Biomedical subjects

Ana I Cabañero

Publications and source records attributed to Ana I Cabañero.

5 recordsLinked to original sources

PubMed2006

Liquid chromatography coupled to isotope ratio mass spectrometry: a new perspective on honey adulteration detection.

A new procedure to determine individual sugar (sucrose, glucose, and fructose) 13C isotope ratios, using liquid chromatography-isotope ratio mass spectrometry (HPLC-IRMS), has been developed to improve isotopic methods devoted to the study of honey authenticity. For this purpose 79 commercial honey samples from various origins were analyzed. Values of delta13Choney ranged from -14.2 to -27.2", and delta13Cprotein ranged from -23.6 to -26.9". A very strong correlation is observed between the individual sugar 13C ratios, which are altered in the event of sugar addition, even at low levels. The use of Deltadelta13C [fruct-glu], Deltadelta13C [fruct-suc], and Deltadelta13C [gluc-suc] systematic differences as an authenticity criterion permits the sugar addition [C3, beet sugar; or C4, cane sugar, cane syrup, isoglucose syrup, and high-fructose corn syrup (HFCS)] to be reliably detected (DL = 1-10%). The new procedure has advantages over existing methods in terms of analysis time and sensitivity. In addition, it is the first isotopic method developed that allows beet sugar addition detection.

Carbon Isotopes↗

PubMed2006

Selenium long-term administration and its effect on mercury toxicity.

An in vivo experiment was conducted to assess selenium bioaccumulation and bioaccessibility through the food chain and its effect on Hg toxicity. For this purpose 72 chickens were fed under different controlled conditions. Chickens were exposed to a common basal diet or a diet supplemented with Hg(II), MeHg, and Se(IV). Enzymatic digestion (feed, chicken muscle, liver, and kidney) as well as simulated human gastric and intestinal digestion (chicken muscle) led to the identification of selenomethionine (SeMet) in all the samples analyzed. Therefore, although chickens have no efficient mechanism for SeMet synthesis they can be considered as a source of SeMet due to its diet and the plant-animal food chain. The kidneys were the target organ for both total Se and SeMet in chickens (1604 +/- 136 and 128 +/- 6 microg kg(-1), respectively), but the greatest body store, among the tissues studied, was the muscle in both cases (84-96% of total Se). Long-term administration of inorganic and organic mercury did not alter SeMet distribution significantly. The antagonistic effect of Se on Hg toxicity by favoring MeHg demethylation is discussed.

Animals↗

PubMed2005

Effect of animal feed enriched with Se and clays on Hg bioaccumulation in chickens: in vivo experimental study.

An in vivo experiment was conducted to evaluate the effects of sodium selenite, sepiolite, and bentonite on inorganic mercury (Hg) and methylmercury (MeHg) bioaccumulation. For this purpose 160 chickens were fed under different controlled conditions. Chickens were exposed to Hg(II) and MeHg added to feed with or without selenium or clays supplementation. No significant differences were observed in the voluntary intake and feed/gain conversion rates. The target organs of Hg(II) and MeHg in chickens were the liver and kidney, respectively, but the greatest body store was the muscle in both cases. A higher bioaccumulation for MeHg than for Hg(II) was observed. The results showed that addition of sodium selenite, sepiolite, or bentonite induced a decrease of up to 60-100% in the inorganic mercury bioabsorption. Bentonite addition to a MeHg-containing diet also caused a decrease in organic mercury bioaccumulation (29-67%). On the other hand, inorganic selenium and sepiolite did not decrease MeHg accumulation.

Aluminum Silicates↗

PubMed2005

Quantification and speciation of mercury and selenium in fish samples of high consumption in Spain and Portugal.

Mercury (Hg) and selenium (Se) determinations were carried out to evaluate human exposure to those elements through fish consumption in Spain and Portugal. Atomic fluorescence spectroscopy (AFS) was applied in a cold vapor mode for total mercury quantification and was also hyphenated to gas chromatography (GC) to achieve the speciation of organomercurial species in fish samples. The results obtained show the highest concentration of Hg in swordfish and tuna (0.47+/-0.02 and 0.31+/-0.01 microg g-1, respectively), and a much lower concentration in sardine, mackerel shad, and octopus (0.048+/-0.002, 0.033+/-0.001, and 0.024+/-0.001 microg g-1, respectively). The determination of alkyl mercury compounds revealed that 93-98% of mercury in the fish samples was in the organic form. Methylmercury (MeHg) was the only species found in the three fish species with higher mercury content. Total selenium concentration was high in sardine, swordfish, and tuna (0.43+/-0.02, 0.47+/-0.02, and 0.92+/-0.01 microg g-1, respectively), but low in mackerel shad and octopus (0.26+/-0.01 and 0.13+/-0.01 microg g-1, respectively). Speciation of selenium compounds was done by high-performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry (LC-ICP-MS). Selenomethionine (SeMet) was the only selenium compound identified in the fish samples with higher selenium content. Among the fish species studied, sardine had the most favourable Se:Hg and SeMet:MeHg molar ratios; therefore, its consumption seems to be preferable.

Animals↗

PubMed2004

Enzymatic probe sonication extraction of Se in animal-based food samples: a new perspective on sample preparation for total and Se speciation analysis.

This paper describes a fast, simple and novel extraction method for total selenium and selenium species determination in food samples. Parameters influencing extraction, such as sonication time, extracting media, temperature, sample mass, ultrasound amplitude and sample/enzyme mass ratio were investigated. The enzymatic hydrolysis proposed, enhanced by probe sonication, allowed the quantitative extraction of selenium in chicken muscle, liver, kidney and feed (97, 93, 95 and 102%, respectively) in 2 min, maintaining the original Se-species integrity. Total Se content of the samples was determined using inductively coupled plasma mass spectrometry. Se-species were identified and quantified using high-performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry. Chromatographic analyses were carried out under two chromatographic conditions and led to the identification of SeMet in all samples. The accuracy of the proposed method was assessed using certified reference materials as well as microwave digestion. Potential advantages of the proposed method over traditional hydrolysis are speed, simplicity and safety of the procedure.

Animal Feed↗