Biomedical subjects

Ahmad Assalia

Publications and source records attributed to Ahmad Assalia.

5 recordsLinked to original sources

PubMed2006

Comparative evaluation of gastrointestinal transit and immune response between laparoscopic and open gastrectomy in a porcine model.

Several reports have shown advantages of laparoscopic gastrointestinal (GI) procedures when compared with their open counterparts. The aim of this study was to assess the differences of GI transit and immune response between laparoscopic and open gastrectomy in a porcine model. Fourteen pigs were assigned to undergo partial gastrectomy, either by laparoscopy (lap group) or by laparotomy (open group). GI transit was assessed using 24 markers and measured until half of markers were evacuated. To assess immune response, we used delayed-type hypersensitivity skin antigen testing (DTH) with vaccine antigen. DTH was evaluated at 48 hours after the primary injection for induration. After 2 weeks, all animals underwent necropsy and were evaluated for adhesion formation using a scoring scale. Operation time was significantly longer in the lap group. The GI transit in the postoperative phase was significantly prolonged compared with the preoperative measured times. Postoperatively, the GI transit in the open group was significantly prolonged compared with the lap group. Immune response measured by DTH was better preserved in the lap group than in the open group. Adhesion formation was significantly less in the lap group. We concluded that laparoscopic gastrectomy resulted in faster bowel recovery and less immune suppression.

Animals↗

PubMed2005

Laparoscopic gastric bypass with silicone band in a pig model: prevention of anastomotic dilatation -- feasibility study.

BACKGROUND: Morbid obesity has become a major global health problem. Surgery remains the only effective treatment for patients with severe obesity, because diet reduction methods and pharmacologic agents have not resulted in long-term weight reduction. Gastric bypass (GBP) can provide adequate weight loss, but after some years, dilatation of the gastric pouch and outlet may lead to weight regain by allowing the patient to increase food intake. METHODS: 2 groups of 6 pigs underwent laparoscopic GBP. In the first group, a non-adjustable silicone band (Proring-band, IOC, Innovative Obesity Care, Saint Etienne, France) was positioned 1 cm proximal to the gastrojejunal anastomosis. In the second group, the device used to stabilize the gastric pouch was an adjustable silicone band (Mid-band, Medical Innovation Developpement, Villeurbanne, France). Weight loss, complications and histological reaction were evaluated after 3 months. RESULTS: Mortality rate was 25% (cardiac arrythmia in 2 pigs). Conversion rate was 25%. The positioning of the band was more difficult with the Mid-band because of its larger size and the presence of the catheter. The average weight change in the Proring group was 15.8 kg (3.5-25.1 kg), and in the Mid-band group was 12.0 kg (6.2-15.1 kg). Morbidity consisted of one intragastric migration of the Proring band into the gastrojejunal anastomosis, and one infection of the port in the Mid-band group treated by removal of the port and antibiotics. CONCLUSION: Use of silicone devices may be safe and effective in the prevention of pouch or outlet dilatation after GBP.

Anastomosis, Roux-en-Y↗

PubMed2004

Laparoscopic pancreatic surgery for islet cell tumors of the pancreas.

The experience with laparoscopic pancreatic surgery (LPS) in general, and pancreatic islet cell tumors (ICTs) in particular, is still limited. Because insulinoma is the most prevalent tumor and is mostly benign, single, and curable with surgical excision, it comprises most of the cases. Our experience with 17 cases (10 insulinomas, 2 gastrinomas, 1 nesidioblastoma, 4 nonfunctioning tumors) and those recorded in the literature (93 cases) show that laparoscopic surgery for small, solitary benign islet cell tumors located in the body and tail is feasible and safe and can result in rapid postoperative recuperation and a complication rate comparable or lower than that achieved with open surgery. It duplicates the success rate seen with conventional surgery regarding intraoperative localization and cure of disease. The main morbidity continues to be the occurrence of a fistula (18%), most often after enucleation, but the clinical course is benign in most instances. Preoperative imaging studies are required for localization, and the combined use of biphasic helical computed tomography and endoscopic ultrasonography (US) seems to be cost-effective. The use of laparoscopic US is an integral part of the laparoscopic procedure, and the information achieved is valuable for both confirming localization and decision making concerning the most appropriate surgical procedure. In cases of distal pancreatectomy, splenic salvage, preferably with preservation of splenic vessels, is feasible albeit more demanding and can be achieved in most cases.

Adenoma, Islet Cell↗

PubMed2004

Endoscopic parathyroidectomy.

This article describes the techniques of endoscopic and endoscopic assisted parathyroidectomy, with a special emphasis on the accumulated experience as well as the advantages and drawbacks of these novel techniques.

Endoscopy↗

PubMedDate not supplied

Topical treatment with oxaliplatin for the prevention of port-site metastases in laparoscopic surgery for colorectal cancer.

BACKGROUND: The development of port-site metastases following laparoscopic resection of various malignancies continues to be a disturbing issue for laparoscopic surgeons. Previous studies revealed promising results with oxaliplatin, a third-generation platinum compound, as a first-line treatment in advanced colorectal cancer. This study evaluates the effect of topical application of oxaliplatin on the development of port-site metastases in an experimental murine model. METHODS: Nineteen female BDIX rats (immunocompetent, 6 weeks old) underwent a sham laparoscopic operation after 1 x 10(7) viable rat colon carcinoma viable cells (LMCR) had been injected into their peritoneal cavities. Three trocars (1 central camera port and 2 additional lateral ports) were introduced into the abdomen, and a pneumoperitoneum was created with carbon dioxide. Ten minutes after LMCR, cells were injected into the peritoneal cavity, the 2 lateral trocars were removed and carbon dioxide insufflation was maintained for an additional 5 minutes to allow for tumor cell seeding. Oxaliplatin (0.198 mg/kg) was then topically applied to 1 trocar site intramuscularly, while the other site was left untreated. One week later, the animals were euthanized, and the port sites were histologically examined for evidence of metastases. RESULTS: The rate of tumor implantation at the muscle layer in control sites was 68% (13/19) compared with 37% (7/19) at oxaliplatin-treated sites (P = 0.1). Also, no significant differences were detected in port-site metastasis rates in other untreated layers of the abdominal wall. CONCLUSION: Intramuscular topical application of oxaliplatin may not decrease the incidence of port-site metastasis in a syngeneic animal model of colon cancer. Nevertheless, we can see the tendency of declination. Further studies are needed to better determine its possible therapeutic role in high-risk humans undergoing laparoscopic resection of colorectal malignancies.

Administration, Topical↗