurement occasions in the old sample as compared with the young. However, when cell types and lymphocyte subpopulations were examined, significant differences were found between the two age groups in many of these parameters and for some (lymphocyte percentages and numbers, CD3 numbers) the MANOVA indicated significant decreases over the measurement occasions in the very old. The results also consistently indicated significant intraindividual correlations in cell types, lymphocyte subpopulations, and mitogen responses over time.

Adult↗
PubMedDate not supplied

Metabolic control, quality of life, and negative life events: a longitudinal study of well-controlled and poorly regulated patients with type 1 diabetes after changeover to insulin pen treatment.

In a previous study of a group of 74 patients with Type I diabetes, quality of life was found to be consistently enhanced a year after transition to multiple injection therapy with the insulin pen, whereas metabolic control (HbA1C) only improved moderately. The aim of the present investigation was to examine quality of life, recent life events, and metabolic control longitudinally in this original study group over a 5-year period beginning 1 year after transition to the insulin pen. Multiple analysis of variance with a repeated-measures design was used to analyze the data longitudinally and compare metabolic control in subgroups of well-controlled and poorly regulated patients during the study period. For the group as a whole, quality of life was found to change only moderately, whereas metabolic control deteriorated significantly across time following transition to the insulin pen. The two subgroups exhibited distinct differences, however, in quality of life, recent life events, and metabolic control patterns. These findings are discussed in terms of the clinical suitability of a multiple injection regimen.

Aged↗