Biomedical subjects

Ann Wright

Publications and source records attributed to Ann Wright.

4 recordsLinked to original sources

PubMed2005

Comparative cytotoxicity potential of soft contact lens care products.

PURPOSE: To determine the cytotoxicity potential of soft contact lens care products and benzalkonium chloride (BAK) by two colorimetric in vitro assays on an immortalized human corneal epithelial cell line (HCE-T). METHODS: Four commercial soft contact lens care solutions were tested at 1:3 dilution in growth medium. The positive controls for cytotoxicity were BAK in Dulbecco's phosphate buffered saline (DPBS) at 10, 5, 2.5, and 1.25 ppm and 1:3 dilution of DPBS as negative control. Cell viability was assayed using a novel tetrazolium compound and an electron coupling reagent (MTS/PES), and by cell membrane integrity using neutral red dye uptake and release (NRUR). Mean spectrophotometric optical density of the test samples was compared to mean optical density of the controls at 24 and 48 hours of exposure to test product. Significance was measured by ANOVA/Tukey HSD test. RESULTS: Cell viability and cell membrane integrity tests were not significantly different between lens care solutions and the negative controls for CIBA Vision SOLOcare PLUS, Advanced Medical Optics COMPLETE Comfort PLUS, or BAK at 1.25 ppm. Exposure to Bausch and Lomb ReNu MultiPlus, Alcon OPTI-FREE Express with Aldox, BAK 10, 5, and 2.5 ppm yielded significant reduction in cell viability and membrane integrity compared to negative controls. DISCUSSION: Assays of HCE-T cell viability by MTS/PES, and cell membrane integrity by NRUR cells yielded data that were similar to that previously reported with mouse L929 cells in tests based on the USP Elution Test. For the MTS/PES and NRUR assay methods, the solutions in order of increasing cytotoxicity potential were: SOLOCare = COMPLETE Comfort Plus < ReNu < < OPTI-FREE Express with Aldox.

Journal Article↗

PubMed2002

Plasma tissue inhibitor of metalloproteinase-1 levels are elevated in essential hypertension and related to left ventricular hypertrophy.

BACKGROUND: Essential hypertensive patients have an increased heart and arterial collagen concentration. Increased collagen synthesis can be assessed using procollagen III N peptide (PIIINP) and reduced collagen degradation measured using tissue inhibitor of metalloproteinase-1 (TIMP-1). METHODS: Plasma TIMP-1 and PIIINP levels were measured in 31 patients with essential hypertension and in 17 normotensive control subjects. The hypertensive patients were either treatment naive (n = 18) or had been without treatment for 1 month (n = 13). Both groups of patients were screened to exclude other fibrotic diseases. RESULTS: In the hypertensive patients, TIMP-1 levels were significantly (P < .0002) elevated (median 380 ng/ mL, range 160 to 1,560 ng/mL) compared with those of the normotensive control subjects (median 178 ng/mL, range 99 to 330 ng/mL). In hypertensive subjects who had never received antihypertensive therapy there were significant correlations between TIMP-1 and left ventricular posterior wall thickness in diastole (LVPWd) (r = 0.58) (P < .02) and left ventricular mass index (r = 0.58) (P < .02). There was no difference in PIIINP levels (mean +/- 2 SD) between the hypertensive (0.56 U/mL +/- 0.3) and normotensive groups (0.52 U/mL +/- 0.2). CONCLUSIONS: The increased tissue collagen III levels found in the heart and vessels of hypertensive patients is due to a reduction in collagen degradation because of high TIMP-1 levels, rather than an increase in synthesis of collagen type III. The tissue source of this TIMP-1 is unclear.

Female↗

PubMed2002

Comparative cytotoxicity potential of soft contact lens care regimens.

PURPOSE: To determine the cytotoxicity potential of soft contact lens disinfection solutions. METHODS: Three modifications of the United States Pharmacopeia (USP) elution test were conducted: trypan blue uptake test; regrowth of cells after exposure; and quantitation of viable cells after exposure test. Cycled lenses were also tested according to the USP direct-contact test. We compared the cytotoxicity profile of neutralized AOSept (CIBA Vision, Duluth, GA) disinfectant, SOLO-care Soft (CIBA Vision, Duluth, GA) brand multipurpose solution, OPTI-FREE Express (Alcon, Ft. Worth, TX) multipurpose disinfecting solution (with ALDOX), ReNu (Bausch & Lomb, Rochester, NY) multipurpose solution, ReNu MultiPlus (Bausch & Lomb, Rochester, NY) multipurpose solution, and COMPLETE Comfort PLUS (Allergan, Irvine, CA) multipurpose solution. Appropriate positive and negative controls were used for each test. RESULTS: Neutralized AOSept, SOLO-care soft, and COMPLETE Comfort PLUS solutions were noncytotoxic by all four test methods. ReNu MPS and ReNu MultiPlus both were noncytotoxic by the USP direct contact test and the USP elution-based trypan blue uptake and cell regrowth tests, but both yielded less than 50% of viable cells. In the three USP Elution test methods, OPTI-FREE Express (with ALDOX) exhibited cytotoxicity. CONCLUSIONS: These solutions have shown widely varying cytotoxicity potential. Neutralized AOSept, SOLO-Care Soft, and COMPLETE Comfort Plus were noncytotoxic by all four tests. ReNu MultiPlus and ReNu MPS inhibited the growth of cells after exposure. OPTI-FREE Express (with ALDOX) may have a higher potential for ocular irritation correlating to severe cytotoxicity in vitro.

Animals↗

PubMedDate not supplied

Precepting in 2002.

Preceptorships have been used to bridge the gap between nursing education and the reality of the workplace. The role of preceptors to prepare new or transitioning nurses to function more efficiently and earlier has become increasingly important in recent years. To foster positive attitudes in the experienced nurses toward accepting and functioning in the roles of preceptors, nursing administration and nursing education must collaborate. Preceptors have the ability to shape, nurture, influence, and support novice nurses. Experienced nurses hold the responsibility to encourage this commitment.

Attitude↗