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Biomedical subjects

Andrzej Kemona

Publications and source records attributed to Andrzej Kemona.

7 recordsLinked to original sources
PubMed2007

Connection between masticatory efficiency and pathomorphologic changes in gastric mucosa.

OBJECTIVE: The purpose of this investigation was to establish a connection between masticatory deficiency and pathomorphologic alterations of gastric mucosa in patients with dyspepsia. METHOD AND MATERIALS: In 40 dyspeptic, edentulous and partially dentate patients and in 40 dyspeptic control patients with good dental status, the Optosil masticatory function test was performed. Histopathologic changes of gastric mucosa and the severity of Helicobacter pylori infection were scored according to the updated Sydney Classification of Chronic Gastritis. RESULTS: The comminution of Optosil particles after increasing the number of chewing strokes was significantly impaired in edentulous and partially dentate patients, indicating masticatory deficiency in this group. This masticatory deficiency group was subdivided into groups with a lower (subgroup 1) and higher (subgroup 2) degree of masticatory deficiency according to median X50 in the Optosil test. The endoscopic appearance of gastric mucosa in masticatory deficiency patients and in the control group suggested chronic gastritis. Higher inflammatory and infection scores were noted in subgroup 2 than in the control group and subgroup 1, especially in the antral part of the stomach. The differences between the antral part and the body of the stomach were significant only in subgroup 2. CONCLUSIONS: Masticatory deficiency in patients with dyspepsia was connected with more severe chronic inflammatory changes and H. pylonri infection of gastric mucosa, especially in the antrum of the stomach. Higher values of inflammatory and infection scores in the subgroup with a higher degree of masticatory deficiency suggest its causal role in the promotion of gastritis and H. pylori infection.

Adult↗
PubMed2005

Transforming growth factor beta can be a parameter of aggressiveness of pT1 colorectal cancer.

AIM: To evaluate the significance of transforming growth factor beta (TGF beta) expression, in correlation with histopathological parameters, at the front of invasion in T1 colorectal cancer (CRC) and presence of metastases. METHODS: TGF beta immunohistochemical expression was studied in 34 specimens of colorectal adenocarcinomas (pT1). A three-step avidin-biotinylated immuno-peroxidase (ABCu-NCL) staining technique was performed on 4-mum paraffin-embedded tissue sections with a monoclonal antibody to TGF beta (Novocastra, NCL-TGFB, clone TGFB 17, dilution 1:40). RESULTS: Seventeen (50%) out of 34 lesions were positive for TGF beta expression. The TGF beta-positive rate in patients with vascular invasion was significantly higher than in those without vascular invasion (11/14 cases, P<0.01, P = 0.005). The TGF beta-positive rate was observed in 91.7% of patients with presence of tumor budding at the front of invasion (11/12 cases, P<0.01, P = 0.0003). A statistically significant correlation was found between the presence of lymph node metastases and positive expression of TGF beta (14/16 cases, P<0.01, P = 0.0001). We also observed that the TGF beta-positive rates in groups with distant and non-distant metastases were 92.8% and 20% respectively, and a significant correlation between TGF beta expression and distant metastasis was shown (P<0.01, P = 0.00003). CONCLUSION: The evaluation of TGF beta expression of protein in association with histological parameters can be used as a parameter of the aggressiveness of pT1 CRC.

Biomarkers, Tumor↗
PubMed2005

The effect of intragastric ammonia production on titratable gastric acid output in Helicobacter pylori-infected patients with chronic gastritis.

The purpose of this study was to assess whether intragastric neutralization of HCl by ammonia in Helicobacter pylori-infected patients could meaningfully affect the titratable acid output as a measure of gastric acid secretion in a relation to the severity of infection. In 79 patients with different degrees of Helicobacter pylori infection and chronic gastritis, the basal acid output (BAO) and maximal acid output (MAO) after pentagastrin (6 microg/kg s.c.) was estimated. Cl- and NH4+ contents in these fractions were also assayed. H+/Cl- ratio in the MAO fraction was diminished in markedly infected patients (68.1 +/- 3.9%, vs 84.1 +/- 3.3% in noninfected patients; P < 0.005). Ammonium content was maximal in patients with marked infection (0.912 +/- 0.086 vs 0.149 +/- 0.034 mmol/hr in MAO [P < 0.001] and 0.475 +/- 0.063 vs 0.105 +/- 0.016 mmol/hr in BAO of noninfected patients [P < 0.001]), with intermediate values in mild and moderate infection. The NH4 +/(H+ + NH4+) ratio reached 27.01 +/- 7.34% in the BAO of moderately infected patients, vs 10.22 +/- 3.81% in noninfected patients (P = 0.05), and 7.25 +/- 1.06% in the MAO of markedly infected patients, vs 1.14 +/- 0.33% in noninfected patients (P < 0.001). The intragastric ammonia production affects the titratable acid output in Helicobacter pylori-infected patients dependent on the severity of infection. Therefore this factor should be taken into consideration in the evaluation of gastric secretory function in Helicobacter pylori-infected patients.

Adult↗
PubMed2004

Effect of antibacterial therapy and salivary secretion on the efficacy of Helicobacter pylori eradication in duodenal ulcer patients.

OBJECTIVE: Oral health status may play a role in Helicobacter pylori eradication. Because adequate secretion of saliva promotes oral health, the aim of the study was to assess the effect of salivary secretion on the efficacy of H pylori eradication from the stomach. STUDY DESIGN: The study involved 90 H pylori-positive subjects with duodenal ulcer (68 men, 22 women, aged 20-70 years) in whom saliva was collected under basal conditions for 45 min before antibacterial treatment began. They received no drugs for at least 3 days prior to saliva collection. A 7-day course of either of 2 eradication regimens--omeprazole, amoxicillin, and tinidazole (OAT); or omeprazole, amoxicillin, and clarithromycin (OAC)--was used. The efficacy of eradication therapy was evaluated 30 days after its completion. RESULTS: The efficacy of H pylori eradication from the stomach (per protocol analysis) was 77.5% in the group of subjects treated with OAT and 81.6% with OAC. Combined analysis of both groups (OAT+OAC) showed reduced salivary secretion in subjects with eradication failure (0.395 +/- 0.266 vs 0.25 +/- 0.176 mL/min, P=.042). A similar outcome was obtained when the OAT group was analyzed separately (0.436 +/- 0.316 vs 0.211 +/- 0.216 mL/min, P=.022), but in the OAC group the difference was not significant. In the combined analysis, the efficacy of eradication therapy was lower in women than in men (52.9% vs 86.9%, P=.005). In women, it corresponded to salivary secretion (successful eradication 0.337 +/- 0.133 mL/min, unsuccessful eradication 0.180 +/- 0.144 mL/min, P=.043); whereas in men, the difference was not significant (successful eradication 0.405 +/- 0.282 mL/min, unsuccessful eradication 0.321 +/- 0.186 mL/min). CONCLUSION: Low salivary secretion may contribute to the decrease in efficacy of H pylori eradication from the stomach, at least in subjects treated with certain drug regimens.

Adult↗
PubMed2003

Mucosal adenosine deaminase activity and gastritis histology: a comparative study of partially resected and intact stomachs.

BACKGROUND: Adenosine deaminase is an enzyme which is postulated to have a role in the generation of gastric mucosal inflammation. The aim of our study was to determine and compare adenosine deaminase activity in the gastric mucosa of patients with chronic gastritis developed in partially resected and intact stomachs. MATERIAL/METHODS: 182 patients were studied, 102 non-operated and 80 after distal gastric resection. Biopsy specimens were taken endoscopically from the gastric mucosa 2 cm proximal to the stoma or corresponding upper third of the intact stomach. Gastritis was classified according to the Sydney system. The activity of adenosine deaminase in the mucosal homogenates was measured by determination of ammonia liberated from the substrate and expressed in nmol NH3/mg protein/min. RESULTS: Adenosine deaminase activity was lower in partially resected than in intact stomachs, regardless of Helicobacter pylori infection. While no difference was found in adenosine deaminase activity between Billroth I and Billroth II procedures in subjects without H. pylori infection, the activity was lower in those with Billroth II procedure in the presence of H. pylori infection. As the severity of gastritis increased, enzyme activity decreased in the mucosa of the intact stomach, but was not significantly altered in the mucosa of the gastric remnant. CONCLUSIONS: Adenosine deaminase activity differs in intact and partially resected stomachs, but it does not appear to be a factor promoting chronic gastritis.

Adenosine Deaminase↗
PubMedDate not supplied

[The role of cytokines in giardiasis in children].

INTRODUCTION: Trophozoites of Giardia lamblia present in the upper gastro-intestinal tract may cause inflammation of the duodenal mucosa, impair secretory activity of goblet cells, damage the enterocytes, and in case of intestinal villi atrophy, they may induce the malabsorption syndrome. The increase in number of endothelial lymphocytes in the epithelium of the duodenal mucosa indicates the involvement of local immune processes. THE AIM of the study was to assess the concentrations of chosen cytokines in the gastric mucosa in children infested with Giardia lamblia, before and after treatment, with simultaneous morphological evaluation of the duodenal mucosa before treatment. MATERIAL AND METHODS: The study included 12 children infested with Giardia lamblia and 15 children constituting controls. Giardiasis was diagnosed basing on the presence of trophozoites in the mucus taken from the duodenum during endoscopy (brush swab). In all patients, samples from the prepyloric section of the gastric and duodenal mucosa were examined histologically. In bioptates, concentrations of chosen cytokines were determined by ELISA method. RESULTS: Histopathological examination of the gastric mucosa showed chronic antral gastritis in 8.3% and duodenal mucosa inflammation in 58.3%. Mean concentrations of cytokines determined in the antral mucosa before treatment equaled: IL-2 - 53.1; IL-4 -27.7; IL-5 -39.4; IL-8 - 41.4; IL-10 - 33.0; IFN-gamma - 60.3; TNF-alpha - 27.7 pq/mg of protein. Concentrations of mucosal cytokines, apart from IL-10 (82.7 pq/mg of protein), decreased due to targeted treatment with the simultaneous improvement of histopathological changes reported before treatment. CONCLUSION: In children infested with Giardia lamblia, an increased local production of proinflammatory and proallergic cytokines was observed in the gastric mucosa.

Animals↗