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Biomedical subjects

A Yabuki

Publications and source records attributed to A Yabuki.

26 records · Page 2Linked to original sources
PubMed1982

[Experimental and clinical studies of cefmenoxime in the field of obstetrics and gynecology].

The study group was organized to evaluate the usefulness of cefmenoxime (CMX) injection, a new synthetic cephalosporin, for the treatment of infections in the field of obstetrics and gynecology. Fundamental and clinical studies were made by the society and the following results were obtained. 1. The peak distribution of CMX's MIC for E. coli, Klebsiella sp., Enterobacter sp., Bacteroides sp. and Peptococcus sp. isolated from obstetrical and gynecological infections with relatively high frequencies area 0.1, less than or equal to 0.05, 0.2, 3.13, 1.56 micrograms/ml, respectively, with an inoculation of 10(6) cells/ml. 2. When 1 g of CMX is administered by intravenous drip infusion for 1 hour, the maximum concentrations in various tissues of female genital organs were as follows: 14.2 and 13.2 micrograms/g in ovary and oviduct, respectively, at 1.20 hours after the start of administration, and 16.9 and 26.3 micrograms/g in corpus uteri and cervix uteri, respectively, after 1 hour. As for the transfer to the exudate in the pelvic dead cavity, the peak concentration was 15.6 micrograms/ml after 2.13 hours. 3. In the clinical studies, CMX was given to 258 cases with female genital organ infections and others. As for the clinical effects, with exclusion of 3 cases in which other antibiotics are concomitantly used, responses were excellent in 76 cases, good in 162 cases and poor in 17 cases, among 255 cases in total. The efficacy rate was 93.3%. The efficacy rates by diseases were 97.1% (68/70) for intrauterine infections, 88.8% (79/89) for intrapelvic infections, 98.4% (62/63) for adnexitis, and 100% (23/23) for infections of external genital organs. As for the clinical effects on causative bacteria, the efficacy rates were 100% (19/19) for single infections due to Gram-positive bacteria, 94.8% (55/58) for single infections due to Gram-negative bacteria, and 88.2% (15/17) for single infections due to anaerobic bacteria. And its efficacy rates were 89.6% (69/77) for mixed infection cases. Side effects were observed in 2 cases (0.8%); 1 case with eruption, and 1 case with diarrhea and vomiting. As for abnormal laboratory findings, lower white blood cell count was observed in 2 cases and elevation of the values regarding hepatic functions in 9 cases. All cases were returned to the normal after the completion of the administration. Cefmenoxime showed a satisfactory clinical efficacy and a potent bacteriological effect in treatment of the infections in the field of obstetrics and gynecology, and it has been concluded that cefmenoxime will be useful addition to the antibiotics for the therapy of these infections.

Adolescent↗
PubMedDate not supplied

Characterization of a pyridoxalated hemoglobin-polyoxyethylene conjugate as a physiologic oxygen carrier.

A pyridoxalated hemoglobin-polyoxyethylene conjugate (PHP) is an oxygen carrier prepared from outdated human red cells through chemical modifications of hemoglobin (Hb). PHP was evaluated as a physiologic oxygen carrier. PHP was synthesized from a pyridoxalated hemoglobin (PLP-Hb) and an activated diester of polyoxyethylene (POE). The average numbers of molecules of PLP and POE conjugated to a Hb molecule were 3.3 and 6.2, respectively. The p50 of PHP was 20.4 torr, which was much higher than the 14.0 torr of stroma-free Hb. At a Hb concentration of 6 g per dL, PHP had a physiologic colloid osmotic pressure (29 torr) and a lower viscosity (2.6 cP at 37 degrees C) than whole blood (4.2 cP at 37 degrees C). At temperatures below 20 degrees C, the p50 of PHP was higher than that of the red cells. PHP, which had an average molecular weight of 90,000, consisted of 83 percent monomeric PLP-Hb-POE conjugate, 12 percent dimeric PLP-Hb-POE, and 4 percent trimeric PLP-Hb-POE. The consistency of the relative contents of the components was well controlled through seven lots, and each component had sufficient oxygen-carrying capacity. The dimeric and trimeric components contributed to the prolongation of the residence of PHP in the circulation. The results indicate that PHP has favorable properties as an oxygen-carrying blood substitute and perfusate.

Animals↗
PubMedDate not supplied

Renal effects of a pyridoxalated-hemoglobin-polyoxyethylene conjugate solution as a blood substitute in exchange transfusions.

With PHP as an oxygen carrier, histologic studies in ET showed changes of renal tubular epithelial cells at 2 weeks post ET, with normal structure by 3-12 months post ET. The renal functional effects of PHP were evaluated by ET (30%, N = 3; 50%, N = 1; 80%, N = 3) in seven healthy mongrel dogs. Blood, urine, and renal biopsy specimens were taken pre ET and at 0, 1, and 2 days, 2, 4, and 6 weeks, and 3, and 6 months post ET. Data were compared to modified criteria of acute tubular necrosis. All dogs tolerated the procedure well and survived for 1 year. Urine output was normal with elevation during the first 2 days in the 50 and 80% ET, followed by stable output by 2 weeks, ranging from 12 to 60 ml/kg/day. Blood urea nitrogen (BUN) and serum creatinine (SCr) were normal. BUN/SCr was normal. The urine to plasma osmolality ratios were 2.6 to 8.3 (normal greater than 1), and fractional percent excretions of sodium (FES) were stable throughout. No existence of broad granular pigmented casts (BGPC) in urine sediment were noted. Renal histologic evaluation of vacuolization in the renal tubules were seen to be dose-dependent and transient, with normal histology by 3-6 months post ET. Dose-dependent vacuole formation observed in the early weeks post ET with PHP showed no renal functional changes. Based upon the modified criteria for acute tubular necrosis, no histologic abnormalities were noted.

Animals↗
PubMedDate not supplied

In vitro evaluation of a pyridoxalated hemoglobin polyoxyethylene conjugate in reversing cell sickling.

In sickle cell disease (SCD) microcirculatory blockage by red blood cells (RBC) occurs because of their low oxygen concentration, which results in both sickling and painful crises. Pyridoxalated hemoglobin polyoxyethylene (PHP), developed from human RBC hemoglobin (Hb) by chemical modification as an oxygen carrier, was evaluated in vitro for its ability to reverse cell sickling. PHP solutions of 6 or 8 g % Hb and a P50 of 20 mmHg were evaluated. RBC were obtained from SCD patients treated by exchange transfusion. The in vitro positive pressure filtration method (47 mm 5 microns Nuclepore membrane) was used. Comparisons of PHP with low oxygen carrier solutions (Hespan, saline) were made at flows of 0.43 to 6.0 ml/min. PO2 increases in a 20% mixture of air saturated solutions with deoxygenated sickle cell suspensions (SCS), at a hematocrit of 1%, were significantly higher in PHP as compared with saline and Hespan. The filtration resistance of deoxygenated SCS mixed with PHP was significantly lower than that of deoxygenated SCS mixed with saline and Hespan, and was comparable to that of air oxygenated SCS. By the 20% (v/v) addition of air saturated PHP to deoxygenated SCS, 89 +/- 2% of the sickled cells were unsickled. A novel artificial capillary system (ACS) modeling the dynamics of the microcirculation of the body was used. With the ACS plugged with deoxygenated cells, perfusion with oxygenated cell-PHP solutions was significantly more efficient in reversing the blockage than oxygenated saline and Hespan solutions. PHP reverses cell sickling by its effective delivery of oxygen.

Anemia, Sickle Cell↗