Biomedical subjects

Anirban Sen Gupta

Publications and source records attributed to Anirban Sen Gupta.

3 recordsLinked to original sources

PubMed2005

RGD-modified liposomes targeted to activated platelets as a potential vascular drug delivery system.

Local drug delivery has become an important treatment modality for the prevention of thrombotic events following coronary angioplasty. In this study, we investigate the ability of liposomes bearing surface conjugated linear Arg-Gly-Asp (RGD) peptide (GSSSGRGDSPA) moieties to target and bind activated platelets, and the effect of such RGD-modified liposomes on platelet activation and aggregation. The binding of RGD-liposomes to human platelets was assessed by fluorescence microscopy, phase contrast microscopy and flow cytometry. The effect of RGD-modified liposomes on platelet activation and aggregation was investigated in vitro, with and without platelet agonists. RGD-liposomes were found to bind activated platelets at levels significantly greater than the control RGE-liposomes. The RGD-liposomes did not exhibit any statistically significant effect on platelet activation or aggregation. The results demonstrate the ability of the RGD-modified liposomes to target and bind activated platelets without causing significant platelet aggregation and suggests a feasible way for the development of a platelet-targeted anti-thrombogenic drug delivery system. Furthermore, the approach can be extended to the development of liposomes for other vascular targets, for application in drug delivery or gene therapy.

Blood Platelets↗

PubMed2003

Polyphosphates and other phosphorus-containing polymers for drug delivery applications.

Poly(phosphate ester)s, polyphosphonates, and polyphosphazenes are three classes of phosphorus-containing polymers that have received wide attention over the past decade for their utility in biomedicine and tissue engineering. These three families of polymers can lead to a number of subclasses of polymers with varied properties. Significant research in this area has led to niche polymers with morphologies ranging from viscous gels to amorphous microparticles for utility in drug delivery. Furthermore, the pentavalency of phosphorus offers the potential for covalent linking of the drug. The classes of polymers discussed in this review are being explored in human clinical trials for vaccine delivery as well as delivery of oncolytic and CNS therapeutics. More applications in the areas of DNA delivery and tissue engineering are also being explored.

Delayed-Action Preparations↗

PubMed2002

L-tyrosine-based backbone-modified poly(amino acids).

Tyrosine-based pseudo-peptide polymers, first introduced in 1987 by Kohn and Langer, have been identified for potential biomaterial applications. These materials combine the desired polypeptide properties of biocompatibility, biodegradability, non-toxicity, and non-immunogenicity with good processing properties including solubility, thermal stability, and moldability which arise from alternating non-amide bonds along the polymer backbone. This paper focuses on the analysis of two such polymers based on the natural amino acid L-tyrosine. Starting from L-tyrosine and its deaminated analogue, 3-(4-para-hydroxy)-phenylpropionic acid, a diphenolic structure containing an amide linkage, was synthesized following standard procedures of peptide synthesis. This diphenolic structure was then used as a monomer to synthesize a polyiminocarbonate using a cyanogen bromide-initiated reaction and a polycarbonate using a triphosgene-initiated reaction. The polyiminocarbonate has iminocarbonate linkages and the polycarbonate has carbonate linkages alternating with amide linkages in the respective polymer backbone. Analytical studies were performed regarding the feasibility of the reaction procedures, the physical properties of the polymers, and their degradation processes, to gain insight into the potential biomaterial applications of these polymers. These results independently reaffirm the studies published by Kohn et al. working on similar polymeric systems.

Biocompatible Materials↗