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Amiram Grinvald

Publications and source records attributed to Amiram Grinvald.

22 records · Page 2Linked to original sources
PubMed2002

Dynamics and constancy in cortical spatiotemporal patterns of orientation processing.

How does the high selectivity to stimulus orientation emerge in the visual cortex? Thalamic feedforward-dominated models of orientation selectivity predict constant selectivity during the visual response, whereas intracortical recurrent models predict dynamic improvement in selectivity. We imaged the cat visual cortex with voltage-sensitive dyes to measure orientation-tuning dynamics of a large neuronal population. Tuning-curve width did not narrow after response onset, whereas the difference between preferred and orthogonal responses (modulation depth) first increased, then declined. We identified a suppression of the evoked responses, referred to as the evoked deceleration-acceleration (DA) notch, which was larger for the orthogonal response. Furthermore, peak selectivity of the tuning curves was contemporaneous with the evoked DA notch. These findings suggest that in the cat brain, sustained visual cortical processing does not narrow orientation tuning; rather, intracortical interactions may amplify modulation depth and suppress the orthogonal response relatively more than the preferred. Thus, feedforward models and recurrent models of orientation selectivity must be combined.

Animals↗
PubMed2002

Long-term voltage-sensitive dye imaging reveals cortical dynamics in behaving monkeys.

A novel method of chronic optical imaging based on new voltage-sensitive dyes (VSDs) was developed to facilitate the explorations of the spatial and temporal patterns underlying higher cognitive functions in the neocortex of behaving monkeys. Using this system, we were able to explore cortical dynamics, with high spatial and temporal resolution, over period of <or=1 yr from the same patch of cortex. The visual cortices of trained macaques were stained one to three times a week, and immediately after each staining session, the monkey started to perform the behavioral task, while the primary and secondary visual areas (V1 and V2) were imaged with a fast optical imaging system. Long-term repeated VSD imaging (VSDI) from the same cortical area did not disrupt the normal cortical architecture as confirmed repeatedly by optical imaging based on intrinsic signals. The spatial patterns of functional maps obtained by VSDI were essentially identical to those obtained from the same patch of cortex by imaging based on intrinsic signals. On comparing the relative amplitudes of the evoked signals and differential map obtained using these two different imaging methodologies, we found that VSDI emphasizes subthreshold activity more than imaging based on intrinsic signals, that emphasized more spiking activity. The latency of the VSD-evoked response in V1 ranged from 46 to 68 ms in the different monkeys. The amplitude of the V2 response was only 20-60% of that in V1. As expected from the anatomy, the retinotopic responses to local visual stimuli spread laterally across the cortical surface at a spreading velocity of 0.15-0.19 m/s over a larger area than that expected by the classical magnification factor, reaching its maximal anisotropic spatial extent within approximately 40 ms. We correlated the observed dynamics of cortical activation patterns with the monkey's saccadic eye movements and found that due to the slow offset of the cortical response relative to its onset, there was a short period of simultaneous activation of two distinct patches of cortex following a saccade to the visual stimulus. We also found that a saccade to a small stimulus was followed by direct transient activation of a cortical region in areas of V1 and V2, located retinotopically within the saccadic trajectory.

Animals↗
PubMedDate not supplied

Special report: Noninvasive multi-parameter functional optical imaging of the eye.

Advancement in the treatment of blindness depends on the development of new technologies that enable early detection, follow-up, and treatment of disease. The authors describe direct, noninvasive imaging of four parameters: blood flow, blood oximetry, metabolic state, and hidden vasculature, particularly capillaries. These are functional parameters of the retina known to be degraded by retinal disease. The new Retinal Function Imager (Optical Imaging, Ltd., Rehovot, Israel) can image all four parameters as intrinsic reflectance intensity differences over the retina's surface. During the past 2 decades, imaging of small optical signals has been a powerful tool for high-resolution functional mapping in the neocortex. In this article, this technology is applied to the retina and demonstrates a general tool for noninvasively probing retinal function in many modalities. Imaging functional changes before anatomic consequences arise holds promise as a powerful tool for early diagnosis and treatment of retinal disease.

Adult↗