Biomedical subjects

Adam S G Curtis

Publications and source records attributed to Adam S G Curtis.

23 records · Page 2Linked to original sources

PubMed2003

Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography.

Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new tissue. Material surface morphology has strong effects on cell cytoskeleton and morphology, and it is thought that cells may react to the topography of collagen and surrounding cells during tissue embryology. A poorly understood area is, however, gene-level responses to topography. Thus, this paper used microarray to probe for consistent gene changes in response to lithographically produced topography (12.5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling.

Cell Adhesion↗

PubMed2003

Dextran and albumin derivatised iron oxide nanoparticles: influence on fibroblasts in vitro.

Magnetic nanoparticles have been used for biomedical purposes for several years. In recent years, nanotechnology has developed to a stage that makes it possible to engineer particles to provide opportunities for the site-specific delivery of drugs. To this end a variety of iron oxide particles have been synthesised. The size and surface of the particles are crucial factors in the application of the particles. This study therefore involves the use of magnetic nanoparticles synthesised and derivatised with either dextran or albumin, compared to identical underivatised plain particles. This influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell-particles interaction, including light and fluorescence microscopy, scanning and transmission electron microscopy. The results indicate that the derivatised particles induce alterations in cell behaviour and morphology distinct from the plain particles, suggesting that cell response can be directed via specifically engineered particle surfaces.

Albumins↗

PubMed2002

Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands.

It is becoming clear that cells can respond not only to micometric scale topography, but may also to nanometric scale topography. The production of reproducibly sized nanometric features has relied heavily on expensive and time-consuming methods of manufacture, such as electron beam lithography. Polymer demixing of polystyrene and polybromostyrene has been found to produce nanoscale islands of reproducible height, and the islands have been previously shown to effect cell spreading compared to planar surfaces. This study observes morphological, cytoskeletal, and molecular changes in fibroblast reaction to 13-nm-high islands. The methods employed include scanning electron microscopy, fluorescent microscopy, and 1718 gene microarray. The results show that the cells respond to the islands by broad gene up-regulation, notably in the areas of cell signaling, proliferation, cytoskeleton, and production of extracellular matrix proteins. Microscopical results provide confirmation of the microarray findings.

Biocompatible Materials↗

PubMed2002

The influence of elastin-coated 520-nm- and 20-nm-diameter nanoparticles on human fibroblasts in vitro.

Magnetic nanoparticles have been used for biomedical purposes for several years. In recent years, nanotechnology has developed to a stage that makes it possible to engineer particles to provide opportunities for the site-specific delivery of drugs. To this end, a variety of iron oxide particles have been synthesized. The size and surface of the particles are crucial factors in the application of the particles. Therefore, this study involves the use of two types of magnetic nanoparticles derivatized with elastin and synthesized with differing diameters, compared with identical underivatized plain particles. This influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell-particle interaction, including light and fluorescence microscopy and scanning electron microscopy. The results indicate that derivatized particles induce alterations in cell behavior and morphology distinct from the plain particles, suggesting that cell response can be directed via specifically engineered particle surfaces. However, little difference was observed between the different diameters.

Cell Proliferation↗

PubMed2002

The NanoBioWorld.

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Biological Science Disciplines↗