[CD44v6 expression in er-negative and PgR-negative infiltrating ductal breast carcinomas].
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Adult↗
Biomedical subjects
Alvaro Ruibal
Publications and source records attributed to Alvaro Ruibal.
[Asymmetric pulmonary perfusion causing unilateral pulmonary edema as a complication of acute myocardial infarction].
Acute unilateral pulmonary edema is an unusual clinical condition occasionally associated with severe mitral valve insufficiency. We describe a patient diagnosed as having unilateral pulmonary edema after an acute anterior myocardial infarction. Echocardiograms performed in the acute phase ruled out mitral insufficiency. A perfusion lung scan showed left-sided pulmonary hypoperfusion. The diagnosis of acquired hypoplasia of the left pulmonary artery tree was made by chest computed tomography. The pathogenesis and differential diagnosis are discussed.
Aged↗
The variant E233G of the RAD51D gene could be a low-penetrance allele in high-risk breast cancer families without BRCA1/2 mutations.
Six SNPs have been detected in the DNA repair genes RAD51C and RAD51D, not previously characterized. The novel variant E233G in RAD51D is more highly represented in high-risk, site-specific, familial breast cancer cases that are not associated with the BRCA1/2 genes, with a frequency of 5.74% (n = 174) compared to a control population (n = 567) and another subset of breast cancer patients (n = 765) with a prevalence of around 2% only (comparison to controls, OR = 2.6, 95% CI 1.12-6.03; p < 0.021). We found that the immunohistochemical profile detected in available tumors from these patients differs slightly from those described in non-BRCA1/2 tumors. Finally, the structural prediction of the putative functional consequence of this change indicates that it can diminish protein stability and structure. This suggests a role for E233G as a low-penetrance susceptibility gene in the specific subgroup of high-risk familial breast cancer cases that are not related to BRCA1/2.
Age of Onset↗
[Axillary lymph node involvement in hormone-independent breast carcinomas].
BACKGROUND AND OBJECTIVE: We decided to study the axillary lymph node invasion (N) in hormone-independent breast cancer. PATIENTS AND METHOD: The study group included 77 ER and 47 ER/PgR breast carcinomas. Both ER and PgR were determined using EIAS from Abbott (USA). RESULTS: In ER tumors, one the most important finding was the high concentration (p = 0.001) and percentage of positives ( > 50 pmol/mg prot.; 24/41 vs 7/36; p < 0.01) of cathepsin D observed in N+ vs N- tumors. In ER/PgR negative tumors, lymph node involvement was associated with a same behavior of this proteinase (p = 0.021; 17/31 vs 2/16; p = 0.006) exclusively. CONCLUSIONS: Our results point to a possible usefulness of cathepsin D in this type of carcinomas.
Adult↗
[CD44s expression in squamous cell lung carcinomas].
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Adult↗
Dental diseases and radionuclide imaging of the jaws.
The aim of this study was to compare the results of radionuclide bone scans of the jaws with data obtained at the nuclear medicine department from a brief and feasible dental history, taking special account of cases with a positive scan and no recent dental events. Ninety-eight patients undergoing radionuclide bone scan as part of their diagnosis in non-dental, oncological and non-oncological diseases were imaged with 99mTc-labelled oxidronate. Superior and inferior halves of the mandible and maxilla (392 quadrants) were regarded as normal or having an abnormally high uptake. A recent (1 year) dental history was also obtained through a brief questionnaire and data were referred to each quadrant of the jaws. The association between the bone scan and dental disease was assessed by means of the chi-squared test. The overall results of scintigraphy and history coincided in 66 patients (46 with abnormal and 20 with normal findings; P = 0.002). Twenty-five patients had a positive scintigram without any known dental disorder. Results of scintigraphy and history coincided in 254 quadrants (78 with abnormal and 176 with normal findings; P < 0.001). Eighty-three quadrants had hot spots in the scintigram without any known dental lesion. It can be concluded that abnormal jaw scintigrams are frequent in patients without known dental disease, and this may indicate silent osteoblastic activity. These observations should be reported to the dentist for several reasons. First, they may reveal asymptomatic dental lesions. Second, the use of oral prostheses and implants is increasing and they require the support of healthy alveolar bone.
Adult↗
[Cytosolic cathepsin D levels in squamous carcinomas of the lung].
BACKGROUND AND OBJECTIVES: Cathepsin D is an aspartyl proteinase involved in tumoral invasion. The aim of this work was to study cathepsin D cytosolic levels in squamous carcinomas of the lung and their correlation with several clinical and biological parameters. PATIENTS AND METHOD: The study group included 95 squamous lung carcinomas and 38 normal tissue samples from the same patients. Cathepsin D cytosolic concentrations were determined using an immunoradiometric assay (CIS BioInternational. France). EGFR, erbB2 protein, CD44s, CD44v5 and CD44v6 levels at cell surfaces were determined. The clinical stage, histological grade, ploidy and S-phase cellular fraction (SP) were also considered as variables of the study. RESULTS: Cathepsin D cytosolic levels oscillated between 7.7 and 576 (median: 38.8) pmol/mg protein and were lower (p = 0.001) than those observed in 38 normal lung samples from the same patients. When tumors were classified according to different clinical and biological parameters, we noticed that cathepsin D levels were higher in carcinomas with lower proliferation rates and no nodal involvement, reaching statistical significance in both cases. Moreover, when lung carcinomas were classified according to cathepsin D concentrations, tumors with higher cathepsin D concentrations had lower EGFR levels (p = 0.011) and small global SP values (p = 0.025) and DNA index (p = 0.023). Likewise, they were found to be CD44s positive more frequently (p = 0.001) and SP positive less frequently (p = 0.022). CONCLUSIONS: These results lead us to suggest the following: a) in squamous carcinomas of the lung, cathepsin D cytosolic levels are lower than those observed in normal lung samples from the same patients, and b) in this subtype of lung carcinomas, high cathepsin D levels are associated with tumors without nodal involvement, with low proliferation rates, lower EGFR levels, and a reduced positivity for CD44s, pointing to a possible role of this proteinase as a parameter of good outcome.
Adult↗
[Clinical and biological differences between infiltrating ductal carcinomas of the breast in women over 70 years-old and those aged 60-70].
BACKGROUND: There is discrepancy in the literature regarding the behavior of breast cancer in elderly women. For this reason we decided to study the possible clinical and biological differences of infiltrative ductal breast carcinoma between women over 70 years and those aged between 60 and 70 years. PATIENTS AND METHOD: The study group included 181 women with infiltrative ductal carcinomas (IDCs) who were referred from a breast clinic (BC) and a breast cancer screening campaign (BCS). 67 of them were older than 70 and 114 were between 60 and 70 years old. Estrogen receptors (ER), progesterone receptors (PR), pS2 and cathepsin D cytosolic levels were determined, as well as levels of epidermal growth factor receptor (EGFR) in cell surfaces. Likewise, size, axillary lymph node involvement, distant metastasis, histological grade, ploidy and cellular S-phase fraction were also considered as variables of the study. RESULTS: IDCs in women older than 70 had greater size (p<0.001), global S-phase (p = 0.009) and number of axillary lymph nodes involved (p = 0.002). Likewise, these tumors showed more frequently distant metastasis (p = 0.001) and S-phase values >14% (p = 0.093). However, when we considered only women referred from BCS, no differences in the parameters studied between women older than 70 and those aged between 60 and 70 years were observed. When only patients coming from BC were considered, women older than 70 had a greater number of axillary lymph nodes involved (p= 0.027). Patients older than 70 years from BC showed greater size (p= 0.054) and more common distant metastasis (p= 0.075) than those from BCS. Also, patients between 60 and 70 referred from BC had tumors with greater size (p <0.001), nodal involvement (p= 0.022), number of lymph nodes involved (p= 0.022) and distant metastasis (p= 0.048) than those from BCS with the same age. CONCLUSIONS: Our results suggest that IDCs in women older than 70 years show clinical and biological features associated with a worse behaviour. However, this is only observed in women referred from BC but not in those coming from BCS. For this reason, the patient's referral site seems to be essential for the observed clinical and biological differences of breast carcinomas in the elderly.
Age Factors↗
[Usefulness of cytosolic tissue-type plasminogen activator levels in lung adenocarcinomas].
BACKGROUND: We aimed at studying the behavior of cytosol tissue-type plasminogen activator (t-PA) levels in lung adenocarcinomas and their correlation with other clinical and biological parameters. MATERIAL AND METHOD: t-PA cytosol levels were determined using EIA (Boehringer Mannheim. Germany) in 59 samples of lung adenocarcinoma and in 16 samples of normal lung tissue from the same patients. Cathepsin D, CA125, pS2, hyaluronic acid (HA), free beta subunit of chorionic gonadotropin hormone and neuron specific enolase (NSE) cytosol concentrations were determined. We also determined the concentrations of HA, erbB2 oncoprotein, epidermal growth factor receptor (EGFR), CD44s, CD44v5 and CD44v6 in cell surfaces. The following parameters were considered: clinical stage, ploidy, cellular S-phase fraction and histological grade. RESULTS: In adenocarcinomas, t-PA cytosol levels ranged from 0.1 to 14.6 ng/mg prot. (median, 1.4). These levels were lower than those in normal lung tissue (r, 0.1-18.6; median, 2.95 ng/mg prot.) but did not reach statistical significance. On the other hand, t-PA concentrations decreased as the clinical stage increased and were higher in stage I than stage II-III (p = 0.080) and stage III (p = 0.0622). No significant differences of t-PA levels were observed when the histological grade, ploidy and S-phase were considered. Adenocarcinomas with high t-PA values (> 3.7 ng/mg prot., representing the 75th percentile of the whole group), had lower CA125 (p = 0.015) and erbB2 oncoprotein (p = 0.087) concentrations. CONCLUSIONS: These results suggest that cytosol t-PA levels are negatively correlated with tumor size in lung adenocarcinomas. However, the usefulness of t-PA as a prognostic factor needs to be clarified in further studies.
Adenocarcinoma↗