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Biomedical subjects

Andrew J Zele

Publications and source records attributed to Andrew J Zele.

10 recordsLinked to original sources
PubMed2006

Altered visual sensitivity in axial high myopia: a local postreceptoral phenomenon?

PURPOSE: The present study investigated retinal integrity in high myopia using spatial psychophysical tasks. METHODS: Ten axial high myopes (-8.5 to -11.5 D) and 10 age-matched control subjects (+/-1.0 D) were recruited. All participants underwent clinical examination and ocular biometry and demonstrated no visible macular disease with visual acuities better than 6/12. Foveal summation thresholds were determined for white and S-cone-isolating spots of various diameters up to 5.4 degrees and spatial contrast sensitivity to luminance sine wave gratings (0.5-9.7 cyc/deg). Data were analyzed after correction for the magnification induced by eye size and correcting lens power. RESULTS: Spatial summation for both white and S-cone-isolating spots showed a generalized loss of sensitivity at all spot sizes in myopes relative to control subjects (P = 0.01). Critical areas at maximum summation were significantly larger in myopes, for S-cone isolating spots only, after image size correction (P = 0.048). Sensitivity at maximum summation correlated negatively with vitreous chamber depth for both targets (P = 0.005). Sensitivities for S-cone and luminance spots also correlated (P < 0.001), indicating widespread dysfunction. Myopes displayed contrast sensitivity losses at high spatial frequencies (P </= 0.006) with a normal peak contrast sensitivity. CONCLUSIONS: These data can be interpreted to indicate that highly myopic eyes have either (1) a reduction in the number of receptors and/or a reduction in their sensitivity or, (2) a reduction in the sensitivity of postreceptoral processes. The presence of normal contrast sensitivity at low spatial frequencies indicates dysfunction at a postreceptoral level in high myopes.

Adolescent↗
PubMed2005

Disclosing disease mechanisms with a spatio-temporal summation paradigm.

BACKGROUND: We develop the logic for a stimulus that can evaluate cone-dependent spatial summation and detail the modelling and interpretation of thresholds obtained with this stimulus. METHODS: Fifteen observers participated, including two young normals tested extensively in control experiments, and a clinical trial based on four observers with age-related macular degeneration (AMD), four age-similar controls and five young observers. Monocular spatial summation functions were measured with contrast-modulated Gabor targets that approximated the optimal visual contrast detector. Thresholds were returned from a yes/no adaptive psychophysical algorithm. By fine titration along the size domain it was demonstrated that the spatial summation of normal observers can be adequately described by a two-component model. A reduced set of variables are proposed for clinical applications and the model was applied to data derived using these variables in persons with AMD and age-similar controls. RESULTS: We do not find a significant age-related loss of contrast sensitivity in our normal group. On the other hand, persons with early AMD exhibited a 0.41 log unit loss of sensitivity (P=0.04) from age-similar controls, without any change in their maximum summation area (A(max)). CONCLUSIONS: The nature of the spatial summation is consistent with the interpretation that early AMD produces a decrease in cone input to post-receptoral mechanisms in the absence of neural remodelling.

Adult↗
PubMed2005

Cathode-ray-tube monitor artefacts in neurophysiology.

We demonstrate that cathode-ray-tube (CRT) monitors commonly used as stimulus generators in visual neuroscience produce signal artefacts. This arises from two factors, one being the finite time needed for the raster scan of the CRT to cross the receptive field being stimulated, and the other being the restraint imposed by the impulse response of the phosphor itself. Together these factors result in smearing or blurring that manifests as high frequency noise, distorting the desired signal applied by the investigator. Our analysis identifies those conditions that promote these artefacts and we describe methods for their minimisation. We suggest that a monitor frame rate >/=100 Hz provides a reasonable trade-off between refresh and the generators of high frequency noise.

Artifacts↗
PubMed2004

Fos-tau-LacZ mice expose light-activated pathways in the visual system.

We have employed fos-tau-LacZ (FTL) transgenic mice to examine functional activation in the visual areas of the nervous system. The FTL mice express the marker gene lacZ in neurons and their processes following many different stimuli, and allow the imaging of activation from the level of the entire brain surface through individual neurons and their projections. Analysis of FTL expression in the retinas of mice following diurnal exposure to light shows that bipolar cells, specific classes of amacrine cells, ganglion cells, and a dense network of processes in the inner plexiform layer are functionally activated. In animals deprived of light, there is almost no activity in the retina. In the lateral geniculate nucleus (LGN), light exposure appears responsible for FTL expression in dorsal nuclei, but not for expression in the ventral nuclei or the intergeniculate leaflet. In the superficial layers of the superior colliculus, FTL expression is highly dependent on light exposure. Similarly, light exposure is required for FTL expression in primary visual cortex (area 17), but some expression remains in area 18 of dark-adapted animals. Finally, using mice with one or both eyes missing, we have determined which parts of the visual system are dependent on the presence of a functional connectivity from the eye. These data demonstrate the usefulness of the FTL mice to map functional activation within the entire visual system. Furthermore, we can capture visual activation in a conscious animal. Our findings give an insight into the architecture of activity within the retina and throughout the visual system.

Animals↗
PubMed2004

Chromatic and luminance losses with multiple sclerosis and optic neuritis measured using dynamic random luminance contrast noise.

We measured thresholds for detecting changes in colour and in luminance contrast in observers with multiple sclerosis (MS) and/or optic neuritis (ON) to determine whether reduced sensitivity occurs principally in red-green or blue-yellow second-stage chromatic channels or in an achromatic channel. Colour thresholds for the observers with MS/ON were higher in the red-green direction than in the blue-yellow direction, indicating greater levels of red-green loss than blue-yellow loss. Achromatic thresholds were raised less than either red-green or blue-yellow thresholds, showing less luminance-contrast loss than chromatic loss. With the MS/ON observers, blue-yellow and red-green thresholds were positively correlated but increasing impairment was associated with more rapid changes in red-green thresholds than blue-yellow thresholds. These findings indicate that demyelinating disease selectively reduces sensitivity to colour vision over luminance vision and red-green colours over blue-yellow colours.

Adult↗
PubMed2001

Fast psychophysical procedures for clinical testing.

INTRODUCTION: Psychophysical methods are used in clinical settings to obtain estimates of visual performance. Such methods should be fast and accurate, yet robust to the corrupting effects of false responses. METHODS: In this paper, we develop these concepts and investigate the efficiency of two maximum likelihood methods (bestPEST and ZEST) for use in clinical applications. The performance of both methods will depend on whether a criterion-free paradigm (alternate forced choice) is adopted. RESULTS: Our data show that the number of trials needed to obtain reliable thresholds with a yes/no paradigm can be as few as six to eight, provided no false responses are given within the first few trials. In addition, we show that the reliability and short-term variability of the endpoint of the methods is compatible with clinical applications. CONCLUSION: We show that a yes/no maximum likelihood method using a small number of presentations will yield reliable and accurate estimates of threshold in a clinical setting.

Journal Article↗
PubMedDate not supplied

Dark-adapted rod suppression of cone flicker detection: Evaluation of receptoral and postreceptoral interactions.

Dark-adapted rods in the area surrounding a luminance-modulated field can suppress flicker detection. However, the characteristics of the interaction between rods and each of the cone types are unclear. To address this issue, the effect that dark-adapted rods have on specific classes of receptoral and postreceptoral signals was determined by measuring the critical fusion frequencies (CFF) for receptoral L-, M-, and S-cone and postreceptoral luminance ([L+M+S] and [L+M+S+Rod]) and chromatic ([L/(L+M)]) signals in the presence of different levels of surrounding rod activity. Stimuli were generated with a two-channel photostimulator that has four primaries for a central field and four primaries for the surround, allowing independent control of rod and cone excitation. Measurements were made either with adaptation to the stimulus field after dark adaptation or during a brief period following light adaptation. The results show that dark-adapted rods maximally suppressed the CFF by approximately 6 Hz for L-cone, M-cone, and luminance modulation. Dark-adapted rods, however, did not significantly alter the S-cone CFF. The [L/(L+M)] postreceptoral CFF was slightly suppressed at higher surround illuminances, that is, higher than surround luminances resulting in suppression for L-cone, M-cone, or luminance modulation. We conclude that rod-cone interactions in flicker detection occurred strongly in the magnocellular pathway.

Color Perception↗
PubMedDate not supplied

Spatial and temporal chromatic contrast: Effects on chromatic discrimination for stimuli varying in L- and M-cone excitation.

Discrimination for equiluminant chromatic stimuli that vary in L- and M-cone excitation depends on the chromaticity difference between the test field and the surrounding area. The current study investigated the effect of the proximity in space and time of a surround to the test field on chromatic contrast discrimination. The experimental paradigm isolated spatial, temporal, and spatial-and-temporal chromatic contrast effects on discrimination. Chromatic contrast discrimination thresholds were assessed by a four-alternative spatial forced-choice procedure. Stimuli were either metameric to the equal energy spectrum, or varied in L-cone activation along a line of constant S-cone activation. A model based on primate parvocellular pathway physiology described the data. Spatial and temporal contrast produced equivalent reductions in chromatic discriminability as the chromatic difference between the test and surround increased. For all test chromaticities, discrimination was best in the absence of chromatic contrast. Chromatic contrast discrimination is determined by either the spatial or temporal contrast component of the signal.

Color Perception↗
PubMedDate not supplied

The color of night: Surface color perception under dim illuminations.

Several studies document rudimentary color vision under dim illumination. Here, hue perceptions of paper color samples were determined for a wide range of light levels, including very low light levels where rods alone mediate vision. The appearances of 24 paper color samples from the OSA Uniform Color Scales were gauged under successively dimmer illuminations from 10-0.0003 Lux. Triads of samples were chosen representing each of eight basic color categories; red, pink, orange, yellow, green, blue, purple, and gray. Samples within each triad varied in lightness. Observers sorted samples into groups that they could categorize with specific color names. Above 0.32 Lux, observers sorted the samples into the originally chosen color groups with few exceptions. For 0.1-0.01 Lux, the red and orange samples were usually correctly identified as either red or orange. The remaining samples tended to be grouped into two categories, associated with the scotopic sample reflectance. The lowest reflectance samples were below threshold and were named black. The higher reflectance group was named predominately as green or blue-green (three observers; the fourth observer used blue or achromatic). At the three dimmest levels (< or = 0.0032 Lux) there continued to be conspicuous color percepts. Color categories were reliably assigned based on relative sample scotopic lightness. Of the samples above threshold, those with lower reflectance were classified as red or orange (all observers) and the higher reflectance samples as green or blue-green (three observers) or achromatic or blue (the fourth observer). Rods and L-cones presumably mediated color percepts at the intermediate light levels used in the study. At the three lowest light levels there were distinct color appearances mediated exclusively by rods. We speculate that at these light levels the visual system estimates probable colors based on prior natural experience.

Color↗