Anil Kapoor — Page 2 | PubMed Health

PubMed2003

Technique of laparoscopic nephrectomy in a patient with previous urinary diversion.

Laparoscopic nephrectomy is quickly becoming accepted as the standard treatment for small renal neoplasms and benign renal disease. The presence of an ileal conduit has been termed a relative contraindication to laparoscopic surgery. A 58-year old female presented with recurrent pyelonephritis and hydronephrosis of her left kidney. Surgical removal was necessary and we considered a transperitoneal laparoscopic nephrectomy for her. The patient had an ileal conduit and it was because of this reason that only after careful consideration was it decided to attempt the surgery using the laparoscopic approach. We present our technique of transperitoneal laparoscopic nephrectomy in this patient with an ileal conduit and include technical suggestions that will help predict a successful outcome. The success of this case demonstrates that in certain circumstances, patients with urinary diversions can be offered laparoscopic nephrectomy and its benefits.

Female↗

PubMed2002

Apoptotic release of histones from nucleosomes.

Chromatin structure is influenced by histone modification, and this may help direct chromatin behavior to facilitate transcription, DNA replication, and DNA repair. Chromatin condensation and DNA fragmentation are the classic nuclear features but remain poorly characterized. It is highly probable that nucleosomal structure must be altered to allow these features to become apparent, but data to support this construct are lacking. We report here that in response to apoptotic signals from a death receptor (CD95 and tumor necrosis factor-alpha) or mitochondrial (staurosporine) apoptotic stimulus, the core nucleosomal histones H2A, H2B, H3, and H4 become separated from DNA during apoptosis in Jurkat and HeLa cells and are consequently detectable in the cell lysate prepared using a non-ionic detergent. The timing of this histone release from DNA correlates well with the progression of apoptosis. We also show expression of a caspase cleavage-resistant form of ICAD (ICAD-DM) in Jurkat and HeLa cells abolished DNA fragmentation and also dramatically reduced histone release in apoptotic cells. However, we demonstrate that apoptotic histone release is not an inevitable consequence of CAD/DFF-40-mediated DNA destruction as DNA fragmentation but not histone release occurs efficiently in tumor necrosis factor-alpha- and etoposide-treated NIH3T3 cells. Furthermore, in an in vitro apoptotic assay, incubation of apoptotic Jurkat cellular extract with non-apoptotic Jurkat nuclei led to nuclear DNA fragmentation without obvious histone release. Taken together, these data demonstrate that CAD/DFF-40 functions indirectly in mediating nucleosomal destruction during apoptosis.

3T3 Cells↗