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Andrew Wakefield

Publications and source records attributed to Andrew Wakefield.

3 recordsLinked to original sources
PubMed2005

Selectivity of cyclooxygenase isoform activity and prostanoid production in normal and diseased Han:SPRD-cy rat kidneys.

Renal prostanoids are important regulators of normal renal function and maintenance of renal homeostasis. In diseased kidneys, renal cylooxygenase (COX) expression and prostanoid formation are altered. With the use of the Han:Sprague-Dawley-cy rat, the aim of this study was to determine the relative contribution of renal COX isoforms (protein, gene expression, and activity) on renal prostanoid production [thromboxane B(2) (TXB(2), stable metabolite of TXA(2)), prostaglandin E(2) (PGE(2)), and 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha), stable metabolite of PGI(2))] in normal and diseased kidneys. In diseased kidneys, COX-1-immunoreactive protein and mRNA levels were higher and COX-2 levels were lower compared with normal kidneys. In contrast, COX activities were higher in diseased compared with normal kidneys for both COX-1 [0.05 +/- 0.02 vs. 0.45 +/- 0.11 ng prostanoids x min(-1) x mg protein(-1) (P < 0.001)] and COX-2 [0.64 +/- 0.10 vs. 2.32 +/- 0.22 ng prostanoids x min(-1).mg protein(-1) (P < 0.001)]. As the relative difference in activity was greater for COX-1, the ratio of COX-1/COX-2 was higher in diseased compared with normal kidneys, although the predominant activity was still due to the COX-2 isoform in both genotypes. Endogenous and steady-state in vitro levels of prostanoids were approximately 2-10 times higher in diseased compared with normal kidneys. The differences between normal and diseased kidney prostanoids were in the order of TXB(2) > 6-keto-PGF(1alpha) > PGE(2), as determined by higher renal prostanoid levels and COX activity ratios of TXB(2)/6-keto-PGF(1alpha), TXB(2)/PGE(2), and 6-keto-PGF(1alpha)/PGE(2). This specificity in both the COX isoform type and for the prostanoids produced has implications for normal and diseased kidneys in treatments involving selective inhibition of COX isoforms.

Animals↗
PubMed2003

A biomechanical comparison of facet screw fixation and pedicle screw fixation: effects of short-term and long-term repetitive cycling.

STUDY DESIGN: A biomechanical study was conducted to assess the stabilization performance of transfacet pedicle screw fixation. OBJECTIVE: To compare the biomechanical effects of short-term and long-term cyclic loading on lumbar motion segments instrumented with either a pedicle screw or a transfacet pedicle screw construct. SUMMARY OF BACKGROUND DATA: Facet screw fixation is an alternative to pedicle screw fixation that permits the use of a minimally invasive strategy. It is not known whether facet screw fixation can provide stability equivalent to pedicle screw fixation during cyclical loading. Therefore, transfacet pedicle screw fixation and standard pedicle screw fixation techniques were compared biomechanically. METHODS: Lumbar motion segments were tested under short-term and long-term cyclic loading conditions. For the short-term phase, specimens were tested intact for six cycles (to 400 N or 4 Nm) in compression, flexion, extension, lateral bending, and torsion. The specimens then were instrumented with bilateral semicircular interbody spacers and pedicle screw instrumentation or transfacet pedicle screws, and the testing sequence was repeated. For the long-term phase, 12 specimens were instrumented in a similar manner and loaded to 6 Nm of flexion bending for 180,000 cycles. RESULTS: For the short-term phase, both fixation systems had significantly greater stiffness and reduced range of motion, as compared with the intact state. No differences were observed between the fixation systems except in flexion, wherein transfacet pedicle screw specimens were significantly stiffer than traditional pedicle screw specimens. For the long-term phase, the stiffness and range of motion did not significantly increase or decrease over repetitive cycling of the instrumented specimens. Furthermore, no significant difference between the fixation systems was observed. CONCLUSIONS: The stability provided by both transfacet pedicle screw fixation and traditional pedicle screw fixation was not compromised after repetitive cycling. In this model, transfacet pedicle screw fixation appears equivalent biomechanically to traditional pedicle screw fixation.

Biocompatible Materials↗