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Andy Kerr

Publications and source records attributed to Andy Kerr.

6 recordsLinked to original sources
PubMed2007

Large-scale mapping of mutations affecting zebrafish development.

BACKGROUND: Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers. RESULTS: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM. CONCLUSION: By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.

Animals↗
PubMed2006

Timing phases of the sit-to-walk movement: validity of a clinical test.

The sit-to-walk (STW) movement is a functional task that challenges balance and co-ordination. There is a paucity of literature investigating the phases of this movement and its significance in a clinical rehabilitation context. Measuring phases of this movement may provide clinically applicable data for screening subjects for mobility problems and evaluating interventions. Fifty-six subjects from three groups; young (<65 years old), elderly (>65 years old) and elderly at risk of falling (EARF), performed the STW movement freely from a chair. Switches placed on the backrest, chair seat and two on the floor identified the times of movement events: onset, seat-off, swing-off and stance-off. These events defined three phases: flexion, extension and stance. Timing of events and phase duration data derived from this switch system were correlated with those taken from a three-dimensional motion analysis system. All switch events closely matched the motion analysis events with ICC (model 2.1) scores ranging from 0.93 to 1.00. Duration of all STW phases were statistically longer in the EARF group compared to both unimpaired groups (p<0.05). Data from the four switch configuration demonstrated excellent concurrent validity when associated with data from a three-dimensional motion analysis system in identifying the phases of STW. Measurement of the phases of the STW task has potential in screening those at risk of falling and informing care strategies to prevent falls.

Adult↗
PubMed2004

Defining phases for the sit-to-walk movement.

OBJECTIVE: To define phases of the sit-to-walk movement and test their consistency in a normal population. DESIGN: An observational study of thirteen healthy volunteers. BACKGROUND: Moving from sitting to walking is a daily activity that may present difficulty for some populations in terms of control and stability. METHODS: The movement was partitioned into phases according to changes in ground reaction forces and peak velocity of the total body centre of mass. Consistency of each phase duration was assessed. RESULTS: Four phases of sit-to-walk were defined; flexion momentum, extension, unloading and stance. ICC scores for phase duration ranged from 0.54 (extension) to 0.81 (stance). CONCLUSIONS: This is the first study to define distinct phases of the sit-to-walk movement. There was moderate to good consistency for phase duration. RELEVANCE: By defining and testing phases of the sit-to-walk movement this study enhances understanding of this everyday movement and provide a model for future research.

Activities of Daily Living↗
PubMedDate not supplied

Has packing sinus wounds become a ritualistic practice?

Wound healing is both a science and an art, and modern advances in wound management are ensuring that advanced clinicians use both clinical knowledge and experience when deciding on wound dressings and therapies. When there is a lack of evidence, then selection of dressings or therapies becomes extremely difficult and inappropriate treatment can occur. This is generally the situation with sinus wounds. There is very little evidence available to help decide on suitable dressings for these intractable wounds. The use of high frequency ultrasound (HFU) can help to guide practice on whether or not to pack sinus wounds. This article will explore the potential of ultrasound use by describing a case study of a patient with a sinus wound where HFU was used to examine the sinus and inform the practitioners on the appropriate choice of wound dressing.

Aged↗
PubMedDate not supplied

'Gonnae no dae that'.

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Health Education↗