Biomedical subjects

Adrian Gh Podoleanu

Publications and source records attributed to Adrian Gh Podoleanu.

7 recordsLinked to original sources

PubMed2006

Combined confocal/en face T-scan-based ultrahigh-resolution optical coherence tomography in vivo retinal imaging.

Combined confocal scanning ophthalmoscopy/en face T-scan-based ultrahigh-resolution optical coherence tomography (OCT) of the human retina in vivo is reported for the first time to our knowledge. The system uses a superluminescent diode-based broadband source, which gives an axial resolution of 3.2 microm in the retina. We demonstrate acquisition of T-scan-based OCT B-scan and simultaneous confocal/C-scan images of the human retina of large lateral size (covering a field of up to 20 degrees ) at a frame rate of 2Hz.

Equipment Design↗

PubMed2005

Fast scanning transmissive delay line for optical coherence tomography.

A novel Fourier domain fast scanning optical delay line is proposed in which the walk-off is eliminated by only two passes through a diffraction grating. Working in transmission, the novel delay line is ideal for balanced optical coherence tomography configurations with recirculation of the reference beam. We evaluate theoretically and experimentally its walk-off and dispersion compensation capabilities.

Equipment Design↗

PubMed2005

Simultaneous optical coherence tomography--Indocyanine Green dye fluorescence imaging system for investigations of the eye's fundus.

We have developed a dual-channel optical coherence tomography-Indocyanine Green dye (OCT-ICG) fluorescence system based on a previously reported ophthalmic OCT confocal imaging system. The confocal channel is tuned to the fluorescence wavelength range of the ICG, and light from the same optical source is used to generate the OCT image and to excite the ICG fluorescence. The system enables the clinician to visualize simultaneously en face OCT slices and corresponding ICG angiograms of the ocular fundus, displayed side by side. C-scan (constant depth) and B-scan (cross section) images are collected by a fast en face scan (T scan). The pixel-to-pixel correspondence between the OCT and angiography images allows the user to capture OCT B scans precisely at selected points on the ICG confocal images.

Algorithms↗

PubMed2004

Limitation of the achievable signal-to-noise ratio in optical coherence tomography due to mismatch of the balanced receiver.

Owing to the limited spectral response of the fiber directional coupler used in a balanced optical coherence tomography configuration, the spectra are different in the two outputs. This affects unfavorably operation of the balanced photodetector unit. Excess photon noise makes a larger contribution than a directional coupler with a flat spectral response. A theoretical model is developed that shows that an optimum set of parameters may be defined to maximize the achievable signal-to-noise ratio. The model leads to a redefinition of the effective noise bandwidth, which takes into account the nonflat response of the directional coupler used. The model also predicts a limitation on the signal-to-noise ratio even when the stray reflectances in the interferometer are brought to zero.

Journal Article↗

PubMed2004

Sequential optical coherence tomography and confocal imaging.

We report a system capable of sequentially acquiring two en-face images of different depth resolutions. The two images are generated by use of different principles, optical coherence tomography (OCT) and confocal microscopy, and have depth resolutions, at present, of better than 20 microm and over 0.12 mm, respectively. The lower-depth-resolution image is ideal for target positioning before collection of stacks of en-face OCT images. Switching between the two types of image by flipping an opaque screen in the reference arm, coupled with self-adjusting gain operation of avalanche photodiodes in the receiver. We illustrate the usefulness of the system by imaging a leaf and an optic nerve in vivo.

Equipment Design↗

PubMed2004

Quantification of root caries using optical coherence tomography and microradiography: a correlational study.

PURPOSE: The use of transverse microradiography (TMR) to quantify the amount of mineral lost during demineralization of tooth tissue has long been established. In the present study, the use of an en-face Optical Coherence Tomography (OCT) technology to detect and quantitatively monitor the mineral changes in root caries was investigated and correlated with TMR. MATERIALS AND METHODS: We used an OCT system, developed initially for retina imaging, and which can collect A-scans, B-scans (longitudinal images) and C-scans (en-face images) to quantitatively assess the development of root caries. The power to the sample was 250 microW, wavelength lambda = 850 nm and the optical source linewidth was 16 microm. RESULTS: Both the transversal and longitudinal images showed the caries lesion as volumes of reduced reflectivity. Quantitative analysis using the A-scan (reflectivity versus depth curve) showed that the tissue reflectivity decreased with increasing demineralization time. A linear correlation (r = 0.957) was observed between the mineral loss measured by TMR and the percentage reflectivity loss in demineralized tissue measured by OCT. CONCLUSION: We concluded that OCT could be used to detect incipient root caries, and that the reflectivity loss in root tissue during demineralization, measured by OCT, could be related to the amount of mineral lost during the demineralization.

Analysis of Variance↗

PubMedDate not supplied

Combined multiplanar optical coherence tomography and confocal scanning ophthalmoscopy.

We demonstrate the clinical application of a multiplanar imaging system that simultaneously acquires en face (C-scan) optical coherence tomography (OCT) and the corresponding confocal ophthalmoscopic images, along with cross-sectional (B-scan) OCT at specifiable locations on the confocal image. The advantages of the simultaneous OCT and confocal acquisition as well as the challenges of interpreting the C-scan OCT images are discussed. Variations in tissue inclination with respect to the coherence wave surface alter the sampling of structures within the depth of the retina, producing novel slice orientations that are often challenging to interpret. We have evaluated for the first time the utility of C-scan OCT for a variety of pathologies, including melanocytoma, diabetic retinopathy, choroidal neovascular membrane, and macular pucker. Several remarkable new aspects of clinical anatomy were revealed using this new technique. The versatility of selective capture of C-scan OCT images and B-scan OCT images at precise points on the confocal image affords the clinician a more complete and interactive tool for 3-D imaging of retinal pathology.

Equipment Design↗