Biomedical subjects

Adam M Fermier

Publications and source records attributed to Adam M Fermier.

4 recordsLinked to original sources

PubMed2006

An automated mobile phase preparation workstation.

An automated solvent dispensing workstation capable of delivering volumes ranging from 10 mL to 4.5 L for the preparation of solutions/mobile phases was developed and implemented into the industrial R&D laboratory. The workstation was designed to address business, safety, and compliance needs while meeting or exceeding the precision and accuracy of current manual methods of preparation. The system's performance was optimized with respect to liquid transfer tubing inner diameter, pumping pressure, flow characteristics of the valve, and computer control logic. The automated solvent dispensing workstation was shown to exceed the specifications set by the ASTM for Class A graduated cylinders for all dispense volumes (10 mL-4.5 L).

Automation↗

PubMed2004

Very high pressure HPLC with 1 mm id columns.

Theoretical calculations and experimental data indicate that very high pressure HPLC can be performed using 1 and 1.5 mm id columns, and contrary to previous beliefs, the frictional heating generated does not appear to be detrimental to the separation.

Chromatography, High Pressure Liquid↗

PubMed2003

Powder dispensing robot for sample preparation.

An automated powder dispensing station capable of transferring milligram quantities (1-100 mg) of powder for sample preparation was developed and integrated into a commercial robotic workstation (Zymark Prelude). The system's performance was optimized with respect to vacuum flow rate and powder transfer tube cross sectional area, and shown to possess excellent powder dispensing accuracy (RSD = < or = 0.1% for target weights < or = 15 mg) and precision (RSD = 3.43%) for a vanillin sample. Using the commercial features of the Zymark Prelude workstation (liquid handling, weighing, and vortexing/mixing) and the custom powder dispensing station, multiple sets of analytical calibration standards were prepared and subsequently analyzed by FIA in order to assess the system's robustness for sample preparation.

Drug Industry↗

PubMed2002

Comparing cyclodextrin derivatives as chiral selectors for enantiomeric separation in capillary electrophoresis.

A total of 26 different cyclodextrin (CD) derivatives with different functional groups and degrees of substitution were tested against 35 basic pharmaceutical compounds in an effort to investigate their effectiveness as chiral selectors for enantiomeric separation in capillary electrophoresis (CE). Testing was performed under the same conditions using a low pH buffer (25 mM phosphate buffer at pH approximately 2.5). Five CD derivatives, namely, highly sulfated-beta-CD, highly sulfated-beta-CD, hydroxypropyl-beta-CD (degree of substitution approximately 1), heptakis-(2,6-O-dimethyl)-beta-CD, and heptakis(2,3,6-O-trimethyl)-beta-CD were identified to be most effective for enantiomeric separations and have a wide range of enantiomeric selectivity towards the model compounds. Over 90% of the model compounds were enantiomerically resolved with the five identified CD derivatives, at a minimum resolution of 0.5. An additional 20 compounds were also tested to demonstrate the validity of the identified CD derivatives. The five CD derivatives were recommended as the starting chiral selectors in developing enantiomeric separation methods by CE.

Cyclodextrins↗