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A Yacoub

Publications and source records attributed to A Yacoub.

25 records · Page 2Linked to original sources
PubMed1990

Detection of Staphylococcus aureus infection by enzyme-linked immunosorbent assay and immunoblotting, using high molecular weight staphylococcal proteins.

Two high molecular weight staphylococcal proteins, fibronectin-binding protein and a Mr 200,000 protein, were investigated as antigens for serodiagnosis of staphylococcal infections. Sera from patients with staphylococcal infections and from controls were subjected to immunoblot analysis with staphylococcal lysate proteins to identify staphylococcal antigens to which patients with staphylococcal infections specifically exhibited antibodies. One such protein was found in the Mr 200,000 region. This protein was purified and used as antigen in ELISA and compared with other antigens, namely fibronectin-binding protein(s) (FNBP, Mr 185,000), alpha-toxin and teichoic acid. Sera from patients with staphylococcal infections contained antibodies to the high molecular weight proteins in higher titers than sera from patients with non-staphylococcal infections or healthy subjects. Based on their amino-acid compositions and different abilities to bind fibronectin it was concluded that the Mr 200,000 protein and FNBP were not identical.

Adolescent↗
PubMed1987

Juvenile hyaline fibromatosis: two new patients and review of the literature.

We report on a sister and a brother (born to normal consanguineous parents) with joint contractures and osteolytic lesions of bones. The sister had also gingival hyperplasia and skin lesions consisting of multiple tumors of the face, nose, palate, ears, and neck. Histologic examination showed findings of juvenile hyaline fibromatosis. The literature is reviewed, and 15 cases already reported are summarized.

Child, Preschool↗
PubMed1987

The epidemiology of schistosomiasis in the later stages of a control programme based on chemotherapy: the Basrah study. 1. Descriptive epidemiology and parasitological results.

The association between infection with Schistosoma haematobium and various factors including water contact pattern, past history of haematuria and treatment for schistosomiasis and cercarial dermatitis was investigated in Southern Iraq. The study covered mainly primary schoolchildren in different areas of Basrah and in Al-maadan locality where the majority of households with infected schoolchildren occurred. Urine examination was by the sedimentation and Nuclepore filtration methods. S. haematobium infection was very focal in distribution. There was evidence of local transmission in the Al-maadan locality, while in Abu-al-Jawzi schistosomiasis might have been acquired from endemic areas outside Basrah governorate. Swimming, history of haematuria, and history of treatment of schistosomiasis were significantly associated with the level of infection determined by egg count. Cercarial dermatitis, and bathing, washing clothes and fetching water were not significantly associated. The prevalence of infection among male members of households of infected children was significantly higher than that among male members of the remaining households in Al-maadan. There was no evidence of clustering of cases at household level. The implications of these findings for control and for future research on schistosomiasis in Basrah are discussed.

Adolescent↗
PubMed1987

The epidemiology of schistosomiasis in the later stages of a control programme based on chemotherapy: the Basrah study. 2. The serological profile and the validity of the ELISA in seroepidemiological studies.

An epidemiological study of Schistosoma haematobium infection in Al-maadan locality, considered to be the main remaining endemic focus in Basrah, southern Iraq was carried out. The association between the serological profile of the population, measured by the enzyme linked immunosorbent assay (ELISA), and various factors including current infection by urine examination, water contact pattern, past history of, and treatment for, schistosomiasis, and cercarial dermatitis was investigated. Further study of the serological data by the relative operating characteristic (ROC) analysis to assess the validity of the ELISA in detecting schistosomal infection, showed that current infection, past history of infection, age, cercarial dermatitis and category of household were significantly associated with the serological profile of the population. The analysis allowed quantification of the effects of past history of infection and cercarial dermatitis on the validity of the ELISA in detecting schistosomiasis.

Adolescent↗
PubMed1987

The epidemiology of schistosomiasis in the later stages of a control programme based on chemotherapy: the Basrah study. 3. Antibody distributions and the use of age catalytic models and log-probit analysis in seroepidemiology.

A comparative seroepidemiological survey of Schistosoma haematobium infection among primary schoolchildren in areas of different levels and patterns of endemicity in Basrah governorate, southern Iraq, also covered all inhabitants of Al-maadan locality, a known endemic focus in Basrah. Blood samples were tested by the enzyme linked immunosorbent assay. Serological data were analysed to describe the pattern of distribution of antischistosomal antibodies, to relate the level of infection to age and sex, and to estimate the force of infection per year by applying age catalytic models. The distributions of antibodies among the study populations were positively skewed; 3 patterns were revealed by relating serological data to age and sex, depending on the level of infection. The simple catalytic model was the best to estimate the force of infection among the younger age groups.

Adolescent↗
PubMedDate not supplied

Uptake of porcine rubulavirus (LPMV) by PK-15 cells.

BACKGROUND: The porcine virus denominated La Piedad Michoacan Virus (LPMV) is a member of the family Paramyxoviridae and is the cause of a disease in pigs present only in Mexico. The disease is characterized by meningoencephalitis and respiratory distress in young pigs, epididymitis and orchitis in boars, and reproductive failure and abortion in sows. METHODS: The cytopathology, morphology, and distribution of the hemagglutination neuraminidase (HN) and nucleoprotein (NP) proteins of LPMV were investigated following inoculation into PK-15 cells. The cytopathic effect was characterized by cytoplasmic vacuolation and the formation of syncytia and cytoplasmic inclusion bodies. RESULTS: In immunofluorescence assays using a monoclonal antibody (MAb) against the HN protein at 5-60 min post-infection (early infection), a diffuse immunofluorescence was observed near the cell membrane and adjacent to the nuclear membrane. At 24 h post-infection (late infection), a dust-like immunofluorescence was observed throughout the cytoplasm. LPMV-infected cells incubated with the MAb against the NP protein showed punctate cytoplasmic fluorescence during the early stages of infection. At the late infection stage, these fluorescent particles became larger and were seen predominantly in the cytoplasm of syncytia. This pattern was also apparent by immunohistochemical labeling and immunogold electron microscopy. The latter technique revealed that HN protein was diffusely distributed throughout the cytoplasm. When using the MAb against the NP protein, nucleocapsid organization was the most prominent feature and resulted in the formation of cytoplasmic inclusion bodies visible by light and electron microscopy. Immunogold labeling of purified nucleocapsids was shown by electron microscopy. Virus particles and nucleocapsids were morphologically similar to members of the Paramyxoviridae family. CONCLUSIONS: The morphologic characteristics of the virions and the distribution patterns of the HN and NP proteins in PK-15 infected cells indicate that the mechanisms of LPMV replication are generally similar to those of the members of the Paramyxoviridae family.

Animals↗