Biomedical subjects

Alun G Williams

Publications and source records attributed to Alun G Williams.

11 recordsLinked to original sources

PubMed2006

No correlation between circulating ACE activity and VO2max or mechanical efficiency in women.

The insertion (I) variant of the angiotensin-1 converting enzyme (ACE) I/D genetic polymorphism is associated with lower circulating and tissue ACE activity. Some studies have also suggested associations of ACE I/D genotype with endurance phenotypes. This study assessed the relationships between circulating ACE activity, ACE I/D genotype, mechanical efficiency and the maximal rate of oxygen uptake in sedentary individuals. Sixty-two untrained women were tested for mechanical efficiency during submaximal cycle ergometry (delta and gross efficiencies during exercise between 40 and 80 W) and the maximal rate of oxygen uptake during incremental treadmill running. Respiratory variables were measured using indirect calorimetry. Venous blood was obtained for direct assay of circulating ACE activity, allowing for the assessment of correlations between two continuous variables, rather than a categorical analysis of endurance phenotype by genotype alone. ACE I/D genotype was also determined, and was strongly associated with circulating ACE activity (P < 0.0005). Neither circulating ACE activity (27.4 +/- 8.4 nM His-Leu-ml(-1)) nor ACE genotype showed a statistically significant association with any of the endurance phenotypes measured. The weak correlations observed included r = -0.122 (P = 0.229) for the relationship between delta efficiency (23.9 +/- 2.5%) and circulating ACE activity and r = 0.134 (P > 0.6) for the relationship between maximal aerobic power (149.1 +/- 22.9 ml kg(-2/3) min(-1)) and circulating ACE activity. The data do not support a role for systemic ACE activity in the regulation of endurance performance in sedentary individuals, extending this observation to a large female cohort.

Adult↗

PubMed2006

Methodological issues with the interpolated twitch technique.

A number of methodological issues in the use of the interpolated twitch technique were investigated for their effect on true maximum force (TMF) and activation (ACT): timing of control (pre- vs post-contraction) and superimposed twitches (first vs second); type of twitch stimulus (primarily magnitude); and the type of extrapolation utilised. On three occasions subjects performed a series of maximal and sub-maximal contractions of the knee extensors, with electrically evoked twitches delivered before, during and after each contraction. The twitch-voluntary force relationship was concave for all types of twitch stimuli, and extrapolation using this relationship typically calculated TMF 39N (7%) higher, and ACT 7% lower than linear extrapolation. The timing of the control (2-4%) and superimposed twitches (approximately 4%) both influenced TMF and ACT. Despite the different twitch stimuli being a range of magnitudes (13-32% maximum voluntary force) they did not affect TMF and ACT. A novel finding was that prior potentiation changed the shape of the twitch-voluntary force relationship. For precise measurement of TMF and ACT it is recommended that: extrapolation is based on the twitch-voluntary force relationship of the experimental model; and post-contraction potentiated twitches be used, as the superimposed twitch on a high level contraction appears to be potentiated.

Adult↗

PubMed2006

Influence of continuous and interval training on oxygen uptake on-kinetics.

PURPOSE: To examine the relative effectiveness of moderate-intensity continuous training and high-intensity interval training on pulmonary O2 uptake (VO2) kinetics at the onset of moderate- and severe-intensity cycle exercise in previously sedentary subjects. METHODS: Twenty-three healthy subjects (11 males; mean +/- SD age 24 +/- 5 yr; VO2peak 34.3 +/- 5.5 mL x kg(-1) x min(-1)) were assigned to one of three groups: a continuous training group that completed three to four sessions per week of 30-min duration at 60% VO2peak (LO); an interval training group that completed three to four sessions per week involving 20 x 1-min exercise bouts at 90% VO2peak separated by 1-min rest periods (HI); or a control group (CON). Before and after the 6-wk intervention period, all subjects completed a series of step exercise tests to moderate and severe work rates during which pulmonary VO2 was measured breath-by-breath. RESULTS: ANOVA revealed that continuous and interval training were similarly effective in reducing the phase II VO2 time constant during moderate (LO: from 31 +/- 8 to 23 +/- 5 s; HI: from 32 +/- 9 to 21 +/- 4 s; both P < 0.05; CON: from 30 +/- 6 to 29 +/- 7 s; NSD) and severe exercise (LO: from 35 +/- 6 to 24 +/- 7 s; HI: from 32 +/- 11 to 24 +/- 7 s; both P < 0.05; CON: from 27 +/- 7 to 25 +/- 5 s; NSD) and in reducing the amplitude of the VO2 slow component (LO: from 0.38 +/- 0.10 to 0.29 +/- 0.09 L x min(-1); HI: from 0.41 +/- 0.28 to 0.30 +/- 0.28 L x min(-1); both P < 0.05; CON: from 0.54 +/- 0.22 to 0.66 +/- 0.38 L.min; NSD). CONCLUSIONS: Six weeks of low-intensity continuous training and high-intensity interval training were similarly effective in enhancing VO2 on-kinetics following step transitions to moderate and severe exercise in previously untrained subjects.

Adult↗

PubMed2006

Effects of differential stretching protocols during warm-ups on high-speed motor capacities in professional soccer players.

The purpose of this study was to examine the effects of different modes of stretching within a pre-exercise warm-up on high-speed motor capacities important to soccer performance. Eighteen professional soccer players were tested for countermovement vertical jump, stationary 10-m sprint, flying 20-m sprint, and agility performance after different warm-ups consisting of static stretching, dynamic stretching, or no stretching. There was no significant difference among warm-ups for the vertical jump: mean +/- SD data were 40.4 +/- 4.9 cm (no stretch), 39.4 +/- 4.5 cm (static), and 40.2 +/- 4.5 cm (dynamic). The dynamic-stretch protocol produced significantly faster 10-m sprint times than did the no-stretch protocol: 1.83 +/- 0.08 seconds (no stretch), 1.85 +/- 0.08 seconds (static), and 1.87 +/- 0.09 seconds (dynamic). The dynamic- and static-stretch protocols produced significantly faster flying 20-m sprint times than did the no-stretch protocol: 2.41 +/- 0.13 seconds (no stretch), 2.37 +/- 0.12 seconds (static), and 2.37 +/- 0.13 seconds (dynamic). The dynamic-stretch protocol produced significantly faster agility performance than did both the no-stretch protocol and the static-stretch protocol: 5.20 +/- 0.16 seconds (no stretch), 5.22 +/- 0.18 seconds (static), and 5.14 +/- 0.17 seconds (dynamic). Static stretching does not appear to be detrimental to high-speed performance when included in a warm-up for professional soccer players. However, dynamic stretching during the warm-up was most effective as preparation for subsequent high-speed performance.

Acceleration↗

PubMed2006

Suitability of soccer training drills for endurance training.

Recent evidence suggests that certain soccer drills produce exercise intensities suitable for physical conditioning. However, it remains debatable whether soccer drills can provide a sufficiently unified exercise intensity among different players and on repetition of a drill, because movement patterns cannot be externally controlled during soccer drills. Good reliability and low variability of exercise intensity would enable all players to receive an appropriate training stimulus. The purpose of this study was to investigate intersubject variability and intrasubject reliability in exercise intensity during soccer drills. It was hypothesized that soccer drills that involve the highest exercise intensities would demonstrate the lowest intersubject variability and the highest intrasubject reliability. Heart rates of 23 professional soccer players were recorded during a range of soccer training drills. The drills consisted of 2 vs. 2 to 8 vs. 8 normal scoring games and 2 further possession games. Heart rate responses were examined for variability, reliability, and suitability for soccer endurance training. Coefficients of variation across players were less than 3% for all drills. Paired t-tests showed no significant differences in heart rate on repetition of the drills and 95% ratio limits of agreement were 1.8-3.8%. There were no significant correlations between exercise intensity and the statistical measures of variability and reliability. Several drills produced exercise intensities suitable for soccer endurance training with mean heart rate responses ranging from 87-91% HRmax. Soccer drills such as those used in the present study appear to be an adequate substitute for physical training without the ball and thus provide simultaneous skill and fitness training. The increase in training time spent developing technical ability and/or a reduction in total training time required may be useful for soccer teams.

Adult↗

PubMed2006

Can simple anthropometric and physical performance tests track training-induced changes in load-carriage ability?

The objective of this research was to determine whether tests of strength, endurance, and anthropometry could be used to assess training-induced changes (delta) in load-carriage performance. Eighty-four British Army recruits completed a 10-week training program. Strength, endurance, body composition, and 3.2-km load carriage with 15 kg and 25 kg were assessed before and after training. Training significantly increased load-carriage performance (7-16%), back extension and dynamic lifting strengths, endurance and fat-free mass, and reduced body fat. Statistically significant regression models (adjusted R2 of 0.063-0.797) were produced that predicted delta load-carriage performance. However, the 95% limits of agreement between observed and predicted delta load-carriage data showed margins of error (11-15%) comparable to the average training-induced gains in performance. Training-induced changes in load-carriage performance appear to be highly specific, which suggests that both training and testing procedures should also be specific to load-carriage performance.

Adolescent↗

PubMed2005

Specificity of acceleration, maximum speed, and agility in professional soccer players.

High-speed actions are known to impact soccer performance and can be categorized into actions requiring maximal speed, acceleration, or agility. Contradictory findings have been reported as to the extent of the relationship between the different speed components. This study comprised 106 professional soccer players who were assessed for 10-m sprint (acceleration), flying 20-m sprint (maximum speed), and zigzag agility performance. Although performances in the three tests were all significantly correlated (p < 0.0005), coefficients of determination (r(2)) between the tests were just 39, 12, and 21% for acceleration and maximum speed, acceleration and agility, and maximum speed and agility, respectively. Based on the low coefficients of determination, it was concluded that acceleration, maximum speed, and agility are specific qualities and relatively unrelated to one another. The findings suggest that specific testing and training procedures for each speed component should be utilized when working with elite players.

Acceleration↗

PubMed2005

Circulating angiotensin converting enzyme activity is correlated with muscle strength.

PURPOSE: The D-variant of the angiotensin-1 converting enzyme (ACE) gene is associated with higher circulating and tissue ACE activity. Some studies have suggested a similar association of genotype with muscle strength or the gain in strength in response to training. This study has assessed the relationship between circulating ACE activity, strength, and the response to training. METHODS: Eighty-one untrained men were tested for quadriceps muscle strength, and 44 of these performed an 8-wk program of dynamic strength training of the quadriceps muscle group. Venous blood was obtained for assessment of circulating ACE activity before and after the training program. ACE genotype was also determined. RESULTS: At baseline, circulating ACE activity was significantly correlated with isometric (r = 0.25-0.29, P < 0.02) and isokinetic (r = 0.38, P < 0.0005) quadriceps muscle strength. ACE genotype also seemed to be related to pretraining muscle strength. However, circulating ACE activity showed no significant association with the 9-14% mean increases of muscle strength in response to the training intervention. ACE genotype also showed no association with the training-induced change in muscle strength. Circulating ACE activity did not change significantly after the training program. CONCLUSIONS: The data support a role for ACE in the regulation of human skeletal muscle strength, but do not confirm a role in altering the response to short-term training.

Adolescent↗

PubMed2004

Training diagnosis for a load carriage task.

To explore the possibility of training diagnosis for a 3.2-km loaded march with a 25 kg load, 50 men trained for 10 weeks using either running, marching, and endurance-based circuit training (Circuits), or running, marching, and resistance training (Resistance). The march was performed before and after training, and other measurements related to loaded marching were conducted before training only. Each group was ranked by improvement in the loaded march, and divided into significantly different subgroups of 'good' and 'poor' responders (improvements of approximately 20% vs. 10%). For Circuits, there were significant differences between good and poor responders to training in the pretraining ratios of shuttle run: isometric lift strength (p = 0.031) and shuttle run: isometric back extension strength (p = 0.033). Stronger subjects with lower endurance responded better to Circuits. Resistance tended to show the opposite effect (p < 0.2). These are the first objective data on which to prescribe training for load carriage on an individual basis.

Adolescent↗

PubMed2003

Bradykinin receptor gene variant and human physical performance.

Accumulating evidence suggests that athletic performance is strongly influenced by genetic variation. One such locus of influence is the gene for angiotensin-I converting enzyme (ACE), which exhibits a common variant [ACE insertion (I)/deletion (D)]. ACE can drive formation of vasoconstrictor ANG II but preferentially degrades vasodilator bradykinin. The ACE I allele is associated with higher kinin activity. A common gene variant in the kinin beta(2) receptor (B(2)R) exists: the -9 as opposed to +9 allele is associated with higher receptor mRNA expression. We tested whether this variant was associated with the efficiency of muscular contraction [delta efficiency (DE)] in 115 healthy men and women, or with running distance among 81 Olympic standard track athletes. We further sought evidence of biological interaction with ACE I/D genotype. DE was highly significantly associated with B(2)R genotype (23.84 +/- 2.41 vs. 24.25 +/- 2.81 vs. 26.05 +/- 2.26% for those of +9/+9 vs. +9/-9 vs. -9/-9 genotype; n = 25, 61, and 29, respectively; P = 0.0008 for ANOVA adjusted for sex). There was evidence for interaction with ACE I/D genotype, with individuals who were ACE II, with B(2)R -9/-9 having the highest DE at baseline. The ACE I/B(2)R -9 "high kinin receptor activity" haplotype was significantly associated with endurance (predominantly aerobic) event among elite athletes (P = 0.003). These data suggest that common genetic variation in the B(2)R is associated with efficiency of skeletal muscle contraction and with distance event of elite track athletes and that at least part of the associations of ACE and fitness phenotypes is through elevation of kinin activity.

Adolescent↗

PubMed2002

Effects of resistance exercise volume and nutritional supplementation on anabolic and catabolic hormones.

Seven resistance-trained men performed six bouts of resistance exercise, each separated by at least 1 week, in a crossover design. High, moderate and low volumes of exercise were used, each performed twice and followed immediately post-exercise by either a placebo or carbohydrate-protein supplementation. All bouts of resistance exercise were performed using a load equal to 100% of each subject's ten-repetition maximum (10-RM), and all rest periods between sets of exercise were 1 min. Blood was obtained before and at intervals after exercise until 120 min post-exercise. Lactate levels were significantly (P < 0.05) elevated immediately post-exercise, and to a significantly greater extent after the greatest volume of exercise. Levels of growth hormone rose significantly after the greatest volume of exercise only. Those of insulin and glucose rose significantly after supplementation only. Cortisol levels tended to be higher after the greatest volume of exercise, but the differences were not significant. Supplementation had no effect on the lactate, growth hormone or cortisol responses to resistance exercise. The data indicate that volume of exercise and protein-carbohydrate supplementation can alter the metabolic and hormonal responses to resistance exercise independently. However, cortisol levels remain high after a high volume of resistance exercise, irrespective of whether a post-exercise carbohydrate-protein supplement is used.

Adult↗