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Andrzej Pucher

Publications and source records attributed to Andrzej Pucher.

12 recordsLinked to original sources
PubMed2006

Expression of osteoblast marker genes in rat calvarial osteoblast-like cells, and effects of the endocrine disrupters diphenylolpropane, benzophenone-3, resveratrol and silymarin.

Compelling evidence indicates that some endocrine disrupters (EDs), acting as selective estrogen-receptor modulators, interfere with osteoblast differentiation and function. Hence, we investigated whether four EDs [bisphenol-A (BSP), benzophenone-3 (BP3), resveratrol and silymarin] affect differentiation and growth of rat calvarial osteoblast-like (ROB) cells in primary in vitro culture. ROB cells were cultured for up 30 days in a medium supplemented with fetal calf serum (FCS), and conventional RT-PCR detected the expression of collagen-1alpha and osteonectin mRNAs through the entire culture period. Real time-PCR demonstrated that at days 2 and 7 of culture the expressions of collagen-1alpha and osteonectin were very low, and underwent a 192- and a 334-fold increase, respectively, at day 21 of culture. In contrast, osteocalcin expression remained unchanged from days 2 to 21 of culture. EIA showed that ROB cells secreted sizeable amounts of osteocalcin and osteopontin between days 13 and 15 of culture. EDs were added at day 13 of culture at concentrations ranging from 10(-10) to 10(-6) M, being the culture medium deprived of FCS, and their effects were tested 48 h later. None of EDs was found to affect osteocalcin and osteopontin secretion from ROB cells, suggesting that their effects were tested at a relatively earlier stage of culture, when ROB cell differentiation into osteoblats is not fully accomplished, and/or the presence of estrogens contained in FCS is needed for EDs to exert their osteoblast-differentiation modulating action. BSP and BP3, but not resveratrol and silymarin, decreased proliferative activity of cultured ROB cells, a cytotoxic effect conceivably independent of their estrogen-receptor modulating activity.

Animals↗
PubMed2006

Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1alpha gene expression in primary culture of rat calvarial osteoblast-like cells.

Evidence is available that some endocrine disruptors, acting as selective estrogen receptor modulators (SERMs), interfere with osteoblast differentiation and function. Therefore, we investigated whether 17beta-estradiol, bisphenol-A (BSP), silymarin, genistein, resveratrol, procymidone, linurone and benzophenone-3 (BP3) modulate differentiation of rat calvarial osteoblast-like (ROB) cells in primary in vitro culture. Disruptors were added at day 18 of culture and cells were harvested 48 h later. Real time-PCR revealed that estradiol and resveratrol enhanced osteocalcin mRNA expression in ROB cells, while other disruptors were ineffective. The expression of osteonectin and collagen-1alpha was not affected by any disruptor. Estradiol, resveratrol, genistein and BSP stimulated the proliferative activity of ROB cells. In contrast, procymidone and linurone inhibited the proliferative activity, and silymarin and BP3 were ineffective. The conclusion is drawn that i) only resveratrol is able, like estradiol, to stimulate the specialized functions of ROB cells, and ii) the proliferative activity of ROB cells is more sensitive to endocrine disruptors, some of which could probably act via a mechanism independent of their SERM activity.

Animals↗
PubMed2005

[Long-term results of pinning for slipped capital femoral epiphysis].

Clinical and radiological analysis of 39 hips in 32 patients treated with in situ pinning for light and non-severe (up to 78 degrees) slipped capital femoral epiphysis. The population of 21 boys and 11 girls aged 10-16 (av. 13.1) was observed in 4-27 (av. 21) years. The observation showed 18.8% very good, 18.8% good, 46.8% satisfactory, 12.5% bad results and 3.1% (1 patient) of unsuccessful treatment, according to Heyman & Herndon clinical evaluation scale. Radiological evaluation of secondary coxarthritis acc. to Boyer: 0 - 35.,8%, I - 23.1%, II - 33.4%, III - 7.7%. Slipped upper femoral epiphysis causes secondary coxarthritis. Kirschner wire pinning is good method for non-severe cases.

Adolescent↗
PubMed2005

[Axial and derotational traction before surgery for slipped femoral capital epiphysis].

18 cases of slipped femoral capital epiphysis were prepared for surgery by axial and derotational traction lasting 1 to 5 (av. 2,9) weeks. In all cases significant pain relief was achieved, partially the range of passive motion in the hip was improved. No reduction of slipped epiphysis was recorded. Best results occurred in medium and severe (> 60 degrees) cases. The traction before surgery for slipped femoral epiphysis is considered to improve range of motion, bring good pain relief, but not reduce the slipped epiphysis.

Adolescent↗
PubMed2005

[Aseptic necrosis of proximal femur in children undergone early non-operative treatment of DDH].

Aseptic necrosis of proximal femur is the most serious complication of DDH conservative treatment. 72 dysplastic hips in 48 girls and 8 boys have been evaluated. the hips were Grafs IIc, D, III and IV types, and the treatment started between 2nd and 12th week of the newborns age. The hips have been observed until the children started walking (average 13 months). The early treatment did not eliminate femoral osteochondrosis, but the diagnosed cases were mainly not severe according to Kruczyński.

Female↗
PubMed2005

[The effect of absent ossific nucleus of the femoral head on results of surgical treatment for developmental dislocation of the hip].

We have studied retrospectively 33 hips in 26 children at an average of 9 years 5 months after simultaneous open reduction and Dega transiliac osteotomy for developmental dislocation of the hip (DDH). All children were younger than 2 years of life at the time of surgery. The hips were divided into two groups: with present and with absent ossific nucleus of the femoral head on preoperative radiographs. Clinical and radiographic results have been assessed in both groups. No statistically significant difference was found between the groups. We conclude, that absent ossific nucleus of the femoral head as a sole reason should not delay surgery for DDH.

Child↗
PubMed2004

[Sonographic assessment of stable hip reposition in newborns and infants with congenital hip dysplasia treated conservatively].

The paper presents a sonographic assessment of dysplastic hip morphology with clinically diagnosed instability after achieving stable reposition. The analysed group comprised 42 dysplastic unstable hips. They were classified according to the Graf classification as type D, III and IV. The analysed group was divided into 2 subgroups according to the child's age at the time treatment was began. Group I comprised children in whom treatment was began at age 2-6 weeks. Group II included children who were 7-12 weeks old at the time treatment was began. The morphology of the dysplastic hip was assessed basing on angle alpha and beta measurements. Stable repositioning of the hip was achieved on average 5 weeks after conservative treatment was started. The average alpha angle was 50 degrees in group I and 48 degrees in group II; accordingly, angle beta was on average 60 degrees and 69 degrees.

Acetabulum↗
PubMed2002

[Surgical treatment of advanced congenital scoliosis with the C-D method].

The paper presents the results of surgical treatment of 15 cases of congenital scolisios with CD instrumentation. Progression of the deformity was most often seen in cases with combined deformities (according to the McMaster classification). Indications for surgical treatment were progression of the curvature and trunk and thorax deformity progression, particularly during the growth spurt. The age at the time of surgery ranged from 10.3 to 16 years (average: 13.8 years). Follow-up period ranged from 2 to 8 years (average: 4 years). Intraoperative deformity correction ranged from 0 to 60% (average 32%). Loss of correction during follow up ranged from 0 to 3%. The overall silhouette improvement was a result of correction of the spine curvature, correction of thoracic hyperkiphosis and a decrease of trunk decompensation. The only complications noted were 3 cases of transient neurological symptoms from the lower extremities. Preoperative MRI allows detection of congenital deformities of the spinal canal, a contraindication for surgical correction of the deformity with spinal implants.

Cervical Vertebrae↗
PubMedDate not supplied

Simultaneous open reduction and Dega transiliac osteotomy for developmental dislocation of the hip in children under 24 months of age.

The authors studied retrospectively 33 hips in 26 children at an average of 9 years 5 months after simultaneous open reduction and Dega transiliac osteotomy for developmental dislocation of the hip (DDH). All children were younger than 2 years of life at surgery. At the latest follow-up 89% of the clinical results and 72% of the radiographic results were rated excellent or good, despite a 45% rate of avascular necrosis observed before surgery. The authors attempted to identify factors influencing the final result. Given the low complication rate (3%) and low reoperation rate (3%), the authors conclude that the reviewed surgical protocol is safe and efficient in the treatment of DDH in young children.

Bone Diseases, Developmental↗
PubMedDate not supplied

[Surgical treatment of degenerative lumbar spondylolisthesis].

BACKGROUND AND PURPOSE: Degenerative lumbar spondylolisthesis occurs most often at the L4-L5 level and is characterized by anterior displacement of L4 vertebra on L5 vertebra. The aim of the study is to present both the decompression of nerve elements of the vertebral canal and the method of spine internal stabilization in patients with degenerative lumbar spondylolisthesis. MATERIAL AND METHODS: Clinical analysis comprises 11 patients treated surgically because of the degenerative spondylolisthesis. Intervertebral dislocation of more than 20% included 4 patients where the vertebral column stabilization was achieved by transpedicular stabilization as well as intervertebral and posterolateral spinal fusion (group I). In spondylolisthesis of less than 20% (4 patients) spondylodesis with intervertebral cages and posterolateral osseous grafts were carried out (group II). In the remaining 3 cases spondylolisthesis was smaller than 20% with denivelation of the intervertebral space (group III). There were nervous structures deliberated and spondylodesis was unnecessary. RESULTS: Low back pain and sciatic neuralgia were relieved in 9 (82%) patients. Neurological deficits were subsided completely in 7 (78%) cases. CONCLUSIONS: The evaluation of the degree of vertebral displacement, height of the intervertebral space and assessment of vertebral column stability allowed to determine the strategy of surgery in patients with degenerative lumbar spondylolisthesis.

Decompression, Surgical↗