Biomedical subjects
Publications and source records attributed to A Zekri.
Accumulation of bone seeking radiopharmaceuticals in soft tissue of the chest has many causes. We report two cases of 99m-Tc-HMDP uptake respectively in the lung, in a patient with localized amyloidosis, and on diaphragmatic metastasis of a PNET. Mechanisms of such uptake are discussed.
The objective of this study was to determine whether microsatellite instability (MSI) and loss of heterozygosity (LOH) are involved in the immortalization of human breast epithelial cells (HBECs) in vitro and in the early stages of their transformation by benzo[a]pyrene (BP) and 7,12-dimethylbenz[a]anthracene (DMBA). We performed a genome-wide analysis of a total of 466 microsatellite DNA polymorphism loci along the X chromosome and the 22 pairs of human autosomes. MSI was found in the immortalized MCF-10F cells at the following loci: D11S1392 (on chromosome 11p13) and D17S849 (at 17p13.3), D17S796 (at 17p13.1), D17S513 (at 17p13.1), TP53 (at 17p13.1), D17S786 (at 17p13.1), and D17S520 (at 17p12) on chromosome 17. The BP-transformed cells exhibited MSI in the same loci and also in locus D11S912 (at 11q25). The more transformed BP1E cells also exhibited MSI on chromosome 13q12-13 at D13S260 and D13S289, markers known to flank the breast cancer susceptibility gene BRCA2. In the DMBA-transformed D3 and D3-1 cells, MSI was observed at the locus D13S260 in addition to the previously reported locus D16S285 (at 16q12.1). No LOH was observed on any of the chromosomes tested in these cells. These observations led us to conclude that the immortalization and transformation of HBECs may involve defects in mechanisms responsible for the cell's genomic stability, such as DNA replication and DNA mismatch repair.
Human breast cancer remains the most common malignancy in the American women. The ultimate cure of this disease relies on a better understanding of the mechanisms underlying the initiation and progression of this disease. The neoplastic transformation of HBEC in vitro represents a successful model for obtaining knowledge on the molecular and biological alterations that may contribute to the tumorigenic mechanisms. We have presented here a current understanding of chemically transformed HBEC in the following aspects: 1. Factors affecting the transformation of HBEC such as genetic predisposition and differentiation status and prior immortalization; 2. New targets for studying the mechanism of cell immortalization such as alterations in telomerase activity and differential expression of cell cycle dependent genes as well as others recently isolated through differential cloning such as H-ferritin, and a calcium binding protein; 3. Epigenetic and genetic mechanisms underlying cell transformation; 4. The association of microsatellite instability in specific loci on chromosomes 11, 13, and 16 with the progression of cell transformation; and 5. The application of microcell mediated chromosome transfer technique as an approach to testing the functional role of specific genes whose dysregulation or loss of function may contribute to the ultimate cell transformation. Further efforts in this cell system will be directed to determine the roles of identified molecular changes as well as the mapping/cloning of tumor suppressor or senescence genes such as those that may reside on chromosomes 11 or 17.
Ultrasonic ablation of tissue by the surgical ultrasonic aspirator (SUA) is an established technique in neurosurgery and hepatobiliary surgery. We report the first use of SUA in the debridement of burn wound in three patients. Our initial experience suggested that SUA allows meticulous wound debridement and cleansing with minimal bleeding and destruction of normal tissue and, therefore, is an extremely useful surgical instrument to add to the armamentarium of the burn surgeon.
The injection of a high viscosity fluids into the tissues for cosmetic body contouring has been practised in the last four decades in the East and South-East of Asia. The injection of liquid paraffin for mammary augmentation was widely practised by surgeons, physician and even non medical people. Unfortunately, most of these cases ended by having different varieties of paraffinoma as a complication of a foreign body reaction. We report three cases of a destructive form of these paraffinomas ulcerating into both breasts and the anterior chest wall. One case was treated by bilateral mastectomy, radical excision of the anterior chest wall soft tissue and reconstruction by a vertical Rectus Abdominus Myocutaneous Flap. The second case had bilateral mastectomy and followed up for facial paraffinomas. The third case was just followed for up regular wound care as surgery was not indicated due to advanced age, poor general condition and the family request.
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A wide consensus exists today in favour of breast saving procedures for the surgical treatment of small breast cancers. The goal of conservative treatment is to preserve the shape of the breast and consequently to avoid the psychological consequences of the mutilation. In this respect, the final esthetic evaluation of conservative treatment is a major point to be considered after the oncological results. To evaluate these cosmetic results, 89 patients treated more than five years ago were analyzed on a standard picture protocol and rated according to a rigorous scale previously defined. In this group, 45% were rated as excellent, 34% as fair and 21% as poor. These results were compared to the cosmetic results of a second group of patients evaluated after the surgical stage of their conservative treatment in order to precise the morphological damage due to the surgical procedure. In the second group, 73% were rated as excellent, 20% as fair and 7% as poor. This study underlines the frequency of skin contractions (16%) and glandular defects (20%). Several rules are proposed to prevent such sequelae at the time of the tumorectomy. The difficulty of cosmetic improvement of the poor esthetic results is emphasized and the techniques of partial reconstructions are discussed in the light of our experience which includes 25 cases of partial reconstruction.
From 1976 to 1986 one hundred and twenty immediate breast reconstructions (IBR) were performed at the Gustave-Roussy Institut. 90% of these cases were treated for in situ or infiltrating carcinomas, while only 10% had prophylactic mastectomy. Over recent decades indications for IBR have progressively changed. In the first period they were reserved for prophylactic mastectomies and in situ carcinomas. Over the last several years IBR has also been proposed to patients with infiltrating carcinomas (30%). Although the adequate treatment for small invasive carcinomas is a radiosurgical conservative procedure, a mastectomy is still indicated in stage II, over three centimeters multifocal carcinomas, and local recurrence after conservative treatment. In our series, this procedure seems safe and advisable as it does not interfere with the treatment of primary and secondary disease. The low incidence of surgical complications reported in our series did not increase the hospital stay of our patients. Indications, surgical techniques including myocutaneous flaps, and results of IBR will be discussed.